تاثیر قارچ اندوفیت Piriformospora indica بر بهبود شاخص های رشد و فعالیت آنزیم های آنتی اکسیدان در گیاه برنج(Oryza sativa L.) تحت تنش شوری

زیست شناسی تکوینی

مقاله 5، دوره 9، شماره 2 - شماره پیاپی 34، خرداد 1396، صفحه 57-71   

نوع مقاله: مقاله پژوهشی

نویسندگان

علی اصغر باقری کشتلی^* ؛ فریبا خسروی نژاد

گروه زیست شناسی، واحد رودهن، دانشگاه آزاد اسلامی، رودهن، ایران

چکیده

تنش های محیطی از جمله شوری، مهمترین عوامل محدود کننده رشد و عملکرد گیاهان زراعی در مناطق خشک و نیمه
خشک هستند. قارچ اندوفیت Piriformospora indica دارای خاصیت تحریک رشد گیاه و افزایش مقاومت آن به
تنشهای محیطی، از جمله شوری، خشکی و بیماریهای گیاهی میباشد. در این پژوهش توان قارچ اندوفیت P. indica
در بهبود پارامترهای رشد و افزایش مقاومت به تنش شوری گیاه برنج (Oryza sativa L.) با تکیه بر افزایش فعالیت
آنزیمهای آنتی اکسیدان مورد مطالعه قرار میگیرد . بدین منظور، آزمایش گلخانه ای در قالب طرح بلوکهای کامل
تصادفی با 3 تکرار و با دو تیمار شامل 2 سطح قارچ تلقیح شده و تلقیح نشده )شاهد( و 4 سطح شوری 0 ، 100 ، 200 و
300 میلی مولار نمک ) NaCl ( انجام گرفت. نتایج حاصل از این پژوهش نشان دادکه قارچ P. indica سبب افزایش
پارامترهائی رشدی مانند بیومس )زیتوده( اندامهای هوایی و ریشه، محتوای نسبی آب، محتوای پرولین و فعالیتهای
آنزیم های آنتی اکسیدانی چون گلوتاتیون ردوکتاز، آسکوربات پراکسیداز، کاتالاز و سوپر اکسید دیسموتاز و کاهش تولید
مالون دی آلدئید ) حاصل از پراکسیداسیون لیپیدهای غشائی ( گیاهان تلقیح شده نسبت به گیاهان شاهد در تمام سطوح
شوری شد. بنابراین میتوان نتیجه گرفت که قارچ اندوفیت P.indica ب ه لحاظ بهبود سیستم دفاعی آنتیاکسیدانی
خطرات ناشی از اثر تنش شوری بر رشد گیاه برنج را تعدیل مینماید.

کلیدواژه‌ها

آنزیمهای آنتیاکسیدان؛ برنج ) Oriza sativa (؛ تنش شوری؛ Piriformospora indica

سایر فایل های مرتبط با مقاله

Abstract-maghaleh-5.pdf

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