Dynamic light scattering and zeta potential measurements: effective techniques to characterize therapeutic nanoparticles

Journal of Nanoanalysis

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 17 بهمن 1397

نوع مقاله: Review Paper

شناسه دیجیتال (DOI): 10.22034/jna.2019.575819.1108

نویسندگان

Abbas Rahdar^{* 1}؛ nooshin amini²؛ Fazeh Askari³؛ Md. Abu Bin Hasan Susan⁴

¹Department of physics, university of zabol, zabol, iran

²Master degree of Nanochemistry, Faculty of Pharmaceutical Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran (IAUPS)

³department of nanochemistry, university of Kharazmi

⁴Department of Chemistry, University of Dhaka, Dhaka 1000, Bangladesh

چکیده

Particle size and surface charge are key parameters of nanoparticles (NPs), especially therapeutic NPs, to influence the kinetics in vivo and interaction with the cellular and biological membranes and decide the efficacy for biological systems. Characterization of nanomaterials including both nanoparticles and micelles has therefore been a major issue in nanomedicine research to develop well-defined nano-formulations with focus on therapeutic goals. Dynamic light scattering (DLS) and zeta potential (ZP) measurements are widely accepted techniques for proper determination of the particle size and surface charge of therapeutic NPs. These have so far been easy, simple, and reproducible tools. However, there are challenges to interpret and systematically analyze data effectively due to lack of adequate understanding of the principles involved and impeccable background for operation of the system along with competence of sample preparation and characterization and so on. This review addresses the issues with focus on the fundamental principles involved in the techniques of DLS and ZP. The ultimate goal has been development of knowledge–base with a view to better analyzing and interpreting results for evaluation of hydrodynamic size, diffusion, inter particular interactions and stability of colloidal systems based on surface charge of therapeutic NPs.

کلیدواژه‌ها

Dynamic Light Scattering؛ Zeta Potential؛ Hydrodynamic Size