Acute Genotoxic Effects of Effluent Water of Thermo-Power Plant “Kosova” In Tradescantia Pallida

Journal of Chemical Health Risks

مقاله 5، دوره 1، شماره 1، آذر 2011   

شناسه دیجیتال (DOI): 10.22034/jchr.2011.543969

نویسندگان

I.R. Elezaj^* ؛ L.B. Millaku؛ R.H. Imeri-Millaku؛ Q.I. Selimi؛ K. Rr. Letaj

Department of Biology, University of Prishtina, P.O.Box 10.000 Prishtinë, Republic of Kosovo

چکیده

The aim of this study was the evaluation of acute genotoxic effect of effluent water of thermo-power plant by means of Tradescantia root tips micronucleus test (MN), mitotic index and cell aberrations.   Tradescantia, was experimentally treated (for 24 h), with effluent water of thermo-power plant in different dilution ratios (negative control – distilled water; primary untreated effluent water and 1:1; 1:2; 1:3; 1:4; 1:5; 1:6 and 1:7 respectively). Number of aberrant cells, and frequency of micronuclei (MN), in meristematic root tip cells of treated plants (Tradescantia), were significantly increased (P<0.001; P<0.001 respectively), while the mitotic index in all treated plants was progressively decreased in comparison to the negative control. The results of present study indicate that Tradescantia root-tip micronucleus assay with direct exposure of intact plants is an appropriate method which enables to detect genotoxic effects of effluent waters.

کلیدواژه‌ها

Tradescantia؛ MN-test؛ cell aberration؛ mitotic index

مراجع

1. De Rainho,C.R., A. Kaezner, C.A.F.Aiub, and I.
2. Felzenszwalb.2010. Ability of Alium cepa L.
3. root tips and Tradescantia pallida var.
4. puerpurea in N-nitrosodiethylamine
5. genotoxicity and mutagenicity evaluation. An
6. Acad Bras Cience. 82, (4), 921-932.
7. DeMarco A., S. Paglialunga, M. Rizzoni, A. Testa
8. and S. Trinca. 1988. Induction of micronuclei
9. in Vicia faba roots tips treated with heavy
10. metals (cadmium and chromium) in the
11. presence of NTA. Mutat. Res. 206, 311-315.
12. Eckel, P.M. 1995. Aquatic genotoxicity testing with
13. rat hepatocytes in primary culture II. Induction
14. of micronuclei and chromosomal aberrations.
15. Sci Total Environ. 159, 81-89.
16. Egito L. C. M., M.G. Medeiros, S. R. B. Medeiros,
17. and L.F. Agez-Lima. 2007. Cytotoxic and
18. genotoxic potential of surface water from the
19. Pitimbu river, northeastern /RN Brazil.
20. Genetics and Molecular Biology, 30 (2), 435-
22. Elezaj, I.R., K.H.Kurteshi, Q.I.Selimi, and
23. K.Rr.Letaj. 2003. Genotoxic effects of Sitnica
24. river (polluted from effluent waters of
25. ââ‚‌œKosovaââ‚‌ Power plant) in three fish species
26. (Barbus barbus, Perca fluviatilis and
27. Oncorhynchus muciss Walbaum). FASEB.
28. A1368/ 886.38.
29. Haider, T., R. Sommer, S. Knasmuller, P. Eckel,
30. W. Pribil, A. Cabaj and M. Kundi.2002.
31. Genotoxic response of Austrian groundwater
32. samples treated under standardized UV (254
33. nm)- disinfection conditions in a comb ination
34. of three different bioassays. Water Research.
35. , 25-32.
36. Helma C., S. Knasmuller , and R. Schulte Herman.
37. Bie Belastung von Wassern mit
38. genotoxischen Substanzen I. Methoden zur
39. Prufung der Genotoxizita. Z. Umweltchem
40. Okotox 6, 277-288.
41. Ivanova, E., A. S. Teodora, and I. Velcheva. 2005.
42. Cytogenetic testing of heavy metal and
43. cyanide contaminated river waters in a mining
44. region of Southwest Bulgaria. Journal of Cell
45. and Molecular Biology. 4, 00-106.
46. Knasmuller, S., E. Gottman, H. Steinkellner, A.
47. Fomin, Pickl C, A. Paschke, R. God, and M.
48. Kundi.1998. detection of genotoxic effects of
49. heavy metal contamination soils with plant
50. bioassays. Mutation Research., 2720; 000-000.
51. Knasmuller, S., C. Helma, P. M. Eckel, E.
52. Gottman, H. Steinkellner, F. Kassie, T. Haider,
53. W.Parzefall, and R. Schulte-Hermann. 1998.
54. Investigation on genotoxic effects of
55. groundwater from the Mitterndorfer Senke and
56. from the vicinity of Wiener Neustadt. 110, 23,
57. -833.
58. Kundi.1998. detection of genotoxic effects of heavy
59. metal contamination soils with plant bioassays.
60. Mutation Research., 2720;000-
61. micronucleus (TradMCN) bioassay on
62. clastogenicity of wastewater and in situ
63. monitoring. Mutat. Res. 270, 45-51.
64. Ma, T.H., 1982. Tradescantia cytogenetic tests
65. (root-tip mitosis, pollen mitosis, pollen mother
66. ââ‚‌“ cell meiosis). Mut. Res., 99, 293-302.
67. Ma, T.H., Z. Xu, C. Xu, H. McConnell, E. V.
68. Rabago, G.A. Arreola and H. Zang. 1995. The
69. improved Allium/Vicia root tip micronucleus
70. assay for clasto genicity of environmental
71. pollutants. Mutation Research - Environmental
72. Mutagenesis and related Subjects. 334 (2),
73. -195.
74. Majer, B., D. Tscherko, A. Paschke, R. Wennrich,
75. M. Kundi, E. Kandellr and S. Knasmuller.
76. Effects of heavy metal contamination of
77. soils on micronucleus induction in Tradescantia
78. and microbial enzyme activities: a
79. comparative investigation. Mutation Research.
80. , 111-124.
81. Matsumoto, S.T., M.S. Mantovani, M. Irene, A.
82. Malaguttii, A.L. Dias, I. C. Fonseca and M.A.
83. Marin-Morales. 2006. Genotoxicity and
84. mutagenicity of water contaminated with
85. tannery effluents, as evaluated by
86. micronucleus test and comet assay using fish
87. Oreochromis niloticus and chromosome
88. aberrations in onion root-tips. Genetics and
89. Molecular Biology. 29 (1), 148-158
90. Misk, M., T.H., Ma, A. Nersesyan, S. Monarca,
91. J.K. Kim and S. Knasmuller. 2011.
92. Micronucleus assays with the Tradescantia
93. pollen tetrads: and update. Mutagenesis., 26
94. (1), 215-221.
95. Odeigah, P.G.C., O. Nurudeen, and O.O. Amund .
96. Genotoxicity of oil field wastewater in
97. Nigeria. Hereditas. 126, 161-167.
98. Rizaj, M., Beqiri E, McBow, Oââ‚‌™Brein E.Z,
99. Kongoli F, 2008. The mineral base and
100. productive capacities of metals and nonmetals
101. of Kosovo. JOM., vol.60 No.8:18-22.
102. Ruiz, E. F., V. M. E. Rabago, S. U. Lecona, A. B.
103. Perez and T. H. Ma. 1992. Tradescantia ââ‚‌“
104. micronucleus (Trad-MCN) bioassay on clasto
105. genicity of wastewater and in situ monitoring.
106. Mutat. Res. 270, 45-51.
107. Samka-Kinci, V., P. Stegnar, M. Lovka and M. J.
108. Toman. 1996. The evaluation of waste,
109. Surface and ground water quality using the
110. Alium test procedure. Mutation Research/
111. Genetic Toxicology. 368 (3-4), 171-179.
112. Samuel , O.B., F.I. Osuala and P. G. C. Odeigah.
113. Cytogenotoxicity evaluation of two
114. industrial effluents using Allium cepa assay.
115. African Journal of Environmental Science and
116. Technology. 41 (1), 21-27.
117. ZSandhu, S.S., T.H. MA, Y. Peng and H. Zhou.
118. Clastogenicity of seven chemicals
119. commonly found at industrial waste sites.
120. Mutat. Res. 224, 437-445.
121. Shokal R.R., and F.J. Rholf. 1981. Biometry, 2nd
122. Edn., Freeman. New York, 1981, pp. 427-428.
123. Steinkenller, H., F. Kassie, and S.
124. Knasmuller.1999. Tradescantia ââ‚‌“ micronucleus
125. assay for the assessment of the clastogenicity
126. of Austrian water. Mutation Research. 426,
127. -116.
128. Tolbert, P. E., C. M. Shy, and J.W. Allen. 1992.
129. Micronuclei and other ano- malies in buccal
130. smears: methods Mutation Research, 271, 69-
132. Zahn, R. K., H. C. Schroder, and H. G.
133. Miltenburger. 1992