Isolation of L-Glutaminase Producing Marine Actinomycetes

International Journal of Molecular and Clinical Microbiology

مقاله 7، دوره 6، شماره 1، مهر 2016، صفحه 635-642   

نوع مقاله: Research Article

نویسنده

Periasamy Dhevagi^*

Tamil Nadu Agricultural University

چکیده

L Glutaminase, a therapeutic enzyme obtained from marine Actinomycetes gains importance because of their adaptability to varied environmental factors. An experimental study was carried out to isolate the L glutaminase producing actinomycetes from the marine sediment of Thoothukudi. Marine sediment sample was collected from six different locations of the Thoothukudi coastal ecosystem, enriched with glutamine broth to enhance the population of Actinomycetes. After enrichment , selective media were used for isolating the Actinomycetes. Ninety four isolate was obtained and all the isolates were screened for L Glutaminase activity in a rapid plate assay method. Three isolates showed strong activity with enzyme production to the level of 9.42 IU to 16.94 IU /ml. The isolate DSG 18 had produced 16.94± 0.62 IU and it could be used a good source of glutaminase.

کلیدواژه‌ها

Actinomycetes؛ Marine Ecosystem؛ Therapeutic Enzymes؛ Enzyme purification؛ anticancerous activity