Detection of Novel Mutation in LukS Panton-Valentine Leukocidin Gene in Twelve Isolates of Staphylococcus aureus from Sudanese Patients

International Journal of Molecular and Clinical Microbiology

مقاله 1، دوره 6، شماره 2، اسفند 2016، صفحه 663-669   

نوع مقاله: Research Article

نویسندگان

Najem Aldin Mohammed Osman^{* 1}؛ Intisar Elhag Elrayah²؛ Hisham Altayb³؛ Nihad Mohammed Elhaj⁴؛ Mohamed Ahmed Salih⁵؛ Nadir Abuzeid⁶؛ Muataz Mohmed Eldirdery⁴؛ Yassir Mahgoub Mohamed⁴

¹Department of Biotechnology, Faculty of Science and Technology, Omdurman Islamic University, Sudan

²College of Applied Medical Science, Shaqra University, KSA

³Department of Microbiology, College of Medical Laboratory Sciences, Sudan University for Science and Technology

⁴Department of Microbiology, Tropical Medicine Research Institute, National Center for Research, Sudan

⁵Department of Biotechnology, Biotechnology Park, Africa City of Technology, Sudan

⁶Faculty of Medical Laboratory Sciences, Omdurman Islamic University

چکیده

Staphylococcus aureus carrying PVL gene remain major health problem associated with highly virulent infections. Characterization of such gene is important to know the impact and the functional significance of nucleotide variations. PCR and standard sequencing were performed for twelve Sudanese strains from different sources. Protein structures prediction, modeling and physiochemical analysis were done to analyze genetic variation in PVL. Single nucleotide polymorphism at position 610 (A / G) of lukS-PV was detected in all tested isolates resulted in nonsynonymous mutation in the amino acid sequence at position 204 (N / D). Using molecular and bioinformatics tools the detected novel mutation in lukS-PV may have a diagnostic functional significance.

کلیدواژه‌ها

S. aureus؛ PVL؛ lukS-PV؛ Mutation؛ Sudanese