DNA Biosensor for Determination of 5-Fluorouracil based on Gold Electrode Modified with Au and Polyaniline Nanoparticles and FFT Square Wave Voltammetry

Journal of Applied Chemical Research

مقاله 2، دوره 13، شماره 1، فروردین 2019، صفحه 24-35   

نوع مقاله: Research Paper

نویسندگان

Parviz Norouzi^{* 1}؛ Mohammad Amin Eshraghi²؛ Mehrnaz Ebrahimi¹

¹Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran

²Medical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran

چکیده

In the present study, a new biosensor for 5-Fluorouracil was described using modified gold
electrode and Fast Fourier transform square wave voltammetry (FFT SWV). Calf thymus DNA
immobilization was on a gold electrode decorated with polyaniline and gold nanoparticles. The
electrochemical characteristics of the electrodes were investigated by cyclic voltammetry, and
electrochemical impedance spectroscopy. After interaction DNA with the analyte, a dramatic
decline in [Fe(CN)6]/[Fe(CN)6]]3-/4- signal was observed which could be used as the signal. The
important parameters of the detection system, such as, pH were optimized. The linear dynamic
range of the biosensor was1×10-9 to 1×10-6 M (r2=0.996) limits of detection of
2.5×10−10 M. In addition to the wide linear range response, the proposed method demonstrated
acceptable sensitivity, repeatability and long-term stability. Future experiments could be performed
to examine several other effective factors so as to decrease the influence of the environment and
investigate its application to FFTSWV detection.

کلیدواژه‌ها

Electrochemical DNA biosensor؛ 5-Fluorouracil؛ Gold electrode modification؛ Fast Fourier transform voltammetry