Microextraction and Determination of Diclofenac in Biological Samples using Hollow Fiber Liquid Phase Microextraction Technique Coupled with HPLC-UV

Journal of Applied Chemical Research

مقاله 2، دوره 12، شماره 1، فروردین 2018، صفحه 16-25   

نوع مقاله: Research Paper

نویسندگان

Mohammad Reza Saadat¹؛ Mahnaz Qomi^{* 2}؛ Sahel Emadzadeh¹؛ Marjan Gholghasemi³

¹Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch , Islamic Azad University, Tehran, Iran

²2,4Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

³Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran

چکیده

In this study, hollow fiber liquid phase microextraction (HF-LPME) coupled with highperformance
liquid chromatography (HPLC) with UV detection was applied for preconcentration
and determination of Diclofenac sodium in biological fluids. Parameters affecting the extraction
process including pH of donor phase and acceptor phase, type of extraction solvent, stirring rate,
extraction time, and salt addition were studied and optimized. Under the optimized conditions
donor phase pH=3, acceptor phase pH=11.7, extraction solvent n-octanol, stirring rate =750 rpm,
extraction time=27 min and without the addition of salt, enrichment factors up to 170 were
achieved and the relative standard deviation (RSD %) of the method was within the range of 2-
3%. The method was successfully applied to determine the concentration of diclofenac sodium in
the biological samples and satisfactory results were obtained.

کلیدواژه‌ها

Diclofenac؛ Liquid-phase microextraction؛ HPLC؛ Biological samples