Parameters Affecting the Biosynthesis of Gold Nanoparticles Using the Aquatic Extract of Scrophularia striata and their Antibacterial Properties

Journal of Nanoanalysis

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 24 بهمن 1397

نوع مقاله: Original Research Paper

شناسه دیجیتال (DOI): 10.22034/jna.2019.579690.1120

نویسندگان

Yousef Naserzadeh^{* 1}؛ Niloufar Mahmoudi²؛ Elena pakina²؛ Imbia Anne Marie Wase²؛ Mohammad Heidari³؛ Alfred Khodaverdian⁴

¹agrobiotechnology department, RUDN university of Moscow,Russia

²Department of AgroBiotechnology, Institute of Agriculture, RUDN University, Moscow, Russia

³Department of Chemistry, Islamic Azad University, Arak Branch, Arak, Iran

⁴Department of Chemical Engineering, University of London, UK

چکیده

Green synthesis is a simple, low-cost, non-toxic, environmentally friendly and efficient approach to use. Leaf extract of plants rich in polyphenols, such as flavonoids, is a powerful agent in reducing the synthesis of gold nanoparticles. The purpose of this study is to investigate the parameters affecting the biosynthesis of gold nanoparticles using the aqueous extract of Scrophularia striata plant and their antimicrobial activity. Biosynthesis of gold nanoparticles was accomplished by the interaction of golden salt (HAuCl_4.3H_2 o) with aqueous extract of Scrophularia striata. In order to obtain uniform and spherical nanoparticles, the following parameters affecting the biosynthesis of nanoparticles were investigated and optimized by ultraviolet-spectrophotometric technique; golden salt concentration, extract volume, pH and reaction time. Transmission electron microscopy and X-ray diffraction technique were also used to further characterize nanoparticles. Finally, the anti-bacterial properties of gold nanoparticles were investigated by disc diffusion method. The resulting absorption spectra exhibited strong peaks at 570 nm, which is a specific wavelength for gold nanoparticles. Transmission electron microscopy studies showed that the gold nanoparticles had a spherical shape with a mean diameter of 5-10nm, and the highest diameter of the growth inhibition zone was observed on the diameter of the hafnium bacteria (14mm). In this study, it was observed that, with the aid of Scrophularia striata aqueous extracts, a golden nanoparticle showed an antibacterial activity against gram-negative bacteria.

کلیدواژه‌ها

biosynthesis؛ Scrophularia striata؛ Antimicrobial Activity؛ Gold nanoparticles