Increasing the Vase Life of Alstroemeria Cut Flowers Using Cycloheximide, Benzyladenine and Coconut Juice

Journal of Ornamental Plants

مقاله 2، دوره 6، شماره 4، اسفند 2016، صفحه 225-236   

نوع مقاله: Research Paper

نویسندگان

Saeedeh Alizade Matak؛ Davood Hashemabadi^*

Department of Horticulture, Rasht Branch, Islamic Azad University, Rasht, Iran

چکیده

In order to improve the vase life of alstroemeria cut flowers (Alstroemeria aurantiaca), an experiment was performed based on completely randomized design with 10 treatments including cycloheximide (50, 100 and 200 mg l^-1), benzyladenine (50, 100 and 200 mg l^-1), coconut juice (5, 10 and 20 %) and control (distilled water) in three replications. In this study, characteristics such as vase life, dry matter, increasing ° Brix, the percentage of flower opening, chlorophyll a and b and ionic leakage were evaluated. The results showed that coconut juice at a concentration of 5% has the maximum vase life (17.39 days), although it has no significant difference with different levels of benzyladenine and 10 and 20% coconut juice treatments. 10% coconut juice with 2.58 and 0.88 has the highest ° Brix and flower opening index, respectively. Treatments of 50 mg l^-1 benzyladenine, 5 and 10% coconut juice had the maximum impact on the amount of dry matter. The most successful treatments for reducing ionic leakage are two treatments of 5% coconut juice and 100 mg l^-1 benzyladenine. All treatments cause maintenance of chlorophyll a and b compared to the control, and two treatments of 50 mg l^-1 cycloheximide and 5% coconut juice had the highest content of chlorophyll a and b, respectively among treatments. According to the obtained results, using 5% coconut juice in the vase solution can effectively improve the vase life of alstroemeria cut flowers.

کلیدواژه‌ها

Benzyladenine؛ Chlorophyll؛ Cytokinin؛ Ionic leakage؛ Vase life

مراجع

Abd El- Aziz, N.G. 2007. Stimulatory effct of NPK fertilizer and benzyladenin on growth and chemical constituents of Codiaem variegatum L. plant. American-Eurasian Journal of Agricultural and Environmental Sciences, 2(6): 711-719.

 Agampodi, V.A. and Jayawardena, B.M. 2007. Effect of coconut water in extending the vase life of Anthurium cut flower variety Wild Pink. Tropical Agricultural Research, 19: 202-209.

 Arefnia, R., Hatamzadeh, A., Ghasemnezhad, M., Javdani, Z., Ghamgosar, R. and Arabzadeh, A. 2013. Effect of benzyladenin on carotenoid content and vase life Lilium oreintal cv. Belladona. 8^th Congress of Iranian Horticultural Science. 26 August 2013, Bu-Ali Sina University, Iran, Hamedan. 2228-2230.

 Bieleski, R.L. and Reid, M.S. 1991. Physiological changes accompanying senescence in the ephemeral daylily flower. Plant Physiology, 98: 1042–1049.

Chanasut, U., Rogers, H.J., Leverenttz, M.K., Griffiths, G., Thomas, B., Wagstaff, C. and Stead, A.D. 2003. Increasing flower longevity in Alstroemeria. Postharvest Biology and Technology, 29: 324-332.

 Dai, J.W. and Paull, R.E. 1991. Postharvest handling of Alstroemeria. Hort Science, 26: 314.

Davis, P.J. 1998. Plant hormones and their role in plant growth and development. Kluwer Academic Publishers. 432 page.

 Drory, A., Beja Tal, S., Borochov, A., Gindin, E. and Mayak, S. 1995. Transient water stress in cut carnation flowers: effects of cycloheximide. Scientia Horticulturae, 64(3): 167-175.

 Dudareva, N., Auria, J.C.D., Nam, K.H., Raguso, R.A. and Pichersky, E. 1998. Acetyl CoA: benzyl alcohol acetyl transferase: an enzyme involved in floral scent production in Clarkia breweri. Plant Journal, 14:297-304.

 Ferrant, A., Hunter, D.A., Hackett, W.P. and Reid, M. 2002. Thidiazuron- a potent inhibitor of leaf senescence in Alestromeria. Postharvest Biology and Technology, 25: 333-338.

 Gilbart, D.A. and Dedolph, R.R. 1965. Phytokinins effects on respiration and photosynthesis in roses and broccoli. American Society for Horticultural Science, 86: 774-778.

 Gul, F. and Tahir, L. 2013. An effective protocol for improving vase life and postharvest performance of cut Narcissus tazetta flowers. Journal of the Saudi Society of Agricultural Sciences, 12: 39–46.

 Gulzar, S., Tahir, I., Farooq, S. and Sultan, S.M. 2005. Effect of cytokinins on the senescence and longevity of isolated flowers of daylily (Hemerocallis fulva cv. "Royal Crown") sprared with cycloheximide. Acta Horticulturae, 669: 395-403.

 Halaba, J. and Rudnicki, R.M. 1986. The role of enzymes during senescence of cut flowers. Acta Horticulturae, 181: 65-74.

 Halevy, A.H. and Mayak, S. 1981. Senescence and postharvest physiology of cut flowers. Part 2. Horticultural Reviews, 3: 59-143.

 Han, S.S. and Miller, J.A. 2003. Role of ethylene in postharvest quality of cut oriental lily ‘Stargazer’. Plant Growth Regulation, 40: 213-222.

 Hassanpour Asil, M. and Karimi, M. 2010. Efficiency of benzyladenine reduced ethylene production and extended vase life of cut Eustoma flowers. Plant Omics Journal, 3(6): 199-203.

 Hichlenton, P.R. 1991. GA₃ and benzylaminopourine delay leaf yellowing in cut Alstroemeria stems. Horticultural Science, 26(9): 1198-1199.

 Huang, K.L. and Chen, W.S. 2002. BA and sucrose increase vase life of cut Eustoma flowers. Horticultural Science,37: 547–549.

 Hunter, D.R., Norbery, C.L. and Segol, I.H. 1973. Effect of cycloheximide on l-Leucine transport by Penicillium chrysogenum: Involvement of calcium. Journal of Bacteriology, 114: 956-960.

 Islam, S.T., Tahir, I., Waseen, S. and Bhat, M.A. 2011. Effect of cycloheximide on senescence and postharvest performance in Hemerocallis fulva cv. "Royal Crown". Plant Science, 6(11): 14-25.

 Jayalekshmy, A., Arumughan, C., Narayana, C. and Mathew, A.G. 1986. Changes in the chemical composition of coconut water during maturation. Journal of Food Science and Technology, 23(5): 203-207.

Jones, R.B., Serek, M., Kuo, C.L. and Reid, M.S. 1994. The effect of protein synthesis inhibition on petal senescence in cut bulb flowers. American Society for Horticultural Science, 119: 1243-1247.

 Kaya, C., Higges, D. and Kirnak, H. 2001. The effects of high salinity (NaCl) and  supplementary phosphorus and potassium  on physiology and nutrition development  of spinach. Journal of Plant Physiology, 27 (3-4): 47-59.

 Kazemi, M., Zamani, S. and Aran, M. 2011. Effect of some trearment chemicals on keeping quality and vase life of gerbera cut flowers. American Journal of Plant Physiology, 2: 99-105.

 Kioshi, O. 2003. Effects of gebberelline and benzyl adenine on dormancy and flowering of Lilium speciosum. Kanagawa Horticultural Experimental Station, Ninomiya, Nakagun, Kanagawa, 259-261.

Kumar, N., Srivastava, G.C. and Dixit, K. 2008. Flower bud opening and senescence in roses. Plant Growth Regulation, 55: 81-99.

 Lay-yee, M., Stead, A.D. and Reid, M.S. 1992. Flower senescence in daylily (Hemerocallis). Physiologia Plantarum, 86: 308–314.

Lesham, Y.Y. 1988. Plant senescence processes and free radicals. Free Kadical Biology and Medicine, 5: 39-49.

 Lukaszewski, T.A. and Reid, M.S. 1989. Bulb type flower senescence. Acta Horticulturae. 261: 59–62.

 Mamaril, J.C., Trinidad, L.C. and Paner, E.T. 1986. Methods of extraction of plant growth hormoes in coconut water: I. UV characterization. National Academy of Science and Technology, 8: 225-238.

 Mazumdar, B.C. and Majumder, K. 2003. Methods on physcochemical analysis of fruits. University College of Agriculture, Calcutta University. 136-150.

 Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7: 405-410.

Mortazavi, n., Naderi, R., Khalighi, A., Babalar, M. and Alizadeh, H. 2007. The effect of cytokinin and calcium on cut flower quality in rose (Rosa hybrid L.) cv Illona. Journal of Food Agriculture and Enviroment, 5 (3-4): 311-313.

 Mutui, T.M., Emongor, V.E. and Hutchinson, M.J. 2001. Effect of accel on the vase life and postharvest quality of alstroemeria (Alstroemeria aurantica L.) cut flowers. African Journal of Science and Technology, 2: 82-88.

 Mutui, M .T., Emongor E. V. and Hutchinson, M.J. 2004. Effect of benzyladenine on the vase life and keeping quality of alstroemeria cut flowers. Journal of Agriculture Science. 5:91-105.

 Nair, S.A., Sirasamy, A., Attri, B.L. and Sharma, T.V.R.S. 2000. Effect of natural and chemical floral preservatives on vase life of cut Gerbera, a comparative study.  Indian Coconut Journal, 31(3): 29-31.

 Ojaghi, J., Mortazavi, S.N. and Bagheri Tirtashi, Z. 2013. Effect of spraying benzyladenin in preharvest on longvity and quality of alstroemeria cut flower (cv. Fuji). 8^th Congress of Iranian Horticultural Science. 26 August 2013, Bu-Ali Sina University, Iran, Hamedan. 2294-2298.

 Penner, D. and Wiely, L. 2008. The influence of atrazine, benzyladenine and the embryonic axis on chlorophyll synthesis in cucumber and squash cotyledons. Physiologia Plantarum, 26(1): 148-151.

 Salisbury, F.B. and Ross, C.W. 1986. Plant physiology. Belmont, California Wadsworth Publishing Company, pp. 319-329.

 Shahri, W. and Tahir, I. 2010. Effect of cycloheximide on senescence and postharvest performance of Ranunuculus asiaticus flowers. Pakistan Journal of Botany, 42(5): 3577-3585.

 Singh, A., Kumar, J. and Kumar, P. 2008. Effects of plant growth requlators and sucrose on postharvest physiology membrane stability physiology and vase life of cut spike of gladiolus. Plant Growth Regulation, 55: 221-229.

 Suttle, J.C. and Kende, H. 1980. Ethylene action and loss of membrane integrity during petal senescence in Tradescantia. Plant Physiology, 65: 1067-1072.

 Thimann, K.V. 1987. Plant scenescence: A proposed integration of the constitiuent processes page 1-19. In: Thomson, W.W., Northnegel, E.A. and  Huffaker, R.C. (eds) plant scenescense. Its biochemistry and physiology. American Society of Plant Physiologists, Rockvills Md.

 Van Doorn, W.G., Harmannus, H. and Song, J.S. 1994. Water relations and scenescence of cut Iris flowers: effects of cycloheximide. Postharvest Biology and Technology, 5: 345-351.

Wulster, G., Sacalis, J. and Janes, H. 1982. The effect of inhibitors of protein synthesis on ethylene induced senescence in isolated carnation petals. American Society for Horticultural Science, 107: 112–115.