Gastroprotective effects of Thymus daenensis hydroalcoholic extract against ethanol-induced gastric mucosal ulcers in rats

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 7، دوره 6، 3 Special issue (in Persian or Farsi)، بهمن 2015، صفحه 167-174   

نوع مقاله: Original article

نویسندگان

Firuzeh Saghaei^* ؛ Mohammad Salehi؛ Mohammad Javad Namzi؛ Mohammad Bagheri

Department of Pharmacology, Faculty of Veterinary Medicine , Islamic Azad University , Shahrekord branch, Shahrekord , Iran

چکیده

Background & Aim:  Ulcer index is one of the most common diseases among people. Current therapies include acid secretion inhibitors which have various side effects. Herbal medicines are considered as adjuvant therapy for this disease. Hydroalcoholic extract of Thymus daenensis has various components such as flavonoids which contribute to gastroprotection. Experimental: 35 Sprague Dawley rats (200-250 g weight) were randomly divided into 7 groups: Group 1 received salin, Groups 2 and 3 and 4 (experimental groups) received ethanolic extract of Thymus daenensis (500 and 1000 and 1500 mg/kg respectively), Group 5 received omeprazole 20 mg/kg., Group 6 received orally carboxy methyl cellulose (omeprazol vehicle), Group 7(negative control) received saline. One hour later absolute ethanol was given orally to all rats except group 7 to induce gastric mucosal injury. After 4 hrs. the rats were anesthetized and sacrificed. Blood samples were collected through heart and were administrated to SOD measurement and gastric tissues were removed for histopathologic study and MDA assessment. Results & Discussion: The extract of plant was a significant and dose dependent effect to reduce ulcer index and to increase the percentage of ulcer inhibition. The study also indicated a significant increase in SOD activity in serum, whereas a significant decrease in MDA was observed. The ulcer protective effect of this plant may possibly be due to antioxidant effects and reduction of oxidative stress.

کلیدواژه‌ها

Thymus daenensis؛ Rat- gastric ulcer؛ Super oxide dismutase؛ Malon dialdehyde

مراجع

Abul, H., Mathew, T. C., Dashti, H. M. and Al- Bader, A. 2002. Level of superoxide dismutase, glutathione peroxidatione and uric acid in thioacetamide-induced cirrhotic rats. Anatomia Histoogial Embryologia., 31(2): 66-71.

Alvarez- Suarez,  J. M., Dekanski, D., Ristic, S., Radonjic, N. V., Petronijevic, N. D., Giampieri, F., Astolfi, P., Gonzales, A. M., Santos, B. G., Tulipani, S., Quiles, J. L., Mezzetti, B. and Battino, M. 2011. Strawberry Polyphenols Attenuate Ethanol-Induced Gastric Lesions in Rats by Activation of Antioxidant Enzymes and Attenuation of MDA Increase. PLOS ONE., 6(10): e 25878.

Amiri, H. 2011. Essential oils composition and antioxidant properties of three thymus species. Evidence-Based Complementary and Alternative Medicine., Volume 2012, 8 pages.

Bastid, C., Tellechea, J. and Sahle, J. 1990. Does jaundice increase the frequency of gastroduodenal ulcerations?  Hepatogastroenterology., 37(6): 612-614.

Bradi, D. A. A., Sarah Khan, M. A., Sabri, S. Z., Kadir, F. A., Mahmood, A. A., Zahra, A. A., Suzy, M., Al-Hanhana, N. and Al-Magrami, A. 2011. Anti ulcerogenic activity of typhonium flagelliforme aqueous leaf extract against ethanol-induced gastric mucosal injury in rats. Scientific research and Essay., 6(15): 3232-3239.

Biswas, K., Banyopadhyay, U., Chattopadyay, I., Varadaraj, A., Ali, E. and Banerjee, R.K. 2003. A novel antioxidant and antiapoptotic role of omeprazole to block gastric ulcer through scavenging of hydroxyl radical. Biological Chemistry., 278(13): 10993-11001.

Casper, f., Houser, H., Longo, J., Jameson, H., Lvskal. 2015. Harrison Principles of Internal Medicine: diseases of the digestive system. Mighty. nineteenth edition. 264

Cuevas, M., Calzado, Y. R., Guerra, Y. P., Year, A. O., Despaigne, S. J., Ferreiro, R. M. and Quintana, D. C. 2011. Effects of Grape Seed Extract, Vitamin C, and Vitamin E on Ethanol and Aspirin Induced Ulcers. Advances in Pharmacological Sciences., Volume 2011 (2011), Article ID 740687, 6 pages.

Esterbauer, H. and Cheeseman, KH. 1990. Determination of aldehydic lipid peroxidation products:  malonaldehyde and   hydroxynonenal. Methods in Enzymology., 186: 407-21

Ibrahim, IA., Qader, S.W., Abdolla, M.A., Nimir, A.R., Abdelwahab, S.I. and Al-bayati, F.H. 2012. Effects of Pithecellobium Jiringa Ethanol Extract against Ethanol-Induced Gastric Mucosal Injuries in Sprague-Dawley Rats. Molecules., 17(3): 2796-811.

Iinuma, S., Yoshikawa, T., Yoshida, N., Naito, Y. and Kondo, M. 1998. Role of active oxygen species and lipid peroxidation in Mepirizole- induced duodenal ulcers in rats. Digestive Diseases and Sciences., 43(8): 1657-64.

Jaiswal, S. K., Rao, C. V., Sharman, B., Mishra, P., Das, S. and Dubey, M.K.  2011. Gastroprotective effect of standardized leaf extract from Argyreiaspeciosa on experimental gastric ulcers in rats. Ethnopharmacology., 137(1): 341–4.

Jankowski, J., Bridges, A. B., Scott, N., Wormsley, K. G. and Belch, J. J. F. 1991. Circulating free-radical markers and peptic-ulcer disease. European Journal of Gastroenterology & Hepatology., 3(11): 823-828.

Karatas, F., Karatepe, M. and Baysar, A. 2002. Determination of free malondialdehyde in human serum by high-performance liquid chromatography. Analytical Biochemistry., 311(1): 76-79.

Kobayashi, T., Ohta, Y., Inui, K., Yoshino, J. and Nakazawa, S. 2002. Protective effects of omeprazole against acute gastric mucosal lesions induced by compound 48/80, a mast cell degranulator, in rats. Pharmacological Research., 46(1): 75-84.

Konturek, S.J., Brozozowski, T. and Radecki, T. 1983. Protective action of omeprazole a benzimidazole derivative, on gastric mucosal damage by aspirin and ethanol in rats. Digestion., 27(3): 159-64.

Lapenna, D., de Gioia, S., Ciofani, G., Festi, D. and Cuccurullo, F. 1996. Antioxidant properties of omeprazole. FEBS Letters., 382(1-2): 189-92.

Mattsson, H., Andersson, K. and Larsson, H. 1983. Omeprazole provides protection against experimentally induced gastric mucosal lesions. European Journal of Pharmacology., 91(1): 111-4.

 Nazari, M., Monajemi, R., Ghasemipirbalouti, A.,   Jafarian Dehkordi, M., Riahi Dehkordi, M. 2013. Effects of essential oils of Thymus deanensis and Satureja bachtiarica on plasma lipoproteins in rats feeding with a fatty diet. Juornal of Herbal Drugs., 3(4): 243-248 

Ravindranath, B. 1989. Principles and practice of chromatography. Ellis Horwood series in analytical chemistry., pp: 34-50.

 Rang, H., P. Ritter, J. M., Flower, R. J. and Henderson, G. 2003. Rang and Dale s Pharmacology. Churchill-Livingstone. Fifth edition, PP: 485-6, 493-4 and 726-8.

Saghaei, F., Karimi, I., Jouyban, A. and Samini, M. 2012. Effects of captopril on the cysteamine-induced duodenal ulcer in the rat. Experimental and Toxicologic Pathology., 64(4): 373-377.

Santra, A., Chowdhury, A., Chaudhuri, S., Das Gupta, J., Banerjee, P. K. and Mazumder, D. N. 2000. Oxidative stress in gastric mucosa in helicobacter pylori infection. Indian Journal of Gastroentrology., 19(1): 21-3.

Shirpoor, A., Ansari, M. H., Salami, S., Pakdel, F. G. and Rasmi, Y. 2007. Effect of vitamin E on oxidative stress status in small intestine of diabetic rat. World Journal of Gastroenterology., 13(32): 4340-4.

Urbanana vicius, A. 1997. Free radical damages in proteins. Biochemistry., 324: 1-18.      

Wandall, J. H. 1992. Effects of omeprazole on neutrophil chemotaxis, superoxide production, degradation and translocation of cytochrome b-245.Gut., 33: 617-21.