Radiosensitivity Study for Identifying the Lethal Dose in MR219 (Oryza sativa L. spp. Indica cv. MR219)

International Journal Of Agricultural Science, Research And Technology In Extension And Education Systems

مقاله 3، دوره 2، شماره 2، مهر 2012، صفحه 63-68   

نویسندگان

Ali Benjavad Talebi^{* 1}؛ Amin Benjavad Talebi²

¹Department of Plant Biotechnology, National institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

²Department of Computer Sciences, Faculty of Science and Information Technology, National University of Malaysia(UKM), Bangi, Malaysia

چکیده

The purpose of the research was determine the effects of gamma ray on seed germination, seedling height and root length of Rice (Oryza sativa L. spp. Indica cv. MR219) to identify the Lethal Dosage of the radiation. Paddy seeds of MR219 were exposed to different doses of gamma radiations viz 50,100,150,200,250,300,350,400,450,500,600,700,800,900 and 1000 Gy using 60Co as the radiation source. The effect of radiation was determined by measuring the Seed germination, seedling height, root length and emergence under the conditions of the M1 generation. The research results showed that the differences among radiation treatments significantly affect (p<0.01) seedling height and root length. In part of seed germination percentage, insignificant differences were observed. Also the LD25 and LD50 values observed based on the growth reduction of seedlings after treatments occurred during 250 and 450GY of exposure for the variety Oryza sativa L. spp. Indica cv. MR219. [Talebi and Talebi. Radiosensitivity Study for Identifying the Lethal Dose in MR219 (Oryza sativa L. spp. Indica cv. MR219). International Journal of Agricultural Science, Research and Technology, 2012; 2(2):63-67].

کلیدواژه‌ها

Radiosensitivity؛ Lethal Dose؛ Ionizing radiation؛ Gamma ray؛ Indica cv. MR219

مراجع

1. Abdel-Hady, M., Okasha, E., Solikan, S., and Talaat, M. (2008). Effect of gamma radiation and gibberellic acid on germination and alkaloid production in Atropa belladonna L. Australian Journal of Basic and Applied Sciences, 2(3), 401-405.

2. Ashraf, M., Cheema, A. A., M.Rashid, and Zia-ul-Qamar. (2003). Effect of gamma rays on M1 generation in basmati rice. Pakistan Journal of Botany, 35(5), 791-795.

3. Bhat, R., Upadhyaya, N., Chaudhury, A., Raghavan, C., Qiu, F., Wang, H and Till, B. (2007). Chemical-and irradiation-induced mutants and TILLING. Rice Functional Genomics, 148-180.

4. Chaomei, Z., and Yanlin, M. (1993). Radiation-induced changes in enzymes of wheat during seed germination and seedling growth. Acta Agriculture Nucleatae Sinica, 7.

5. Chaudhuri, S. K. (2002). A simple and reliable method to detect gamma irradiated lentil seeds by germination efficiency and seedling growth test. Radiation Physics and Chemistry, 64(2), 131-136.

6. Kim, J., Lee, E., Back, M., Kim, D., and Lee, Y. (2000). Influence of low dose y radiation on the physiology of germinative seed of vegetable crops. Kor J Environ Agr, 19, 58-61.

7. Kiong, A. L. P., Lai, A. G., Hussein, S., and Harun, A. R. (2008). Physiological Responses of Orthosiphon stamineus Plantles to Gamma Irradiation. American-Eurasian Journal of Sustainable Agriculture, 2(2), 135-149.

8. Kovacs, E., and Keresztes, A. (2002). Effect of gamma and UV-B/C radiation on plant cells. Micron, 33(2), 199-210.

9. Liu, L. X., Spoerke, J. M., Mulligan, E. L., Chen, J., Reardon, B., Westlund, B., . . . Hardiman, G. (1999). High-throughput isolation of Caenorhabditis elegans deletion mutants. Genome Res, 9(9), 859-867.

10. Melki, M., and Marouani, A. (2010). Effects of gamma rays irradiation on seed germination and growth of hard wheat. Environmental Chemistry Letters, 8(4), 307-310.

11. Nadeau, J. H., and Frankel, W. N. (2000). The roads from phenotypic variation to gene discovery: mutagenesis versus QTLs. Nat Genet, 25(4), 381-384.

12. Nambara, E., Keith, K., McCourt, P., and Naito, S. (1994). Isolation of an internal deletion mutant of the Arabidopsis thaliana ABI3 gene. Plant and cell physiology, 35(3), 509.

13. Preuss, S., and Britt, A. (2003). A DNA-damage-induced cell cycle checkpoint in Arabidopsis. Genetics, 164(1), 323.

14. Redei, G. P., and Koncz, C. (1992). Classical mutagenesis.

15. Shikazono, N., Tanaka, A., Watanabe, H., and Tano, S. (2001). Rearrangements of the DNA in carbon ion-induced mutants of Arabidopsis thaliana. Genetics, 157(1), 379.

16. Toker, C., Uzun, B., Canci, H., and Oncu Ceylan, F. (2005). Effects of gamma irradiation on the shoot length of< i> Cicer</i> seeds. Radiation Physics and Chemistry, 73(6), 365-367.

17. Wi, S. G., Chung, B. Y., Kim, J. S., Kim, J. H., Baek, M. H., Lee, J. W., and Kim, Y. S. (2007). Effects of gamma irradiation on morphological changes and biological responses in plants. Micron, 38(6), 553-564.

18. Wu, J. L., Wu, C., Lei, C., Baraoidan, M., Bordeos, A., Madamba, M. R.,and Leung, H. (2005). Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. [Research Support, Non-U.S. Gov't]. Plant Mol Biol, 59(1), 85-97. doi: 10.1007/s11103-004-5112-0