Detection of Human Papillomavirus in Osophageal Squamous Cell Carcinoma samples in Mazandaran Province, Iran

International Journal of Molecular and Clinical Microbiology

مقاله 1، دوره 7، شماره 1، شهریور 2017، صفحه 741-747   

نوع مقاله: Research Article

نویسندگان

Mahtab Alijanpour Aghamolkei¹؛ Alijan Tabarraei^{* 2}؛ Abdolvahab Moradi¹؛ Farzin sadeghi³؛ Yousef Yahyapour^{* 4}

¹Department of Medical Virology, Golestan University of Medical Sciences, Gorgan, Iran

²infectious diseases research center, Golestan University of Medical Sciences, Gorgan, Iran

³Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran

⁴Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran

چکیده

We examined 170 formalin-fixed paraffin-embedded samples from esophageal squamous cell carcinoma patients. All subjects live in Mazandaran province, a region with high incidence rate of esophageal cancer and have become known as the “Asian Esophageal Cancer Belt”. Samples were tested for HPV-DNA by MY09/11 and Gp5+/6+ general primers using nested PCR. Of the 170 ESCC samples, 86 (50.6%) were male and 84 (49.4%) were female. The mean age of the subjects was 66.5±11.1 and ranged from 35 to 91 years. Totally, HPV-DNA was detected in 62 (36.5%) of the esophageal squamous cell carcinoma samples by HPV L1 consensus primers. Considering the location of esophagus specimens, of 62 positive samples, 16 (25.8%) samples were in the upper third, 28 (45.2%) in the middle third, and 18(29.0%) in the lower third.  The current study showed a relatively substantial prevalence of HPV infection in esophageal squamous cell carcinoma samples in Mazandaran province.

کلیدواژه‌ها

HPV؛ Nested PCR؛ Osophageal Cancer؛ Mazandaran؛ Iran