DNA interaction and antimicrobial studies of novel copper (II) complex having ternary Schiff base

Journal of the Iranian Chemical Research

مقاله 7، دوره 1، شماره 2، اسفند 2008، صفحه 129-139   

نویسندگان

Natarajan Raman^* ؛ Thanasekaran Baskaran؛ Abraham Selvan؛ Ramaraj Jeyamurugan

Research Department of Chemistry, VHNSN College, Virudhunagar 626 001, India

چکیده

A novel ternary Schiff base ligand (HL) of ONO type and its copper (II) complex were
synthesised using 2-aminophenol and o-acetoacetotoluidide. They have been characterised by the
usual analytical and spectral methods. The interaction of the complex with calf-thymus (CT)
DNA has been investigated by UV-Vis, viscosity measurement, cyclic voltammetry and
differential pulse voltammetry studies. The results show that the complex binds to CT DNA by
intercalating way. The CT DNA cleavage study was monitored by gel electrophoresis method.
From this study, it was found that the complex cleaves CT DNA in presence of the oxidant,
hydrogen peroxide. The in vitro antimicrobial activities of the synthesized compounds have been
tested against the bacteria Salmonella typhi, Staphylococcus aureus, Bacillus subtilis,
Escherichia coli, Pseudomonas putita and Klebsiella pneumoniae and fungi Aspergillus niger,
Aspergillus flavus, Rhizopus stolonifer, Candida albicans and Rhizoctonia bataicola by well
diffusion method. The Minimum Inhibitory Concentration (MIC) value against the growth of the
above microorganisms is much larger for copper complex than the ligand.

کلیدواژه‌ها

Ternary Schiff base؛ DNA binding constant؛ Intercalative binding mode؛ Redox properties؛ CT DNA cleavage؛ Antimicrobial assay

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