Study of Compatibility Relationships Among Some Almond Cultivars and Genotypes Using of SAlleles Identification

Journal of Nuts

مقاله 6، دوره 05، شماره 02، اسفند 2014، صفحه 49-56   

شناسه دیجیتال (DOI): 10.22034/jon.2014.515689

نویسندگان

M. Fallah¹؛ M. Rasouli^{* 2}؛ Y. Sharaf¹؛ A. Imani³

¹Department of Horticulture, Faculty of Agriculture, Shahed University, Tehran, Iran

²Department of Horticulture and Landscape engineering, Faculty of Agriculture, Malayer University, Malayer, Iran

³Horticultural Departments of Seed and Plant Improvement Institute (SPII), Karaj, Iran

چکیده

Almond (Prunus dulcis L.) is one of the most important nut crops in Iran. Most almond cultivars and genotypes are self-incompatible. However, research on S-alleles indicates that it is very efficient in cultivar selection. Selfincompatibility in almond is gametophytic and controlled by a single S-locus with multiple codominant alleles. In this study, compatibility relationships among cultivars, “Tuono”, “Shokofeh”, “Sahand” and five improved genotypes “A1.16”, “A9.7”, “A8.39”,“A10.11” and “A230,” was investigated by the PCR of S-alleles. Degenerate primers (PaConsI-F, EMPC1consRD, EM-PC2consFD, and EM-PC3consRD) were used for amplification of S-alleles. Results showed that only “A10.11” and “A8.39” were completely cross-incompatible, but all of the other studied cultivars and genotypes were crosscompatible. Furthermore, cultivar “Tuono” and genotype “A1.16” had a self-fertility allele.

کلیدواژه‌ها

Almond؛ Degenerate primers؛ Incompatibility؛ PCR؛ S-alleles