Spectroscopic studies on the interaction of Fe3O4@Ca Al LDH@Lamivudine with the calf thymus DNA

Journal of Nanoanalysis

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 11 مهر 1397

نوع مقاله: Original Research Paper

شناسه دیجیتال (DOI): 10.22034/jna.2018.565991.1086

نویسندگان

nahid shahabadi^{* 1}؛ mahtab razlansari¹؛ Avat (Arman) Taherpour²

¹razi university

²Organic Chemistry Department, Chemistry Faculty, Razi University, P.O.Box 67149-67346, Kermanshah, Iran

چکیده

In this study, we were synthesized and characterized of Fe3O4@LDH@Lamivudine by FT-IR spectroscopy, XRD and TEM. The interaction of this nanoparticle with CT-DNA was investigated by viscosity, circular dichroism (CD), Uv-visible and fluorescence spectroscopy. Among all nanocarriers which applied as drug delivery vectors Layered double hydroxides (LDHs) with exchangeable anions in the positive brucite-like interlayers have been attracting much attentions in the field of cellular delivery of anionic drug and other bio-functional molecules, due to their low toxicity, biocompatibility, high stability, pH dependent solubility and enhanced cellular uptake behavior compared with the conventional drug carriers. UV–Visible absorption studies indicated hyperchromism with the binding constant of 4.9×103 M-1. In the fluorimetric investigation, this nanocomposite can bind to DNA and creates a new non-fluorescence adduct. The thermodynamic parameters (ΔH

کلیدواژه‌ها

CT-DNA؛ Lamivudine؛ Layered Double Hydroxide؛ Fe3O4؛ Groove Binding