بررسی عیار سرمی آنتی‌بادی ضد مایکوباکتریوم‌ اویوم زیرگونه پاراتوبرکلوزیس به‌روش الایزا در گاوهای شیری هلشتاین

آسیب‌شناسی درمانگاهی دامپزشکی

مقاله 6، دوره 12، 2 (46) تابستان، شهریور 1397، صفحه 157-166   

نوع مقاله: مقاله علمی پژوهشی

نویسندگان

یاور حیدرزاده آسیابی¹؛ بهبود جعفری^{* 2}؛ علی حسن پور³

¹دانشجوی کارشناسی ارشد میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

²استادیار گروه میکروبیولوژی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران.

³دانشیار گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.

چکیده

بیماری یون از جمله عفونت­های مزمن باکتریایی در نشخوارکنندگان می‌باشد که با توجه به اسهال شدید و طولانی­مدتی که ایجاد می‌کند، باعث تحلیل رفتن بدن دام می­شود. هدف از این مطالعه، بررسی عیار سرمی آنتی­بادی ضد مایکوباکتریوم اویوم زیرگونهپاراتوبرکلوزیس (عامل بیماری یون) به روش الایزا و برآورد میزان آلودگی به عامل مذکور در گاوهای شیری مجتمع دامپروری کشت­و­صنعت مغان بود. در این مطالعه از تعداد 738 رأس گاو شیری بالغ در مجتمع کشت­و­صنعت و دامپروری مغان که به­طور تصادفی انتخاب شدند، خون­گیری شده و نمونه­های سرمی در آزمایشگاه به­روش الایزا بررسی شدند. مطالعه حاضر نشان داد از تعداد 738 رأس گاو مورد آزمایش، 58 رأس دارای عیار سرمی علیه مایکوباکتریوم اویوم زیرگونهپاراتوبرکلوزیس با درصد S/P بیشتر از 60 درصد بودند (میزان شیوع 86/7 درصد) که 25 رأس مثبت (با درصد S/P بیشتر از 70 درصد) و 33 رأس مشکوک (با درصد S/P بیشتر از 60 تا 70 درصد) بودند. به دلیل این که چهره بیماری یون اغلب تحت بالینی بوده و دام در ­ظاهر سالم به­نظر می­رسد، به سبب دفع میکروب در مدفوع و ترشحات بدن خود بقیه گله را نیز آلوده می­کند. بنابراین، انجام آزمون­های غربالگری دوره­ای در گله و همچنین تست دام­های تازه وارد در کنار سایر اقدامات پیشگیرانه اکیداً توصیه می­شود.

کلیدواژه‌ها

مایکوباکتریوم اویوم زیرگونه پاراتوبرکلوزیس؛ آنتی بادی؛ الایزا؛ گاوهای شیری

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