Biological activities of the methanolic extracts and compounds from leaves and twigs of Diospyros zenkeri (Gürke) F. White (Ebenaceae)

Trends in Phytochemical Research

مقاله 5، دوره 3، شماره 2، شهریور 2019، صفحه 117-122   

نوع مقاله: Original Article

نویسندگان

Hermann Feumo Feusso^{1، 2}؛ Jean de dieu Dongmo¹؛ Carine Mvot Akak³؛ Mehreen Lateef⁴؛ Ayaz Ahmed⁵؛ Anatole Guy Blaise Azebaze¹؛ Alain François Kamdem Waffo¹؛ Muhammad Shaiq Ali²؛ Juliette Catherine Vardamides^{* 1}

¹Department of Chemistry, Faculty of Science, University of Douala P.O. Box 24157 Douala-Cameroon

²H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Karachi-75270, Pakistan

³Department of Organic Chemistry, Faculty of Science, University of Yaounde I P.O. Box 812 Yaounde-Cameroon

⁴Multi-Disciplinary Research Laboratory Bahria University Medical and Dental College, Karachi, Pakistan

⁵Dr. Panjwani Center for Molecular Medicine and Drug Research International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

چکیده

This study aimed for investigating chemical constituents and biological activities of Diospyros zenkeri (Gürke) F. White. 14 known secondary metabolites have been isolated from the leaves and the twigs of D. zenkeri such as 3-methoxy-7-methyljuglone (1), β-carotene (2), lupeol (3), mixture of β-sitosterol (4) and stigmasterol (5), betulin (6), ursolic acid (7), messagenin (8), 3β,28,30-lup-20(29)-ene triol (9), mixture of the glucosides of stigmasterol (10) and β-sitosterol (11), norbergenin (12), betulinic acid (13) and vanillic acid (14), respectively . The structures of the compounds were elucidated with the help of NMR and mass spectral studies. The compounds 8, 9 and 14 are reported for the first time from the genus Diospyros. The biological screening of all the isolates and the crude methanolic extracts have been carried out including antiproliferative activity, antioxidant potential and inhibitory activity against the enzymes lipoxygenase and urease, respectively. Compound 1 exhibited significant antiproliferative activity against two cancer cell lines CAL-27 (IC50=2.98 μM) and NCI-H460 (IC50=5.57 μM). Methanolic extracts of the leaves and twigs of D. zenkeri presented low antiproliferative activity against these two cancer cell lines. Compounds 1, 6, 8, 9, 12 and the crude extracts exhibited moderate antioxidant activity with IC50 values of 76.5 μM, 65.8 μM, 55.3 μM and 51.2 μM respectively compared to BHA (IC50=44.2 μM). Compounds 6, 8 and 9 showed moderate lipoxygenase inhibition activity with IC50 values of 58.5 μM, 52.8 μM and 58.8 μM, respectively compared to baicalein (IC50=22.6 μM).

کلیدواژه‌ها

Biological activities؛ Diospyros zenkeri (Gürke) F.White؛ Ebenaceae؛ Leaves؛ Secondary metabolites؛ Twigs

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