Antioxidant activity of methanol extract of Salvia aethiopis L. on circulatory system of rat

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 8، دوره 2، شماره 3، اسفند 2011، صفحه 217-221   

نوع مقاله: Original article

نویسندگان

Mostafa Asadbagi؛ Roya Karamian^* ؛ Parisa Hasanain؛ Masoud Ranjbar؛ Rametin Pakzad

Department of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran;

چکیده

Background & Aim: Free radicals play a crucial role in the development of tissue damage in various human diseases such as cancer, aging, neurodegenerative disease, malaria and arteriosclerosis, and pathological events in living organisms. Antioxidants may have an important role in the prevention of these diseases. The aim of this study is to evaluate potency of scavenging of DPPH free radicals in the circulatory system of rats by the methanolic extracts of Salvia aethiopis. This species belongs to the genus Salvia of the family Lamiaceae, many species of which are valuable in food and medicine. Further studies will indicate whether this plant can be as a suitable candidate for pharmacological and industrial applications. Experimental: Ten female rats (200-250 g) were randomly selected and then divided in three groups. The rats were injected intraperitoneally with 0.5 ml of 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical in methanol (3×10^-4 M), 1 ml of methanolic extract of S. aethiopis (1 mg/ml) along with 0.5 ml of methanolic solution of DPPH and 1 ml of ascorbic acid along with 0.5 ml of methanolic solution of DPPH as positive control, respectively. The absorbance of DPPH solution in the blood serum of rats was measured by spectrophotometric method in 517 nm. Results & discussion: Decrease in absorbance of DPPH free radical (1.85 nm) in circulatory system of the rats in presence of ascorbic acid as positive control (1.07 nm) and S. aethiopis extract (0.78 nm) as sample. It can be concluded that the potency of free radical scavenging by the metabolic extract of S. aethiopis in circulatory system of the rats is more than that of ascorbic acid. Industrial and practical recommendations: The Results from present investigation showed that S. aethiopis extract can be used as a natural compound with a high antioxidant activity in future studies.

کلیدواژه‌ها

Antioxidant activity؛ DPPH free radical؛ Rat؛ Salvia aethiopis

مراجع

قهرمان، ا.، 1373. کروموفیت­های ایران (سیستماتیک گیاهی). چاپ اول. مرکز نشر دانشگاهی، تهران.

مظفریان، و. 1382. فرهنگ نام­های گیاهان ایران: لاتینی، انگلیسی، فارسی.چاپ سوم. فرهنگ معاصر.

Ames, B. M., Shigena, M. K. and Hagen, T. M. 1993. Oxidants, antioxidants and the degenerative diseases of aging. Proceedings of the National Academy of Science. 90: 7915-7922.

Abiy, Y. Solomon, D. Jacob, O. M. Christine, C. B., Matthias, H. and Martin, G. P. 2005. Antimicrobial flavonoids from the stem bark of Erythrina burtii. Fitoterapia. 96: 496-499.

Bade, A. Z. M. A. and Farage, R. S. 1989. Antioxidant activity of some spice essential oils on linoleic acid oxidation in aqueous media. Journal of the American Oil Chemists' Society. 66: 345-356.

Bracco, U., Loliger, J. and Virret, J. L. 1981. Production and use of natural antioxidants. Journal of the American Oil Chemists' Society. 34: 686-690.

Cook, N. C. and Samman, S. 1996. Flavonoids: chemistry, metabolism, cardio protective effects and dietary sources. Nutritional Biochemistry. 7: 66-76.

Halliwell, B. 2000. The antioxidant paradox. Lancet. 355: 179-80.

Kelen, M. and Tepe, B. 2008. Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technology Journal. 99: 4096-104.

Kumpulainen, J. T. and Salonen, J. T. 1999. Natural antioxidants and anticarcinogens in nutrition, health and disease. The Royal Society of Chemistry. 3: 178-187.

Maxell, S. R. J. 1995. Prospects for the use of antioxidants therapies. Drugs. 49: 345-61.

Pham-Huy, L. H. and Pham-Huy, C. 2008. Free radicals, antioxidants in disease and health. International Journal of Biomedical Science. 4: 89-96.

Pokorny, J. Yanishlieva, N. and Gordon, M. 2001. Antioxidants in foods: practical applications, CRC Press.

Stadtman, E.R. 1992. Protein oxidation and aging. Science. 257: 1220-1224.

Yu, B. P. 1994. Cellular defenses against damage from reactive oxygen species. Physiolgical Reviews 74: 139-162.