Influence of plant growth regulators (BA, TDZ, 2-iP and NAA) on micropropagation of Aglaonema widuri

Iranian Journal of Plant Physiology

مقاله 4، دوره 9، شماره 2، فروردین 2019، صفحه 2709-2718   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2019.664575

نویسندگان

Behazd Kaviani^{* 1}؛ Shahram Sedaghathoor¹؛ Mohammad Reza Safari Motlagh²؛ Seddigeh Rouhi¹

¹Department of Horticultural Science, Rasht Branch, Islamic Azad University, Rasht, Iran

²Department of Plant Pathology, Rasht Branch, Islamic Azad University, Rasht, Iran

چکیده

Aglaonema widuri is an evergreen and indoor ornamental plant. This study aimed to investigate the effect of some plant growth regulators on micropropagation of A. widuri. A protocol was developed for high frequency in vitro multiplication of A. widuri on the same medium for both shoots and roots induction. This condition decreases time duration and cost of micropropagation. Apical buds as explants were obtained from greenhouse-grown A. widuri and were established on Murashige and Skoog (MS) medium. Three cytokinins [N⁶-benzyladenine (BA; 0.00, 3.00, 3.50, and 4.00 mgL-1), 1-phenyl-3-(1,2,3-thiaiazol-5-yl) urea (TDZ; 0.00, 0.50, and 1.00 mgL-1), and N⁶-(2-isopentenyl) adenine (2-iP; 0.00 and 7.00 mgL-1)] and one auxin [α-naphtaleneacetic acid (NAA; 0.00, 0.10, 0.20, 0.30, and 0.40 mgL-1)] were studied for their effect on miropropagation of A. widuri. BA and NAA treatments as 3.00 mgL-1 + 0.2 mgL-1 recorded the highest shoot proliferation rate (number: 6.00 shoots and length: 7.75 cm per explant). Treatment of 4.00 mgL-1 BA + 0.10 mgL-1 NAA + 0.50 mgL-1 TDZ produced maximum nodes (13.25 per explant). The largest number of leaf (4.25 per explant) was produced in the medium containing 3.50 mgL-1 BA + 0.20 mgL-1 NAA. Maximum root initiation and development (14.25 per explant) was obtained on medium containing 3.00 mgL-1 BA + 0.20 mgL-1 NAA. The combination of 3.50 mgL-1 BA + 0.20 mgL-1 NAA was found to be the most suitable growth regulators for obtaining the highest root length (8.25 cm per explant). The plantlets were transferred to pots and grown in the greenhouse with a success rate of 95%.

کلیدواژه‌ها

Araceae؛ in vitro micropropagation؛ ornamental plants؛ phytohormones؛ tissue culture

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