بررسی دزهای کشنده جدایه ایرانی قارچ Beauveria bassiana در کنترل میکروبی بید آرد (Lep.: Pyralidae) Ephestia kuehniella روی

تحقیقات نوین در گیاه پزشکی

شناسنامه علمی شماره، دوره 4، شماره 1 - شماره پیاپی 13، خرداد 1391، صفحه 81-67   

نویسندگان

نگار بهمنی^* ؛ هادی استوان؛ مسعود لطیفیان؛ بهار راد

چکیده

بید آرد Ephestia kuehniella از آفات مهم انباری می­باشد. این تحقیق به ­منظور بررسی کارایی جدایه ایرانی مناسب قارچ Beauveria bassiana در کنترل میکروبی شب­پره آردEphestia kuehniella روی خرمای رقم سایر انجام شد. برای تعیین قدرت کشندگی قارچ روی مراحل تخم، لارو و شفیره، پنج دُز 10⁴،10⁴×5، 10⁵، 10⁵×5 و 10⁶اسپور در میلی­لیتر  به­روش غوطه­ورسازی استفاده شد. داده­ها با استفاده از روش لگاریتم-پروبیت برازش­گردیدند. نتایج نشان داد که بیش­ترین دُز کشنده 50 درصد مربوط به جدایه   IRAN441Cروی شفیره 10⁴ × 76/1 و کمترین دُز کشنده 50 درصد مربوط به مرحله تخم و معادل10³×49/3 اسپور در میلی­لیتر بود. بالاترین و پایین‌ترین زمان50 درصد کشندگی به­ترتیب برای شفیره و تخم و معادل  67/3 و 38/3 روز بود. به­طورکلی نتایج تحقیقات در فاز آزمایشگاهی که قدم نخست در امکان‌سنجی کاربرد این قارچ در کنترل میکروبی بید آرد می­باشد، نشان داد که قارچ عامل بیمارگر از پتانسیل کاربرد بالایی برخوردار است.

کلیدواژه‌ها

کنترل میکروبی؛ بید آرد؛ خرما

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