The study of burn healing of Onosma stenosiphon on type II burn of back and testis areas in rats

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 4، دوره 1، شماره 1، شهریور 2010، صفحه 23-27   

نوع مقاله: Original article

نویسندگان

Mohammad Ali Khalili¹؛ Seyad Mohsen Miresmaeili^{* 2}؛ Hosein Hekmati Moghaddam³؛ Shahnaz Rezaei⁴؛ Ali Reza Vahidi⁵

¹Associate Professor, Department of Anatomy, Yazd University of Medical Sciences, Yazd, Iran

²ACECR Higher Education Institute, Yazd, Iran

³Research and Treatment for Infertility Center , Yazd University of Medical Sciences, Yazd, Iran

⁴Assistant Professor, Department of Pathology Yazd University of Medical Sciences, Yazd, Iran

⁵Assistant Professor, Department of Pharmacology, Yazd University of Medical Sciences, Yazd, Iran

چکیده

Background & Aim: Onosma stenosiphon has been used as herbal medicine for healing skin cuts and rashes. However, burn healing effect of this herb has not been yet reported in pharmaceutical texts. The aim of this study was to find out the effect of herb ointment on burn healing the superficial wounds post type II burns of back and testis region in Wistar rats.
Experimental: A total of 24 rats were divided into 3 (21 rats) and 1 control (3 rats) groups. Type II burn was performed on right and left sides of back and right testicular skin of all experimental animals. For burn healing, herb ointment was applied on right sides of back and testis skins of experimental rats. For histo-pathological evaluation, seven rats were killed at 7, 14, 21 days intervals. Then, skin samples were obtained from both left and right (with herb ointment) sides of back, as well as testicular tissue samples. All samples from experimental animals were compared with control samples following tissue staining.
Results & Discussion: All skin samples (both with and without herb ointment applications) showed patho-morphological changes one week post experimental burns. Two week following burns, epidermis were relatively healed, and lymphohistocytological inflammation with initiation of fibrosis was observed. But, epidermis and dermis inflammation were recovered after three weeks. There were no noticeable changes in healing rate of burned tissues in rats under herb ointment versus non ointment applications. Also, there were no alternations in testicular tissue or spermatozoa cells in experimental groups.

کلیدواژه‌ها

Onosma stenosiphon Boiss؛ Burn healing؛ Testis؛ Rats

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