Identification and Screening of Homozygous and Heterozygous Almond Progenies from Self-Pollinated Touno Cultivar Using PCR

Journal of Nuts

مقاله 7، دوره 06، شماره 02، بهمن 2015، صفحه 155-164   

شناسه دیجیتال (DOI): 10.22034/jon.2015.516326

نویسندگان

P. Najafi¹؛ A. Imani^{* 2}؛ S.M. Miri¹؛ M. Zinalabdini³

¹Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran

²Department of Horticultural, Seed and Plant Improvement Iinstitute (SPII), Karaj, Iran

³Department of Molecular Genomics, Agricultural Biotechnology Research Institute (ABRII), Karaj, Iran

چکیده

Self-incompatibility is one of the most important difficulties in almond production which reduce fruit set dramatically and makes orchard management difficult. Therefore, breeding almond to produce self-compatible genotypes is very important. In this research identification and screening of 86 almond progenies obtained from selfing Touno after the self-pollination by PCR reaction with specific primers of CEBAS_f and AS1. PCR results confirmed the situation of self-compatible hybrids. In addition, it indicated that, frequencies of S_f, and S₁ was 100% and 50% in progenies respectively. Self-compatible hybrids had been identified that can be used in almond breeding programs particularly to development the monoculture of almond orchards. So to identify and screening homozygous self-compatibility almonds be capable of be another step towards creating monoculture of almond and use in breeding programs further.

کلیدواژه‌ها

Almond؛ Hybrid؛ Self-compatible؛ Self-pollination