بررسی اثر میکوریزGlomus mosseae و براسینواستروئید بر مکانیسم فتوسنتز آنیسون (Pimpinella anisum L.) تحت شرایط تنش کادمیوم

زیست شناسی تکوینی

مقاله 5، دوره 10، شماره 3 - شماره پیاپی 39، شهریور 1397، صفحه 55-71   

نوع مقاله: مقاله پژوهشی

نویسندگان

سپیده حاج باقری¹؛ حسین عباسپور^{* 1}؛ شکوفه انتشاری²؛ علیرضا ایرانبخش³

¹گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد دامغان، دامغان، ایران

²گروه زیست شناسی، دانشگاه پیام نور، ایران

³گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران

چکیده

عناصر سنگین از مهمترین آلاینده‌های محیطی هستند و سمیت آنها به ‌دلایل اکولوژیکی، تکاملی، تغذیه‌ای و محیطی مشکل بزرگی به‌شمار می‌رود. بسیاری از تحقیقات نشان داده اند که تلقیح گیاهان با قارچ میکوریزی یا استفاده از هورمون براسینواستروئید مقاومت بسیاری از گیاهان را نسبت به فلزات سنگین افزایش می‌دهد. در این مطالعه تاثیر میکوریز Glomus mosseae و 24-اپی‌براسینولید (10-6 میکرومولار) بر مکانیسم فتوسنتز و مقاومت گیاه دارویی آنیسون نسبت به تنش ناشی از کلرید کادمیوم (0، 100، 200 و 800 ppm) مورد بررسی و مقایسه قرار گرفت. نتایج نشان داد که کادمیوم باعث کاهش درصد آغشتگی میکوریزی ریشه، کلروفیل a، کلروفیل b و کلروفیل کل و کاهش حدواسط‌های مسیر بیوسنتز کلروفیل شامل پروتوپورفیرین IX، منیزیم پروتوپورفیرین IX، پروتوکلروفیلید، کلروفیلید a و کلروفیلیدb و کاهش کاروتنوئید در گیاه آنیسون شد. پیش تیمار گیاهان با براسینواستروئید، تلقیح گیاهان با قارچ میکوریز Glomus mosseae و اثر توام Glomus mosseae×براسینواستروئید باعث افزایش میزان این ترکیبات تحت غلظت‌های 100 و 200 ppm کلرید کادمیوم گردید. بنابراین می توان نتیجه گرفت که براسینواسترویید و قارچ میکوریزی در این غلظت‌ها بر مکانیسم فتوسنتز و مقاومت در این گیاه نقش مثبت داشته و باعث مقاومت این گیاه در برابر سمیت ناشی از کادمیوم در این گیاه می‌شود.

کلیدواژه‌ها

آنیسون؛ براسینواستروئید؛ کلرید کادمیوم؛ میکوریز Glomus mosseae

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Abstract-maghaleh-5.pdf

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