A Preliminary Experiment on Agrobacterium tumefaciens-Mediated Transformation of the P5CS1 GENE in Tall Fescue

Journal of Ornamental Plants

مقاله 1، دوره 8، شماره 2، شهریور 2018، صفحه 79-86   

نوع مقاله: Research Paper

نویسندگان

Mostafa K. Sarmast^{* 1}؛ Hassan Salehi²؛ Mehrnaz Zarei²

¹Department of Horticultural Science, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources (GUASNR), Gorgan 49138-43464, Golestan, Iran

²Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran

چکیده

Abiotic stress conditions can be imposed on plants through either poor water quality or adverse climate condition. These types of stress affect plant growth and development throughout their life cycle. Plants that are tolerant to drought are therefore valuable, and turf grasses have the potential to grow tolerant. Turf grasses provide ground cover and offer multiple benefits, from their aesthetic value which affects our daily lives to their preservation of land against erosion. The objectives of the present work were to find the ideal media for callus induction and regeneration followed by overexpressing the P5CS1 gene encoding proline for improving drought stress resistance in Festuca arundinacea Schreb. The husks of tall fescue seeds were removed and the seeds were then sliced longitudinally. The result of this treatment showed a greater callus induction efficiency in comparison with intact seeds in the culture media. In addition, an increase in regeneration efficiency was observed in media supplemented with 2,4-D along with BAP. The average of G418‒resistant calli obtained in the experiment was around 10%. The heterologous transformation of P5CS1 in F. arundinacea background was confirmed by PCR and the transient Gus assay. More than 90% of calli expressed the uida gene which can most probably convey resistance to drought stress.

کلیدواژه‌ها

Drought stress؛ <i>F. arundinacea</i>؛ Gus assay؛ Uida gene

مراجع

Barnes, R.F. 1990. Importance and problems of tall fescue. In: Kasperbauer, M.J. (ed.) Biotechnology in Tall Fescue Improvement. CRC Press, Boca Raton, pp. 1–12.

Bettany, A.J.E., Dalton, S.J.,  Timms, E., Manderyck, B., Dhanoa, M.S. and Morris P. 2003. Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.). Plant Cell Report, 21:437–444.

Choudhary, N.L., Sairam, R.K. and Tyagi, A. 2005. Expression of delta1-pyrroline-5-carboxylate synthetase gene during drought in rice (Oryza sativa L.). Indian Journal of Biochemistry and Biophysics, 42: 366–370.

Cougnon, M., Baert, J., Van Waes, C. and Reheul, D. 2013. Performance and quality of tall fescue (Festuca arundinacea Schreb.) and perennial ryegrass (Lolium perenne L.) and mixtures of both species grown with or without white clover (Trifolium repens L.) under cutting management. Grass and Forage Science. 69: 666–677.

Delauney, A.J. and Verma, D.P.S. 1993. Proline biosynthesis and osmoregulation in plants. Plant Journal, 4: 215-223.

Dong, S. and Qu, R. 2005. High efficiency transformation of tall fescue with Agrobacterium tumefaciens. Plant Science 168: 1453–1458.

Fariaszewska, A., Aper, J., Huylenbroeck, J. V., Baert, J. and Riek, J.D. 2016. Mild drought stress-induced changes in yield, physiological processes and chemical composition in Festuca, Lolium and Festulolium. Journal of Agronomy and Crop Science, 203: 103-116.

Foyer, C.H., Descourvierse, P. and Kunert, K.J. 1994. Protection against oxygen radicals: an important defense mechanism studied in transgenic plants. Plant Cell and Environment, 17: 507–23.

Gao, C., Long, D., Lenk, I. and Nielsen, K.K. 2008. Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment. Plant Cell Reports, 27: 1601–1609.

Ha, S.B., Wu, F.S. and Thorne, T.K. 1992. Transgenic turf-type tall fescue (Festuca arundinacea Schreb.) plants regenerated from protoplasts. Plant Cell Reports, 11: 601– 604.

Haudecoeur, E., Planamente, S.,  Ciroum A.,  Tannièresm M.,   Shelp, B.J.,  Moréra, S. and  Faure, D. 2009. Proline antagonizes GABA-induced quenching of quorum-sensing in Agrobacterium tumefaciens. PNAS, 106: 14587–14592.

Hu, C.A.A., Delauney, A.J. and Verma, D.P.S. 1992. A bifunctional enzyme (Δ¹-pyrroline-5-carboxylate synthetase) catalyzes the first two steps in proline biosynthesis in plants. PNAS, 89: 9354-9358.

Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reports, 5:387–405.

Kumar, V., Shriram, V., Kavi Kishor, P.B., Jawali, M. and Shitole, M.G. 2010. Enhanced proline accumulation and salt stress tolerance of transgenic indica rice by over-expressing P5CSF129A gene. Plant Biotechnology Reports, 4:37–48.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum, 15:473-497.

Pawłowicz, I., Rapacz, M., Perlikowski, D., Gondek, K. and Kosmala, A. 2017. Abiotic stresses influence the transcript abundance of PIP and TIP aquaporins in Festuca species. Journal of Genetics, 58:421-435.

 Saradhi, P., Alia, P., Arora, S. and Prasad, K.V. 1995. Proline accumulates in plants exposed to UV radiation and protects them against UV induced peroxidation. Biochemistry Biophysics Research Communication, 209:1–5.

Sarmast, M.K. 2016. Genetic transformation and somaclonal variation in conifers- a review. Plant Biotechnology Reports, 10: 309-325.

Sarmast, M.K., Salehi, H. and Niazi, A. 2015. Biochemical differences underlie varying drought tolerance in four Festuca arundinacea Schreb. genotypes subjected to short water scarcity. Acta Physiologia Plantarum, 37: 192

Savouré, A., Jaoua, S., Hua, X.J., Ardiles, W., van Montagu, M. and Verbruggen, N. 1995. Isolation, characterization, and chromosomal location of a gene encoding the delta 1-pyrroline-5-carboxylate synthetase in Arabidopsis thaliana. FEBS Letter, 372: 13–19.

Schat, H. 1997. Heavy metal-induced accumulation of free proline in a metal-tolerant and a nontolerant ecotype of Silene vulgaris. Physiologia Plantarum,101: 477–482.

Siripornadulsil, S., Traina, S., Verma, D.P.S. and Sayre, R.T.S. 2002. Molecular mechanisms of proline-mediated tolerance to toxic heavy metals in transgenic microalgae. The Plant Cell, 14: 2837–2847.

Szabados, L. and Savoure, A. 2009. Proline: A multifunctional amino acid cell. Trends in Plant Science, 15: 89-97

Verbruggen, N. and Hermans, C. 2008. Proline accumulation in plants: A review. Amino Acids, 35: 753–759.

Yang, Z., Miao, Y., Yu, J., Liu, J. and Huang, B. 2014. Differential growth and physiological responses to heat stress between two annual and two perennial cool-season turfgrasses. Scientia Horticulturea, 170: 75–81.

Yoshiba, Y., Kiyosue, T., Katagiri, T., Ueda, H., Mizoguchi, T., Yamaguchi-Shinozaki, K., Wada, K., Harada, Y. and Shinozaki, K. 1995. Correlation between the induction of a gene for Δ¹-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress. Plant Journal, 7: 751-760.

Zhao, J., Zhi, D., Xue, Z., Liu, H. and Xia, G. 2007. Enhanced salt tolerance of transgenic progeny of tall fescue (Festuca arundinacea) expressing a vacuolar Na+/H+ antiporter gene from Arabidopsis. Journal of Plant Physiology, 164: 1377-1383.

Zhuang, L., Wang, J. and Huang, B. 2017.  Drought inhibition of tillering in Festuca arundinacea associated with axillary bud development and strigolactone signaling. Environmental and Experimental Botany, 142: 15–23.