Optimization of Culture Conditions for Keratinase Production in Streptomyces Sp. JRS19 for Chick Feather Wastes Degradation

International Journal Of Agricultural Science, Research And Technology In Extension And Education Systems

مقاله 5، دوره 1، شماره 3، آذر 2011، صفحه 121-125   

نویسندگان

T. Jayalakshmi¹؛ P. Krishnamoorthy¹؛ G. Ramesh kumar²؛ P. Sivamani³

¹Dept of Bioinformatics, Bharath University, Chennai, Tamilnadu - 600073, India.

²Dept of Bioinformatics, MIT Campus, Anna University, Chennai, Tamilnadu-600044, India

³Microlabs, Vellore, Tamilnadu -632 521, India.

چکیده

The aim of this study was to find the optimal conditions of a newly isolated Streptomyces strain JRS 19. Screening for keratinase producing bacteria and their keratinase production were investigated. The keratinase produced by the isolate was purified by chromatographic techniques and the optimum pH maintained was 7.0 and maintained at different temperatures Viz., 20ºC, 25ºC, 30ºC, 35ºC and 40ºC. Besides temperature and pH ,the enzyme is active upon different sources and its effect on carbon, nitrogen , aminoacids were determined and recorded the protein content and enzyme activity. The carbon sources revealed that sucrose was found highly significant, whereas peptone was found highly significant in nitrogen source. The effect of keratinase on amino acid shows that valine was found highly significant. Based on this study, the physio-chemical properties of keratinase were analyzed and the results were interpreted. [T. Jayalakshmi et al. Optimization of Culture Conditions for Keratinase Production in Streptomyces Sp. JRS19 for Chick Feather Wastes Degradation. International Journal of Agricultural Science, Research and Technology, 2011; 1(3):121-125 ].

کلیدواژه‌ها

Enzyme؛ Streptomyces sp؛ feather؛ Keratinase

مراجع

1. Calik, P., Bilir, E., Calik, G And Ozdamar, T. H. (2002). Influence of pH Conditions on Metabolic Regulations in Serine Alkaline Protease Production By Bacillus Licheniformis. Enzyme And Microbial Technology, 31(5):685-697.

2. Goddard, D. R and Michaelis, M. (1934). A Study of Keratin. J.Biol Chem.106:604-614.

3. Givskov, M., Eberl, L and Molin, S. (1991).Control of Exoenzyme Production, Motility

and Cell Differentiation in Serratia Liquefaciens. Fems Microbiology Letters, 148 (2): 115-122.

4. Ignatova, Z., Gousterova, A., Spassov, G and Nedkov, P. (1998). Decomposition of Native Keratin by Thermophilic Actinomycetes. Comptes Rendus De L’ Academie Bulgare Des Sciences, 51: 67-70.

5. Ignatova,Z.,Gousterova,A.,Spassov,G.&Nedkov,P.1999 Isolation And Partial Characterization Of Extracellular Keratinase From A Wool Degrading Thermophilic Actinomycete Strain Thermo Actinomyces Candidus. Canadian Journal Of Microbiology 45,217-222.

6. Kabadjova, P., Vlahov, S., Dalgalarrondo, M., Dousset, X., Haertle, T., Briand, L and Chobert, J. M. (1996). Isolation and Distribution of Steptomyces Populations with Heat-Resistant Collagenlytic Activity from Two Protein-Rich Areas In Bulgarain Soils.Folia Microbiologica, 41:423-429.

7. Lin, X., Lee, C. G., Casale, E. S and Shih, J. C. H. (1995). Purification and Characterization of a Keratinase from a Feather Degrading Bacillus Licheniformis Strain. Applied And Environmental Microbiology, 58 (10): 3271-3275.

8. Papadopoulos, M. C. (1986). The Effect of Enzymatic Treatment on Amino Acid Content andNitrogen Characteristics of Feather Meal. Anim. Feed Sci. Technol.16:151-156.

9. Raju, A. A., Chandrababu, N. K., Samivelu, N., Rose, C and Rao, N. M. (1996). Eco-Friendly Enzymatic Dehairing Using Extracellular Proteases From A Bacillus Species Isolate. Journal Of The American Leather Chemical Association, 91 (5): 115-119.

10. Rao, M. B.,Tanksale, A. M., Ghatge, M. S and Deshpande, V. V. (1998). Molecular and Biotechnological Aspects of Microbial Proteases. Microbiology And Molecular Biology Reviews,62 (3):597-635.

11. Ryckeboer. J., Mergaert, J., Coosemans, J., Deprins, K and Swings, J. (2003). Microbiological Aspects of Biowaste during Composting In Monitored Compost Bin. Journal of Applied Microbiology, 94:127-137.

12. Suntornsuk, W and Suntornsuk, L. (2003). Feather Degradation by Bacillus Sp. Fk46 In Submerged Cultivation. Bioresource Technology,86 (3):239-243.

13. Thys, R. C. S., Lucas, F. S., Riffel, A., Heeb, P and Brandelli, A. (2004). Characterization of a Protease of a Feather-Degrading Microbacterium Species. Letters in Applied Microbiology, 39 (2): 181-186.

14. Wang, J. J and Shih , J. C. H. (1999). Fermentation Production of Keratinase from Bacillus Licheniformis Pwd-1 and a Recombinant B. Subtilis Fdb-29. Journal Of Industrial Microbiology And Biotechnology, 22 (6): 608-616.