The Investigation of the Interaction between Human Serum Transferring with Colchicine in the Presence of Pb+2 Ions: Synchronous Fluorescence Measurements

Journal of Chemical Health Risks

مقاله 2، دوره 1، شماره 2، فروردین 2011   

شناسه دیجیتال (DOI): 10.22034/jchr.2012.543974

نویسنده

Goldouzian Z. Goldouzian F., Momen-Heravi M. and Chamani J.^*

چکیده

The interaction between Holo-Transferin (HTF) and Colchicine (COL) was investigated in the present of Pb+2 ions under physiological conditions by using synchronous fluorescence spectra. The synchronous fluorescence spectra show a slight change of tryptophan residue micro-environment. Synchronous fluorescence spectra show that the structure of the tyrosine residue environment was altered by interaction of the COL and Pb+2 ions with HTF. The fluorescence intensity of HTF decreased regularly beside a small blue shift with increasing concentrations of COL and Pb+2 ions. The intrinsic fluorescence of HTF was quenched in the presence of drug and ion. Interaction of drugs with HTF and HTF-Pb+2 can elucidate the properties of drug-protein and ion- protein complex, as it may provide useful information about the structural feature that determines the therapeutic effectiveness of ion and drugs. Therefore, it has become a significant research field in life science, chemistry, biotechnology and clinical medicine.

کلیدواژه‌ها

Holo-Transferinï€‌ CHTF؛ Colchicine (COL)؛ Nano- interactionï€‌ synchronous fluorescence spectra

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