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Hekmat, Azadeh, Afrough, Masoumeh, Hesami Tackallou, Saeed, Ahmad, Faizan. (1398). Synergistic effects of Titanium dioxide nanoparticles and Paclitaxel combination on the DNA structure and their antiproliferative role on MDA-MB-231cells. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, (), -. doi: 10.22034/jna.2020.1869287.1141
Azadeh Hekmat; Masoumeh Afrough; Saeed Hesami Tackallou; Faizan Ahmad. "Synergistic effects of Titanium dioxide nanoparticles and Paclitaxel combination on the DNA structure and their antiproliferative role on MDA-MB-231cells". نشریه علمی پژوهشی دانشگاه آزاد اسلامی, , , 1398, -. doi: 10.22034/jna.2020.1869287.1141
Hekmat, Azadeh, Afrough, Masoumeh, Hesami Tackallou, Saeed, Ahmad, Faizan. (1398). 'Synergistic effects of Titanium dioxide nanoparticles and Paclitaxel combination on the DNA structure and their antiproliferative role on MDA-MB-231cells', نشریه علمی پژوهشی دانشگاه آزاد اسلامی, (), pp. -. doi: 10.22034/jna.2020.1869287.1141
Hekmat, Azadeh, Afrough, Masoumeh, Hesami Tackallou, Saeed, Ahmad, Faizan. Synergistic effects of Titanium dioxide nanoparticles and Paclitaxel combination on the DNA structure and their antiproliferative role on MDA-MB-231cells. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 1398; (): -. doi: 10.22034/jna.2020.1869287.1141

Synergistic effects of Titanium dioxide nanoparticles and Paclitaxel combination on the DNA structure and their antiproliferative role on MDA-MB-231cells

Journal of Nanoanalysis
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 27 بهمن 1398
نوع مقاله: Original Research Paper
شناسه دیجیتال (DOI): 10.22034/jna.2020.1869287.1141
نویسندگان
Azadeh Hekmat* 1؛ Masoumeh Afrough1؛ Saeed Hesami Tackallou2؛ Faizan Ahmad3
1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
3Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India
چکیده
Purpose: The objective of this investigation was to evaluate the synergistic effect of paclitaxel (PTX) combined with titanium dioxide nanoparticles (TiO2NPs) on DNA structure and to examine the proliferation of MDA-MB-231cells.
Methods: This investigation performed with Ultraviolet spectroscopy, zeta potential investigation, circular dichroism (CD) spectroscopy, ELISA reader and fluorescence spectroscopy.
Results: The Ultraviolet results indicated that the structure of DNA in the presence of PTX‎ and TiO2NPs (at a lower concentration) ‎changed significantly rather than TiO2NPs or PTX alone. The fluorescence results exposed that PTX+TiO2NPs could form a complex via non-intercalative mechanism and the PTX+TiO2NPs affinity to DNA increased considerably. The thermodynamics parameters displayed that PTX+TiO2NPs interact with DNA strongly and in this interaction, the hydrophobic force plays an important role. The CD data confirmed that DNA structure was modified by PTX+TiO2NPs via a simple and reasonable mechanism: change in DNA conformation from B to C-form. The negative charge of DNA reduced strongly after addition of PTX+TiO2NPs. The anticancer property of PTX+TiO2NPs by MTT assay demonstrates that this combination can tremendously diminish the proliferation of MDA-MB-231cells compared to PTX or TiO2NPs alone.
Conclusion: Based on this investigation TiO2NPs could enhance the affinity and binding of PTX (at a lower concentration) on DNA structure and PTX+NDs can promote mortality of MDA-MB-231 cells. This study can ‎offer an innovative strategy for designing‎ the ideal anti-tumor agents.
کلیدواژه‌ها
Titanium dioxide nanoparticles (TiO2NPs)؛ Paclitaxel (PTX)؛ Spectroscopy؛ C-form DNA؛ MDA-MB-231cells
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تعداد مشاهده مقاله: 517


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