Providing a mathematical model for measuring the expression of GUS gene was transferred temporarily through xylem vessels using RT-PCR and probe Gold nanoparticles

International Journal of Bio-Inorganic Hybrid Nanomaterials

مقاله 1، دوره 5، شماره 3، مهر 2016، صفحه 159-165   

نوع مقاله: Research Paper

چکیده

Gene transfer to plants and the production of transgenic plants with various purposes, such as improving the performance and quality, resistance to pests, diseases, etc., and of great importance are carried out Gene transfer to plants performs to evaluate the transient and permanent gene expression. Transient expression is quick, easy and simple and is not influenced by position effect compare to the permanent expression. Due to the advantages of Agrobacterium-mediated gene transfer methods, including simple, inexpensive, and accurate and being natural, in this study, development of a new method of Agrobacterium-mediated gene transfer to plants through the wooden vessel Transient Expression of GUS gene using RT-PCR method and the gold nanoparticle probes. However, the use of detectors attached to gold nanoparticles to detect biochemical and molecular techniques in the specificity and high speed and lower costs can be performed. The intron GUS reporter gene plasmid pCAMBIA1305.2 of Agrobacterium-mediated plant transformation was tested and the results of gene expression between them were analyzed using a mathematical model. The results of this study showed that gene transfer through the stem and the stem xylem vessels associated with two factors, so that the square of the radius of the stem directly and inversely proportional to its length.

کلیدواژه‌ها

Gold nanoparticles؛ GUS gene؛ Mathematical model؛ RT-PCR؛ pCAMBIA1305.2 plasmid