An insight to optical studies of acridine orange cationic dye within nanometer-sized microemulsions at fixed water content

Journal of Nanoanalysis

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 04 دی 1398

نوع مقاله: Original Research Paper

شناسه دیجیتال (DOI): 10.22034/jna.2019.1881565.1165

نویسندگان

Mousa Aliahmad¹؛ Havva ESMAILZAEE¹؛ Abbas Rahdar^{* 2}؛ Bijan Paul³

¹Department of Physics, University of Sistan and Baluchestan, Zahedan, P. O. Box. 35856-98613, Islamic Republic of ‎Iran

²Department of physics, university of zabol, zabol, iran

³Department of Chemistry, Jadavpur University, Kolkata 700032, India

چکیده

Cationic dye Acridine orange (AO) has wide applications especially in biological fields such as analysis of lysosomal and mitochondria content by flow cytometry and so on. In the current work, spectroscopy of acridine orange (AO) dye at both low concentrations (mdye/mwater=6.25*10-5, 3.12*10-5) and high concentrations (mdye/mwater=0.002, 0.001) was studied in confined water nanodroplets within water/AOT/n-hexane microemulsions (MEs) at a constant water content (W= [Water]/[AOT]=10) and as a function of mass fraction of droplet (MFD) using absorption and fluorescence spectroscopic techniques. The absorption spectra of the dye at high concentrations of Acridine orange (AO) dye molecules showed that the absorption spectra of the samples deviated from Beer's law, and are broadened at larger MFD due to the interactions of AO dye molecules. The fluorescence spectrum was investigated at two high concentrations (0.002, 0.001) and low concentrations (6.25*10-5, 3.12*10-5). At high concentration of the dye, quenching of fluorescence intensity was observed due to the accumulation of the dye molecules, coupled with a red shift with increasing MFD. However, in the lower concentration regime, enhancement of fluorescence intensity was observed with increasing MFD. The Stokes’ shift of the dye for both high and low concentrations increased with MFD, but to a greater extent at high concentrations compared to that at low concentrations.

کلیدواژه‌ها

Dye؛ Acridine orange ، microemulsions، spectroscopy