Potential benefits of foliar application of chitosan and Zinc in tomato

Iranian Journal of Plant Physiology

مقاله 3، دوره 9، شماره 2، فروردین 2019، صفحه 2703-2708   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2019.664574

نویسندگان

Azam Salimi¹؛ Zahra Oraghi Ardebili^{* 2}؛ Maryam Salehibakhsh¹

¹Department of Biology, Kharazmi University, Karaj, Iran

²Department of Biology,Garmsar Branch, Islamic Azad University, Garmsar, Iran

چکیده

The current study was carried out to investigate the efficiencies of foliar supplementations of Zinc and/or chitosan on the growth and physiology of tomato (Lycopersicon esculentum L.) and clarify the involved mechanisms. Seedlings were sprayed with three concentrations (0, 50, and 100 mgL^-1) of chitosan and/or three levels of Zinc sulfate (0, 50 and 100 mgL^-1). The application of Zn and/or chitosan led to increases in shoot fresh mass, about 31%, over control. In comparison with the control, enhancement (approximately 28.5%) in shoot dry mass resulted from the single application of chitosan or zinc while this percentage reached to about 45% for seedlings simultaneously treated with the chitosan and Zn supplements. About 29% improvements in the plant height resulted from the chitosan and/or zinc. Higher amounts of chlorophyll contents were recorded in the chitosan and/or zinc-treated plants, among which the highest levels were found in the combined treatments. Simultaneous applications of chitosan and zinc were the most effective treatments to induce PAL (phenylalanine ammonia lyase) activity (about 64%) when compared to the control. Chitosan and/or zinc treatments, especially the latter, significantly promoted the activity of (SOD) superoxide dismutase enzyme (about two folds), over the control. Also, increases in proline contents was provoked by applying the treatments. Foliar supplementations of these compounds as an eco-friendly solution may have considerable potential to act as exogenous elicitors and trigger various physiological traits, thereby improving plant growth and resistance under abiotic stress conditions.
 

کلیدواژه‌ها

Bio-fortification؛ Chitosan؛ elicitor؛ foliar؛ Lycpersicon

مراجع

Ahmed, A., H., M. Khalil, A Abd El-Rahman and A. Nadia. 2012.  'Effect of zinc, tryptophan and indole acetic acid on growth, yield and chemical composition of Valencia orange trees'. Journal of applied sciences research, 8: 901-914.

Anusuya , S. and M. Sathiyabama. 2016. 'Effect of chitosan on growth, yield and curcumin content in turmeric under field condition'. Biocatalysis and Agricultural Biotechnology, 30:102-106.

Ardebili, Z.O., N.O. Ardebili, S. Jalili and S. Safiallah. 2015. 'The modified qualities of basil plants by selenium and/or ascorbic acid'. Turkish Journal of Botany, 39: 401-407.

Arnon, D.I. 1949. 'Copper enzymes in isolated chloroplasts'. Polyphenol oxidase in Beta vulgaris. Plant Physiology, 24(1): 1–15.

Asgari-Targhi, G., A. Iranbakhsh and Z.O. Ardebili. 2018. 'Potential benefits and phytotoxicity of bulk and nano-chitosan on the growth, morphogenesis, physiology, and micro propagation of Capsicum annuum'. Plant Physiology and Biochemistry, 127: 393-402.

Bates, L.S., R. Waldren, and I. Teare. 1973. 'Rapid determination of free proline for water-stress studies'. Plant and soil, 39: 205-207.

Beaudoin-Eagan, L.D. and T.A. Thorpe. 1985. 'Tyrosine and phenylalanine ammonia lyase activities during shoot initiation in tobacco callus cultures'. Plant Physiology, 78: 438-441.

Bradford, M.M. 1976. 'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding'. Analytical Biochemistry, 72: 248-254.

Cakmak, I. 2008. 'Enrichment of cereal grains with zinc: agronomic or genetic biofortification?'. Plant Soil, 302: 1-17.

El Hadrami, A., L. Adam, I. El Hadrami and F. Daayf. 2010. 'Chitosan in plant protection'. Marine drugs, 8: 968-87.

Giannopolitis, C.N. and S. Ries. 1977. 'Superoxide dismutase I. Occurrence in higher plants'. Plant Physiology, 59: 309-314.

Hotz, C. and K. Brown. 2004. 'Assessment of the risk of zinc deficiency in populations and options for its control'. International nutrition foundation: for UNU.

Iriti, M., V. Picchi, M. Rossoni, S. Gomarasca, N. Ludwig, M. Gargano and F. Faoro, 2009. 'Chitosan antitranspirant activity is due to abscisic acid-dependent stomatal closure󠇦'. Environmental and Experimental Botany, 66: 493-500.

Khan, W.M., B. Prithiviraj and D. Smith. 2002. 'Effect of foliar application of chitin and chitosan oligosaccharides on photosynthesis of maize and soybean'. Photosynthetica,40(4): 621-624.

Li, K., X. Lu and L. Peng, 2001. 'Effects of carboxymethyl chitosan on key enzymes activities of nitrogen metabolism and grain protein contents in rice'. Journal-Hunan Agricultural University, 27(6): 421-424.

Mondal, M.M., M. Malek, A. Puteh, M. Ismail, M. Ashrafuzzaman and L. Naher.2012. 'Effect of foliar application of chitosan on growth and yield in okra'. Australian Journal of Crop Science, 6: 918-921.

Nge, K.L., N. Nwe, S. Chandrkrachang and W. Stevens. 2006. 'Chitosan as a growth stimulator in orchid tissue culture'. Plant Science, 170:1185-1190.

Pichyangkura, R. and S. Chadchawan. 2015. 'Biostimulant activity of chitosan in horticulture'. Scientia Horticulture, 196:49-65.

Pirbalouti, A.G., F. Malekpoor, A. Salimi and A. Golparvar. 2017. 'Exogenous application of chitosan on biochemical and physiological characteristics, phenolic content and antioxidant activity of two species of basil (Ocimum ciliatum and Ocimum basilicum) under reduced irrigation'. Scientia Horticulture, 217: 114-22.

Simoglou, K.B. and C. Dordas. 2006. 'Effect of foliar applied boron, manganese and zinc on tan spot in winter durum wheat'. Crop Protection, 25: 657-663.

Subramanian, K.S., C. Bharathi, N. Gomathy and N. Balakrishnan. 2014. 'Role of arbuscular mycorrhizal ('Glomus intraradices') fungus inoculation on Zn nutrition in grains of field grown maize'. Australian Journal of Crop Science, 8(5):655-665.

Suvannasara, S.B., P. Promsomboon and K. Boonlertnirun. 2011. 'Application of chitosan for reducing chemical fertilizer uses in waxy corn growing'. Thai Journal of Agricultural Science, 44(5): 22-28.

Taiz, L. and E. Zeiger. 2010. Plant physiology 5th Ed. Sunderland, MA: Sinauer Associates.

Vasil’ev, L.A., E. Dzyubinskaya, R. Zinovkin, D. Kiselevsky, N. Lobysheva and V. Samuilov. 2009. 'Chitosan-induced programmed cell death in plants'. Biochemistry, 74:1035-1043.

Yavas, I. and A. Unay. 2016. 'Effects of zinc and salicylic acid on wheat under drought stress'. Journal of Animal and Plant Sciences, 26: 1012-1018.

Zhang, Y.Q., Y. Sun, Y. Ye, M. Karim, Y. Xue, P. Yan, Q. Meng, Z. Cui, I. Cakmak, F. Zhang and C. Zou. 2012.' Zinc biofortification of wheat through fertilizer applications in different locations of China'. Field Crops Researches, 125: 1-7.

Zuppini, A., B. Baldan, R. Millioni, F. Favaron, L. Navazio and P. Mariani. 2004. 'Chitosan induces Ca²⁺-mediated programmed cell death in soybean cells'. New phytologist, 161: 557-568.