Intestinal Morphology and Microbiology of Broiler Chicken Fed Diets Containing Myrtle (Myrtus communis) Essential Oil Supplementation

Iranian Journal of Applied Animal Science

مقاله 14، دوره 4، شماره 3، آذر 2014، صفحه 549-554   

نوع مقاله: Research/Original Article

نویسندگان

S. Ghazanfari^{* 1}؛ M. Adib Moradi²؛ M. Mahmoodi Bardzardi¹

¹Department of Animal Science, College of Abouraihan, University of Tehran, Tehran, Iran

²Department of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

چکیده

This experiment was conducted to determine the effects of dietary myrtle essential oil on small intestinal morphology and microbiology of broiler chickens. Two hundred day-old broiler chickens (Ross 308) were allocated to 5 treatments, 4 replications with a completely randomized design. The diets were supplemented with: no myrtle essential oil (control), each of myrtle essential oil at levels of 100, 200 and 300 mg/kg and 600 mg/kg flavophospholipol antibiotic of diet. On day 42 days of age, one bird from each replicate was selected and then slaughtered and the intestinal microflora contents as well as morphology were analyzed. Dietary myrtle essential oil and antibiotic supplementations consistently resulted in significantly longer villus height, lower epithelial thickness and lower goblet cell number of the small intestinal at 42 day of age compared with the control treatment (P<0.05).Also, lower Escherichia coli count and higher Lactobacillus count of the caecum was found by inclusion of myrtle essential oil in the diet (P<0.001). The results of this study suggested that myrtle essential oil may be considered as an alternative to antibiotic growth promoter may improve poultry performance.

کلیدواژه‌ها

Broiler؛ gut morphology؛ Microbiology؛ myrtle essential oil

مراجع

Akin M., Aktumsek A. and Nostro A. (2010). Antibacterial activity and composition of the essential oils of eucalyptus camaldulensis dehn and Myrtus communis growing in nothern Cyprus. African J. Biotechnol. 9, 531-535.
Aviagen. (2002). Ross Broiler Management Manual. Aviagen Ltd., Newbridge, Scotland.
Bedford M. (2000). Removal of antibiotic growth promoters from poultry diets: implications and strategies to minimize subsequent problems. World Poult. Sci. J. 56, 347-365.
Biricik H., Yesilbag D., Gezen S.S. and Bulbul T. (2012). Effects of dietary myrtle (Myrtus communis) supplementation on growth performance, meat oxidative stability, meat quality and erythrocyte parameters in quails. Rev. Med. Vet. 163, 131-138.
Brenes A. and Roura E. (2010) Essential oils in poultry nutrition: main effects and modes of action. Anim. Feed. Sci. Technol. 158, 1-14.
Chryssavgi G., Vassiliki P., Athanasios M., Kibouris T. and Komaitis M. (2008). Essential oil composition of Pistacia lentiscus and Myrtus communis: evaluation of antioxidant capacity of methanolic extracts. Food Chem. 107, 1120-1130.
Cross D.E., Acamovic T., Deans S.G. and Cdevitt R.M. (2007). The effects of dietary inclusion of herbs and their volatile oils on the performance of growing chickens. Br. Poult. Sci. 43, 33-35.
Di Pasqua R., Betts G., Hoskins N., Edwards M., Ercolini D. and Mauriello G. (2007). Membrane toxicity of antimicrobial compounds from essential oils. J. Agric. Food Chem. 55, 4863-4870.
Garcia V., Catala-Gregori P., Hernandez F., Megias M.D. and Madrid J. (2007). Effect of formic acid and plant extracts on growth, nutrient, digestibility, intestine mucosa morphology and meat yield of broilers. J. Appl. Poult. Res. 16, 555-562.
Giannenas I., Tontis D., Tsalie E., Chronis E.F., Doukas D. and Kyriazakis I. (2010). Influence of dietary mushroom agaricus bisporus on intestinal morphology and microflora composition in broiler chickens. Res. Vet. Sci. 89, 78-84.
Iji P.A., Saki A.A. and Tivey D.R. (2001). Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Anim. Feed Sci. Technol. 89, 175-188.
Jamroz D. and Kamel C. (2002). Plant extracts enhance broiler performance. J. Anim. Sci. 80, 41-46.
Jamroz D., Wertelecki T., Houszka M. and Kamel C. (2006). Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken. J. Anim. Physiol. 90, 255-268.
Jang I.S., Ko Y.H., Kahn S.Y. and Lee C.Y. (2007). Effect of commerical essential oils on growth performance, digestive enzyme activity and intestinal microflora population in broiler ckickens. Anim. Feed Sci. Technol. 134, 304-315.
Lan P.T., Sakamoto M. and Benno Y. (2004). Effects of two probiotic Lactobacillus strains on jejunal and caecal microbiota of broiler chicken under acute heat stress condition as revealed by molecular analysis of 16S rRNA genes. Microbiol. Immunol. 8, 917-929.
Mandel L., Biswas T. and Sarkar S.K. (2000). Broilers perform well on herbs or enzymes in maize diet. World Poult. 5, 19-21.
Oviedo-Rondon E.O., Clemente-Hernandez S., Salvador F., Williams P., Losa R. and Stephen F. (2006). Essential oils on mixed coccidian vaccination and infection in broilers. Int. J. Poult. Sci. 5, 723-730.
Ozek T., Demirci B. and Baser K.H.C. (2000). Chemical composition of Turkish myrtle oil. J. Essent. Oil. Res. 12, 541-544.
Rasooli I., Moosavi M.L., Rezaee M.B. and Jaimand K. (2002). Susceptibility of microorganisms to Myrtus communis essential oil and its chemical composition. J. Agric. Sci. Technol. 4, 127-133.
Rodriguez-Vaquero M.J., Alberto M.R. and Manca De Nadra M.C. (2007). Antibacterial effect of phenolic compounds from different wines. Food Contr. 18, 93-101.
Romani A., Mulinacci N., Pinelli P., Vincieri F.F. and Tattini M. (1999). Identification and quantation of polyphenols in Myrtus communis leaves. Chromatographia. 49, 17-20.
SAS Institute. (2004). SAS^®/STAT Software, Release 8. SAS Institute, Inc., Cary, NC.
Uni Z., Smirnov A. and Sklan D. (2003). Pre and post hatch development of goblet cells in the broiler small intestine: effect of delayed access to feed. Poult. Sci. 82, 320-327.
Weyerstahi P., Marschall H. and Rustaiyan A. (1994). Constituents of the essential oil of Myrtus communis from Iran. Flavour Frag. J. 9, 333-337.
Zulkifli I., Iman Rahayu H.S., Alimon A.R., Vidyadaran M.K. and Babjee S.A. (2009). Gut microflora and intestinal morphology of commercial broiler chickens and Red Jungle Fowl fed diets containing palm kernel meal. Arch. Geflügelkd. 73, 49-55.