Effect of Post-Mating GnRH Treatment onSerum Progesterone Profile and Conception Rate in Buffaloes

Iranian Journal of Applied Animal Science

مقاله 12، دوره 3، شماره 1، خرداد 2013، صفحه 83-90   

نوع مقاله: Research/Original Article

نویسندگان

M.Z. Lattoo^{* 1}؛ S. Prasad²؛ H.P. Gupta²؛ A. Hussain²

¹Department of Veterinary Gynecology and Obstetrics, G.B.P.U.A.T, Pantnagar, U.S, Nagar, 263145, Uttarakhand, India

²Department of Veterinary Gynecology and Obstetrics, UniversityOf Veterinary Sciences, G.B.P.U.A.T, Pantnagar, India

چکیده

The present study was designed to investigate the impact of exogenous administration of GnRH (Receptal VET^®, Intervet Pharmaceuticals Ltd., India) on serum progesterone profile and conception rates during mid luteal phase of estrous cycle in buffaloes. Estrus was induced using Cyclix 2 mL. i/m. (Intervet India Pvt. Ltd., India) and animals were bred naturally during observed estrus. The buffaloes (n=40) were grouped as control (n=10) on day 0 and treatment groups (I, II and III, n=10 in each group). GnRH (2.5 mL) was administrated on day 0, 11 and 13 of estrous cycle. Progesterone concentration was significantly higher (P<0.05; 1.782±0.046 ng/mL) in group I on day 5 and in group II on day 13 (4.514±0.038 ng/mL) and day 18 (6.173±0.015). However, in group III progesterone concentration was significantly higher on day 18 (6.554±0.0993) compared to control on day 5 (1.390±0.587 ng/mL), day 13 (3.770±0.103 ng/mL) and day 18 (5.114±0.009 ng/mL), respectively. Although progesterone concentration increased in all the three treatment groups compared to control, it was significantly higher (P<0.05) in pregnant animals of group I on days 5, 11, 12, 13 and 18 than non-pregnant animals of same group. Nevertheless, these differences were significant on days 13 and 18 in group II and on day 18 in group III in pregnant animals compared to non-pregnant animals, respectively. Similarly the conception rate was also significantly higher (P<0.05; 80% vs. 60%) in animals at group III compared to those at control.Comparison of data on progesterone profile between pregnant and non-pregnant (within group) animals of various treatment and control groups showed significantly higher levels of serum progesterone in pregnant animals on days 11, 12, 13 and 18 as compared tonon-pregnant animals of the same group. Thus, the above study revealed positive impact of GnRH administration on progesterone profile during mid luteal phase of estrous cycle, which could be used to improve fertility in buffaloes.

کلیدواژه‌ها

Buffalo؛ corpus luteum؛ estrous cycle؛ GnRH؛ hormonal response؛ progesterone

مراجع

Aboul-Ela M.B., El-Keraby F.E. and Khattab R. (1985).Effect of GnRH treatment on post-partum resumption of estrus and ovulation in buffaloes. Buffalo J. 1,61-69.
Anderson G.A. and Malmo J. (1985).Pregnancy rate of cows given synthetic gonadotropin releasing hormone at the time service. Australian Vet.J. 62,222-224.
Arikan S. and Pamukcu T. (2001). Role of oxytocin and oxytocin receptors in the synthesis of prostaglandin F2α in ruminants. Kafkas Univ. Vet. Fakult. Dergisi. 7, 123-128.
Beck N.F.G., Peters A.R. and Williams S.P. (1994). The effect of the GnRH analogue (buserelin) on day 12 post mating on reproductive performance of ewes. Anim. Prod. 58,243-247.
Beck N.F.G., Davies M.C.G. and Davies B. (1996a). A comparison of ovulation rate and late embryo mortality in ewe lambs and ewes and the role of late embryo loss in ewe lamb subfertility. Anim. Sci. 62, 79-83.
Beck N.F.G., Jones M., Davies B., Mann G.E. and Peters A.R. (1996b). The effect of the GnRH analogue (buserelin) on day 12 post mating on ovarian structure and plasma oestradiol and progesterone concentration in ewes. Anim. Sci. 63, 407-412.
Birnie L.M., Broadbent P.J. and Hutchinson J.S.M. (1997).Failure of prostaglandin F2α to induce luteolysis in GnRH agonist treated heifers. Vet. Rec. 140, 315-322.
Bullman D.C. and LammingG.E. (1978). Milk progesterone levels in relation to conception, repeat breeding and factors influencing cyclicity in dairy cows. J. Reprod. Fertil. 54, 447-458.
Busch D.C., Atkins J.A., Bader J.F., Schafer D.J., Patterson D.J., Geary T. and Wand Smith M.F. (2008).Effect of ovulatory follicle size and expression of estrus on progesterone secretion in beef cows. J. Anim. Sci. 86, 553-563.
Cam M.K., Kuran M., Yildiz S. and Selcuk E. (2002).Fetal growth and reproductive performance in ewes administered GnRH agonist on day 12 post mating. Anim. Reprod. Sci. 72, 73-82.
Campanile G., Vecchio D., Neglia G., Di Palo R., Prandi A. and D’Occhio M.J. (2008). Progesterone and pregnancy status in buffaloes treated with a GnRH agonist. Livest. Sci. 115, 242-248.
Diskin M.G. and Morris D.G. (2008). Embryonic and early foetal losses in cattle and other ruminants. Reprod. Domest. Anim. 43, 260-267.
Drew S.B. and Peters A.R. (1994). Effect of buserelin on pregnancy rates in dairy cows. Vet. Rec.134, 267-269.
Guilbaust L.A., Lussier J.G., Grasso F. and Matton P. (1990).Influence of a GnRH analogue on follicular dynamics in cows pretreated or not with FSH-P. Theriogenology. 33,240-246.
Harvey M.J.A., Renton J.P., Salaheddine M. and Robertson L. (1994). Ovarian response of cattle to Buserelin. Vet. Rec. 134, 168-171.
Humblot P. (2001). Use of pregnancy specific proteins and progesterone assays to monitor pregnancy and determine the timing, frequencies and sources of embryonic mortality in ruminants. Theriogenology. 56, 1417-1433.
Iftikhar A., Anjum R.H., Usmani M.T., Tunio A. and Abro S.H. (2009).Improvement of conception rate in crossbred cattle using GnRH analogue therapy. Pak. Vet. J. 29, 93-94.
Inskeep E.K. (2004).Preovulatory, postovulatory, and postmaternal recognition effects of concentrations of progesterone on embryonic survival in the cow. J. Anim. Sci. 82, 24-39.
Jubb T.F., Abhagoratne D., Malmo J. and Anderson G.A. (1990).Failure of an intramuscular injection of an analogue of gonadotrophin releasing hormone (Buserelin) at 11-13 days post insemination to increase pregnancy rates in dairy cattle. Australian Vet. J. 67, 359-361.
Kaim M., Blocht A., Wolfensont D., Braw-Tal R., Rosenberg M., Voet H. and Folman Y. (2003).Effects of GnRH administered to cows at the onset of estrus on timing of ovulation, endocrine responses, and conception. J. Dairy Sci. 86, 2012-2021.
Karimi A., Karami H. and Moeini M.M. (2007).The effect of administration of different levels of GnRH on the Day 0, 5 and 12 Post-Insemination on progesterone concentration in dairy heifers. Pakistan J. Biol. Sci. 10(20), 3620-3625.
Khan T.H., Beck N.F.G. and Khalid M. (2007).The effects of GnRH analogue (buserelin) or hCG (Chorulon) on day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. Anim. Reprod. Sci. 102(3-4),247-257.
Komar C.M., Berdendtson A.K., Evans A.C.O. and Fortune J. (2001).Decline in circulating estradiol during the periovulatory period is correlated with 51 decrease in estradiol and androgen, and in messenger RNA for P450 aromatase and P450 17a-hydroxylase, in bovine preovulatory follicles. Biol. Reprod. 64, 1979-1803.
Lee C.N., Maurice E., Ax R.L., Pennington J.A., Hoffman W.F. and Brown M.D. (1983). Efficacy of gonadotropin hormone administered at the time of artificial insemination of heifers and post partum repeat breeders dairy cows. American J. Vet. Rec. 44,2160-2163.
Lucy M.C. and Stevenson J.S. (1986). Gonadotropin releasing ho- rmone at estrous, luteinizing hormone, estradiol and progesterone during the periestral and post insemination period in dairy cattle. Biol. Reprod. 33,300-311.
Lynch C.O., Kenny D.A., Childs S. and Diskin M.G. (2010). The relationship between periovulatory endocrine and follicular activity on corpus luteum size, function, and subsequent embryo survival. Theriogenology. 73, 190-198.
Mac-Millan K.L., Day A.M., Taufa V.K., Gibb M. and Pearce M.G. (1985).Effects of an agonist of gonadotrophin releasing hormone in cattle 1. Hormone concentrations and cycle length. Anim. Reprod. Sci. 8, 203-212.
Mac-Millan K.L., Taufa V.K. and Day A.M. (1986).Effects of an agonist of gonadotrophin releasing hormone (Buserelin) in cattle. I. Hormone concentrations and estrous cycle length. Anim. Reprod. Sci. 1,1-10.
Mac-Millan K.L. and Thatcher W.W. (1991).Effects of an agonist of gonadotrop inreleasing hormone on ovarian follicles in cattle. Biol. Reprod. 45, 83-90.
Mandal D.D., Srivastava S.K. and Kumar P. (2004). Effect of day of GnRH administration on conception rate in buffaloes. Indian J. Anim.Sci. 74, 1189-1191.
Mandal D.D., Srivastava S.K. and Kumar P. (2009). Effect of GnRH during various stages of estrous cycle on fertility and plasma progesterone in buffaloes. Indian J. Anim.Reprod. 30, 23-27.
Mann G.E. and Lamming G.E. (1995). Effects of treatment with buserelin on plasma concentrations of oestradiol and progesterone and cycle length in the cow. British Vet. J. 151, 427-432.
Mann G.E., Lamming G.E. and Fray M.D. (1995). Plasma oestradiol and progesterone during early pregnancy in the cow and the effects of treatment with buserelin. Anim. Reprod. Sci. 37, 121-131.
Niswender G.D., Rechert L.E., Midgley A.R. and Nalbandov A.V. (1985).Radioimmunoassay for bovine and ovine luteinizing hormone. Endocrinology. 84, 1166-1173.
Perry G.A., Smith M.F., Lucy M.C., Green J.A., Parks T.E., Mac-Neil M.D., Roberts A.J. and Geary T.W. (2005). Relationship between follicle size at insemination and pregnancy success. Proc. Nation. Acad. Sci. USA. 102,5268-5273.
Perry G.A. and Perry B.L. (2009).GnRH treatment at artificial insemination in beef cattle fails to increase plasma progesterone concentrations or pregnancy rates. Theriogenology. 71, 775-779.
Rangnekar M.N., Dhoble R.L., Sawale A.G., Gacche M.G., Ingawale M.V. and Jadhav J.M. (2002). Effect of fertagyl, gonadotropin releasing hormone (GnRH),administration on conception rate in repeat breeding cows. The Blue CrossBook. 18, 20-21.
Rettmer I., Stevenson J.S. and Corah L.R. (1992a).Pregnancy rates in beef cattle after administering a GnRH agonist 11 to 14 days after insemination. J. Anim. Sci. 70, 7-11.
Rettmer I., Stevenson J.S. and Corah L.R. (1992b). Endocrine responses and ovarian changes in inseminated dairy heifers after an injection of a GnRH agonist 11 to 13 days after estrus. J.Anim. Sci. 70,508-517.
Ryan D.P., Snijders S., Condon T., Grealy M., Sreenan J. and O'Farrell K.J. (1994).Endocrine and ovarian responses and pregnancy rates in dairy cows followingthe administration of a gonadotrophin releasing hormone analogue at the timeof artificial insemination or at mid-cycle post insemination. Anim. Reprod. Sci. 34, 179-191.
Santos J.E.P., Thatcher W.W., Chebel R.C., Cerri R.L.A., Galvao K.N. (2004). The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Anim. Reprod. Sci. 82,513-535.
Saratsis P., Schimidt A.B., Vpsilantis P., Rozos C. and Demertizis A. (1998). Effect of buserelin on corpus luteum activity and the fertility of dairy cows. J. Hellenic. Vet. Med. Soc. 49, 34-38.
Selvaraj P. and Kumar H. (2001).Effect of administration of GnRH on ovulatory response, progesterone secretion and conception rate in repeat breeder cows. Indian J. Anim. Sci. 71, 938-940.
Sharma A., Singh M. and Vasishta, N.K. (2006).Effect of gonadotrophin releasing hormone administration on conception rate following artificial insemination in repeat breeder cattle. Indian J. Anim. Sci. 76, 330-332.
Singh J., Nanda A.S. and Adams G.P. (2000). The reproductive pattern and efficiency of female buffaloes. Anim. Reprod. Sci. 61,593-604.
Stevenson J.S., Phatak A.P., Rettmer I. and Stewart R.E. (1993).Postinsemination administration of receptal: Follicular dynamics, duration of cycle, hormonal responses, and pregnancy rates. J. Dairy Sci. 76, 2536-2547.
Tanabe T.Y., Dearer D.R. and Hawk H.W. (1994). Effect of gonadotrophin releasing hormone on estrus, ovulation and ovum cleavage at the time of the post-coital test. Fertil. Steril. 55, 513-515.
Thatcher W.W. and Chenault J.R. (1976).Reproductive physiological responses of cattle to exogenous prostaglandin F2α. J. Dairy Sci. 59, 1366-1375.
Thatcher W.W., Mac-Millan K.L., Hansen P.J. and Drost M. (1989).Concepts for regulation of corpus luteum function by the conceptus and ovarian follicles to improve fertility. Theriogenology. 31, 149-164.
Thatcher W.W., Drost M., MacMillan K.L., Entwistle K.W., Schmitt E.J., Dela Sota R.L. and Morris G.R. (1993). New clinical use of GnRH and its analogue in cattle. Anim. Reprod. Sci. 33, 27-49.
Ullah G., Fuquay J.W., Keawkhong T., Clark B.L., Pogue D.E. and Murphey E.J. (1996). Effect of gonadotropin-releasing hormone at estrus on subsequent luteal function and fertility in lactating Holsteins during heat stress. J. DairySci. 79, 1950-1953.
Vijayarajan A., Chandrahasan C. and Nepolean E. (2009). Efficacy of gonadotrophin releasing hormone and prostaglandin F2α in repeat breeding buffaloes. IndianVet. J. 86,368-370.
Willard S., Gandy S., Bowers S., Graves K., Elias A. and Whisnant C. (2003). The effects of GnRH administration postinsemination on serum concentrations of progesterone and pregnancy rates in dairy cattle exposed to mild summer heat stress. Theriogenology. 59, 1799-1810.
Yildiz H., Kaygusuzoglu E., Kaya M. and Cenesiz M. (2009).Effect of post-mating GnRH treatment on serum progesterone, luteinizing hormone levels, duration of estrous cycle and pregnancy rates in cows.Pak. Vet. J. 29(3), 110-114.
Yoshioka K., Suzuki C., Arai S., Iwamura S. and Hirose H. (2001). Gonadotropin releasing hormone in third ventric cerebrospinal fluid of the heifer during the estrous cycle. Biol. Reprod. 64, 563-570.
Young A.J. and Swanson L.V. (1988). Effect of GnRH and hCG at the time of insemination of repeat breeders cows. J. Dairy Sci. 71(1),137-142.
Zain A.E. and Nakao T. (1996). Pregnancy rate in repeat breeder buffalo after administration of fertelin acetate (GnRH analogue) at time of insemination or mid cycle post insemination. Pp. 221 in Proc. 2^nd ABA Congress, Philiphines.