A novel optical DNA biosensor for detection of trace concentration of Methylene blue using Gold nano-particles and Guanine rich single strand DNA

International Journal of Nano Dimension

مقاله 5، دوره 8، شماره 3، مهر 2017، صفحه 224-233   

نوع مقاله: Reasearch Paper

نویسندگان

Rasoul Pourtaghavi Talemi^* ؛ Mohammad Hossein Mashhadizade

Faculty of Chemistry, Kharazmi University, Tehran, Iran

چکیده

The glass surface modification with 3-(mercaptopropyl) trimethoxysilane (MPTS), gold nano-particles (GN) and guanine rich single strand DNA (ss-DNA) was utilized as a novel and efficient platform for sensing trace concentration of methylene blue (MB) by an inexpensive spectrophotometric method. Methylene Blue (MB) can interact with the guanine base of single strand DNA and absorbed onto glass surface and its absorbance can be determined at λmax of MB. Increase in methylene blue absorbance was linear with an increase in MB concentration and can be viewed by the naked eye and detected by spectrophotometric method. The linear range of the developed biosensor for determination of MB was from 10 to 100 nmol L-1 with a detection limit of 4 nmol L-1. The fabricated spectrophotometric MB biosensor possessed excellent selectivity and good sensitivity.

کلیدواژه‌ها

Glass Surface؛ Gold Nano-particles؛ Guanine؛ Methylene blue؛ Optical DNA Biosensor؛ Single Strand DNA

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