بررسی تنوع آلکالوئیدها در اندام‌های مختلف گیاهPhysalis divaricata D. Don. ، شهر کرد

اکوفیتوشیمی گیاهان دارویی

مقاله 8، دوره 6، شماره 1 - شماره پیاپی 21، خرداد 1397، صفحه 75-86   

نوع مقاله: مقاله پژوهشی

نویسندگان

الهام معلم¹؛ عبدالله قاسمی پیربلوطی^{* 2}؛ ایرج مهرگان³؛ طاهر نژادستاری³؛ علیرضا ایرانبخش³

¹گروه علوم گیاهی دانشگاه علوم تحقیقات تهران

²مرکز تحقیقات گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهر کرد

³گروه زیست شناسی گیاهی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد علوم تحقیقات تهران، تهران، ایران

چکیده

عروسک پشت پرده با نام علمی Physalis divaricata D. Don.، گیاهی علفی متعلق به تیره سیب زمینی یا تاج­ریزی (Solanaceae) دارای آلکالوئیدهای تروپانی: آتروپین، اسکوپولامین و فیزالین از سکواستروئیدها، می­باشد. در این پژوهش، از اندام­های مختلف (برگ، ساقه، ریشه، گل و میوه) گیاه عروسک پشت پرده در گل­خانه تحقیقاتی دانشگاه آزاد اسلامی واحد شهرکرد و زمان گلدهی در اردیبهشت 1394 برداشت گردید، عصاره اتانولی نمونه‌ها با استفاده از دستگاه سوکسلوله استخراج و به‌ترتیب میزان آتروپین، اسکوپولامین و فیزالین B با استفاده از کرماتوگرافی مایع با کارآیی بالاHPLC  ﺑﺎ ﻣﺤﺎﺳﺒﻪ ﺳﻄﺢ زﻳﺮ ﻣﻨﺤﻨﻲ­ﻫﺎی و ﻣﻘﺎﻳﺴﻪ آن­ها ﺑﺎ ﻣﻨﺤﻨﻲ­ﻫﺎی اﺳﺘﺎﻧﺪارد ﺑﺎ ﻣﻴﺎﻧﮕﻴﻦ ﺳﻪ ﺗﻜﺮار ﺑﺮای ﻫﺮﻧﻤﻮﻧﻪ اندازه‌گیری شد. نتایج ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦ در ﺳﻄﺢ  5 درﺻﺪ حاکی از آن است که بیشترین میزان فیزالین، اسکوپولامین و آتروپین، به‌ترتیب در عصاره­های میوه، گل و برگ‌های گیاه مشاهده گردید. بررسی یافته‌ها حاکی از تنوع فیتوشیمیایی مواد موثره در اندام­های مختلف گیاه بود.
 

کلیدواژه‌ها

آتروپین؛ اسکوپولامین؛ عروسک پشت پرده؛ فیزالینB؛ کروماتوگرافی مایع با کارآیی بالا

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