Resistance of various biotypes of Canary grass (phalaris. Spp) to acetyl-CoA carboxylase-inhibiting herbicides.

Iranian Journal of Plant Physiology

مقاله 3، دوره 7، شماره 1، دی 2016، صفحه 1899-1907   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2016.532429

نویسندگان

Rahil Abdi^{* 1}؛ Eskandar Zand²؛ Mohammad Reza Naghavi³؛ Jahanfar Daneshiyan²؛ Nour Ali Ghiasi⁴

¹Department of Weed Science,Faculty of Agriculture, Islamic Azad University,Takestan Branch, Takestan, Iran 2

²Department of Weed Research,Iranian Plant Protection Research Institute, Iran

³Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Iran

⁴Department of Agronomy and Plant breeding, Faculty of Agriculture,University of Zanjan, Iran

چکیده

Little seed canary grass (Phalaris minor L.) is a major weed in wheat fields in some parts of Iran. To evaluate the efficacy of molecular and greenhouse methods in detecting the resistance of 49 biotypes of canary grass(Phalaris. Spp) to acetyl-CoA carboxylase-inhibiting herbicides, two methods including whole plant screening and PCR-based molecular methods were applied. Results showed that there were resistant biotypes (ile-1781-Leu) among the studied weed populationand the similarity between greenhouse and molecular methods was 67%.  According to the molecular method, an isoleucine (ile) 1781 to leucine (leu) mutation in plastidicACCase enzyme of 30 biotypes (67% of biotypes) was identifiedas a mutation endowing to the clodinafop-propargyl resistance. The partial differences  of about 33% between greenhouse and molecular methods can be explained by mutation in another location or through another metabolism –based mechanism.

کلیدواژه‌ها

ACCase inhibitors؛ Resistance؛ Phalaris sp؛ whole plant assay؛ PCR