بررسی اثر عصاره گیاه کرفس (Apium graveolens) بر میزان کلسیم و اگزالات سرم در آسیب کلیوی ناشی از اتیلن ‌گلیکول در موش‌های صحرایی نر بالغ نژاد ویستار

آسیب‌شناسی درمانگاهی دامپزشکی

مقاله 4، دوره 12، 2 (46) تابستان، شهریور 1397، صفحه 133-144   

نوع مقاله: مقاله علمی پژوهشی

نویسندگان

پریسا مهرپرور¹؛ اکرم عیدی^{* 1}؛ سید پژمان مرتضوی²؛ شهربانو عریان¹

¹گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

²دانشیار پاتولوژی، گروه پاتولوژی دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

چکیده

کرفس (Apium graveolens) گیاهی دوساله و از خانواده Apiaceae است. بخش­های مختلف کرفس در اختلالات کبدی و طحال، اختلالات مغزی، درد و اختلالات خواب استفاده می­شود. مطالعه حاضر به منظور بررسی اثر عصاره اتانولی کرفس بر آسیب کلیه ناشی از اتیلن­ گلیکول در موش­های صحرایی نر نژاد ویستار انجام گرفت.القاء سنگ­کلیه با استفاده از تیمار 75/0 درصد اتیلن­ گلیکول به مدت 28 روز انجام شد. موش­ها به 8 گروه 6تایی تقسیم شدند که به ترتیب شامل کنترل سالم، کنترل سنگ کلیوی (75/0 درصد اتیلن­ گلیکول در آب آشامیدنی)، حیوانات سالم تیمار با عصاره کرفس در دوزهای 50، 100 و 200 میلی­گرم بر کیلوگرم و موش­های سنگ کلیویتیمار با عصاره کرفس در دوزهای 50، 100 و 200 میلی­گرم در کیلوگرم وزن بدن به صورت گاواژ بودند. در پایان آزمایش (28 روز)، نمونه خون از قلب موش­ها جمع­آوری شد و میزان کلسیم و اگزالات در سرم ارزیابی گردید. واکاوی آماری از طریق آزمون تحلیل واریانس یک­طرفه و آزمون تعقیبی توکی انجام شد و 05/0p< معنی­دار در نظر گرفته شد.افزایش معنی­دار میزان اگزالات و کاهش معنی­دار کلسیم سرم در حیوانات گروه کنترل سنگ کلیوی در مقایسه با گروه کنترل سالم مشاهده شد. تیمار عصاره کرفس با غلظت 200 میلی­گرم بر کیلوگرم وزن بدن باعث کاهش معنی­دار اگزالات و افزایش معنی­دار کلسیم سرم در حیوانات دچار سنگ کلیوی شد.نتایج مطالعه حاضر نشان داد که عصاره کرفس می­تواند باعث بهبود سنگ ­کلیه ناشی از اتیلن ­گلیکول شود.
 

کلیدواژه‌ها

کرفس؛ سنگ‌کلیه؛ اتیلن‌ گلیکول؛ موش صحرایی

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