بررسی خصوصیات فیزیولوژیکی و فعالیت آنتی‌اکسیدانی ریحان (Ocimum basilicum cv. Keshkeni luvelou)تحت سطوح مختلف متیل‌جاسمونات و سمیت سرب

فیزیولوژی محیطی گیاهی

مقاله 1، دوره 13، شماره 51، آذر 1397، صفحه 1-16   

نوع مقاله: پژوهشی

نویسندگان

سمیه اسدی¹؛ محمد مقدم^{* 2}؛ عبدالله قاسمی پیربلوطی³؛ امیر فتوت⁴

¹گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

²گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهدف مشهد، ایران.

³مرکز تحقیقات گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهرقدس، تهران، ایران

⁴گروه علوم خاک، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

آزمایشی به منظور بررسی تاثیر متیل­جاسمونات بر خصوصیات فیزیولوژیکی و فعالیت آنتی­اکسیدانی گیاه ریحان تحت سمیت سرب بصورت فاکتوریل در قالب طرح کاملا ً تصادفی در سه تکرار در گلخانه تحقیقاتی دانشگاه فردوسی مشهد انجام شد. تیمارهای آزمایش شامل سه سطح نیترات سرب (صفر (شاهد)، 200 و 400 میلی­گرم در کیلوگرم خاک) و محلول­پاشی متیل­جاسمونات در سه غلظت (صفر، 5/0 و 1 میلی‌مولار) بودند. صفات مورد ارزیابی شامل میزان کلروفیل a و b، کلروفیل کل، کارتنوئیدها، نشت الکترولیت، رطوبت نسبی، فعالیت آنتی‌اکسیدانی، کربوهیدرات­های محلول، فنل کل و پرولین بود. نتایح حاصل از این مطالعه نشان داد که در گیاهان تیمار شده با سرب، میزان پرولین، محتوای نسبی آب، کلروفیلa ، فعالیت آنتی­اکسیدانی، فنل کل در مقایسه با گیاهان شاهد کاهش معنی­داری نشان داد و کاربرد متیل‌جاسمونات (5/0 میلی­مولار) در شرایط تنش سرب، باعث افزایش این صفات گردید. علاوه بر این در شرایط تنش سرب، میزان نشت الکترولیت به میزان قابل توجهی افزایش یافت و محلول­پاشی متیل­جاسمونات سبب کاهش آن شد. بنابراین، استفاده از متیل‌‌جاسمونات برای حفظ عملکرد اقتصادی گیاهان تحت تنش قابل توجیه است. همچنین استفاده از غلظت‌های 5/0 و 1 میلی­مولار آن در شرایط تنش عنصر سنگین سرب با تحریک ساخت آنتی اکسیدانت­ها، منجر به کاهش و تعدیل اثرات این تنش می­شود.

کلیدواژه‌ها

پرولین؛ تنظیم کننده رشد؛ رنگیزه‌های فتوسنتزی؛ محتوای نسبی آب؛ نشت الکترولیت

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