Isolation and Detection of Virulence factors of Salmonella typhimurium and Salmonella enteritidis

International Journal of Molecular and Clinical Microbiology

مقاله 1، دوره 2، شماره 2، بهمن 2012، صفحه 164-169   

نوع مقاله: Research Article

نویسندگان

Nazir wani؛ Rouf Ali؛ Siva Shanmugam

Department of Microbiology, M.G.R. College, Dr M.G.R. Nagar Hosur. Periyar university, Salem. Tamil Nadu, India.

چکیده

Plasmids play an important role in the virulence and antibiotic resistance of Salmonella. Salmonella carry plasmids having different size and number. Plasmid profile analysis of single isolates of Salmonella typhimurium and Salmonella enteritidis revealed the presence of 4- plasmid profiles. Salmonella serovars produce several Type III secretions including Sop-E and Sop –B. Soap E is detected by Dot-ELISA. Dot-ELISA is one of the solid phase immuneassay used to detect either antigens or antibody. Sop-B (Salmonella outer protein-B) is detected by multiplex PCR method using PCR primers Sop P1 and SOP- M13. It is concluded that the technique used in this study is more sensitive and identification and determination of various molecular characteristics of two species enhances the knowledge towards the vaccine production.

کلیدواژه‌ها

Salmonella typhimurium؛ Salmonella Enteritidis؛ salmonellosis؛ Typhoid؛ RT-PCR