بررسی اثر نانوذره اکسیدروی بر رشد، رنگیزه‌های فتوسنتزی و اسمولیت‌های محلول گیاه نوروزک (Salvia leiifolia Benth.) تحت شرایط تنش شوری

فیزیولوژی محیطی گیاهی

مقاله 4، دوره 14، شماره 56، دی 1398، صفحه 74-93   

نوع مقاله: پژوهشی

نویسندگان

مهدی آخوندی¹؛ مریم نیاکان^{* 1}؛ هما محمودزاده²؛ مجید دشتی³

¹گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد گرگان، گرگان، ایران

²گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد مشهد، مشهد، ایران

³مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران

چکیده

تنش شوری یکی از اصلی‌ترین عوامل محدودکننده رشد و تولید گیاهان می‌باشد. عنصر روی ازجمله عناصر ضروری در رشد و فرایندهای متابولیسمی می‌باشد که مصرف آن سبب مقاومت گیاهان به تنش‌های محیطی می‌شود. از سوی دیگر کاربرد عناصر در شکل نانو به‌علت سهولت جذب عناصر امروزه مورد توجه قرارگرفته است. به‌منظور بررسی اثر محلول‌پاشی نانواکسیدروی در شرایط تنش شوری، آزمایشی در قالب طرح فاکتوریل بر پایه طرح کاملاً تصادفی در سه تکرار اجرا شد. عوامل آزمایشی شامل دو سطح نانواکسیدروی (2 و 4 میلی‌گرم بر لیتر) و 5 سطح شوری (صفر، 50، 100، 150 و 200 میلی‌مولار) نمک کلرید سدیم در کنار شاهد بود. نتایج حاصل از این تحقیق نشان داد که طول ساقه، وزن‌تر گیاه، تعداد برگ، سطح برگ و میزان رنگیزه‌های فتوسنتزی و همچنین میزان کربوهیدرات‌های محلول  در سطو مختلف شوری کاهش معنی‌داری یافت. از سوی دیگر تیمار 4 میلی‌گرم در لیتر نانواکسیدروی با تأثیر مثبتی بر پارامترهایی رشد،  شاخص پایداری کلروفیل، میزان کلروفیل  و کاروتنوئیدها، پرولین و قندهای محلول برگ و ریشه داشت. همچنین بررسی اثر‌ متقابل داده‌ها نشان داد که بیشترین میزان طول ریشه‌ و وزن‌تر کل در سطح 50 میلی‌مولار کلرید سدیم و نیز میزان پرولین وکلروفیل مربوط به سطح 100 میلی‌مولار کلرید سدیم همراه با محلول‌پاشی نانواکسیدروی با غلظت 4 میلی‌گرم در لیتر بود. به‌طورکلی در این تحقیق مشخص شد مصرف نانواکسیدروی با غلظت 4 میلی‌گرم در لیتر توانست سبب بهبود رشد گیاه و اسمولیت‌های سازگار و در نتیجه بهبود پاسخ گیاه نوروزک به تنش شوری گردد.

کلیدواژه‌ها

: Salvia leriifolia Benth؛ پرولین؛ ‌ تنش شوری؛ شاخص‌های رشد؛ قند محلول؛ ‌کلروفیل نانواکسیدروی

مراجع

Abbasi, A. and Enayati, V. (2013). Decrease of cell defense mechanisms efficiency and oxidative stress accruing in lake of Mg condition. Iranian Journal of Dryland Agriculture, 1 (4): 41-52.

Latef, A.A.H.A., Alhmad, M.F.A., and Abdelfattah, K.E. (2017). The possible roles of priming with ZnO nanoparticles in mitigation of salinity stress in lupine (Lupinus termis) plants. Journal of Plant Growth Regulation, 36(1):60-70.

Abdel-Haliem A.E.F., Hegazy S.H., Hassan N.S. and Naguib D.M. (2017). Effect of silica ions and nano silica on rice plants under salinity stress. Ecological Engineering, 99: 282–289.

Aghaei Joubani, K., Taei, N., Kanani, M.R. and yazdani, M. (2015). Effect of salt stress on some physiological and biochemical parameters of two Salvia species. Journal of Plant Process and Function Iranin Society of Plant Physiology, 3 (9):85-96.

Aghighi Shahverdi, M., Omidi, H. and Tabatabaei, S. (2017). Effect of foliar application of selenium, boron and iron on root morphological and photosynthesis pigments of stevia (Stevia rebaudiana Bertoni) under salinity stress. Journal of Iranian Plant Ecophysiological Research, 33(6):1017-1033.

Alharby, H.F., Metwali, E.M.R., Fuller, M.P. and Aldhebiani, A.Y. (2016). Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress. Archives of Biological Science, 68(4):723-735.

Alpaslan, M., Inal, A., Gunes, A., Cikili, Y. and Oscan, H. (1999). Effect of zinc treatment on the alleviation of sodium and chloride injuryin tomato (Lycopersicon esculentum L.) grown under salinity. Turkish Journal of Botany, 23:1-6.

Arvin, P. (2015). Effect of gibberellin on some morphological traits, photosynthetic pigments content and proline in savory (Satureja hortensis L.) under salinity stress conditions. Journal of Agricultural Research, 7(2): 90-104.

Askary, M., Maghsoudi Moud, A.A. and Saffari, V.R. (2013). Investigation of some physiological characteristics and grain yield of corn (Zea mays L.) hybrids under salinity stress. Journal of Crop Production and Processing Isfahan University of Technology. 3 (9):93-104.

Askary, M., Talebi, Seyed M., Amini, F., Balout, B. and Dousti, A. (2017). Effects of iron nanoparticles on Mentha piperita L. under salinity stress. Biologija. 63:65-75.

Babaei, K., Sharifi, RS., Pirzad, A. and Khalilzadeh, R. (2017). Effects of bio fertilizer and nano Zn-Fe oxide on physiological traits, antioxidant enzymes activity and yield of wheat (Triticum aestivum L.) under salinity stress. Journal of Plant Interactions.12:381–389.

Bates, L.S. (1973). Rapid determination of free proline for water-stress studies. Plant Soil, 39: 205–207.

Ben Taarit, M., Msaada, K., Hosni, K. and Marzouk, B., (2012). Physiological changes and essential oil composition of clary sage (Salvia sclarea L.) resette leaves as affectedby salinity. Acta Physiologiae Plantarum, 33:153-162.

Betran, F.J., Beck, D., Banziger, M. and Edmeades, G.O. (2003). Secondary traits in parental inbreeds and hybrids under stress and nonstress environments in tropical maize. Field Crops Research, 83:51-65.

Broadley, M.R., White, P.J, Hammond, J.P, Zelko, I. and Lux, A. (2007). Zinc in plants. New Phytologist, 173 (4): 677-702.

Chaparzadeh, N., Najjar-Khodabakhsh, A., Pazhang, M. and Zarandi-Miandoab, L. (2015). Effect of salinity and ascorbic acid on growth, water and osmotic relations of Lepidium sativum. Iranian Journal of Plant Biology, 7(24):39-52.

Chaves, M.M., Flexas, J. and Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103: 551-560.

Chinnusamy, V., Jagendorf, A. and Zhu, J.K. (2005). Understanding and improving salt tolerance in plants. Crop Science, 45:437-448.

Croser, C., Renault, S., Franklin, J. and Zwiazek, J. (2001). The effect of salinity on the emergence and seedling growth of Picea morian, Piccea glausa and Pinus banksiana. Environmental Pollution, 115:6-16.

Dashti, M., Kafi, M., Astaraei, A. and Zabihi, H. (2018). Investigation of yield and yield components response of Salvia leriifolia Benth. To the biological and organic Fertilizers. Zeitschrift fur Arznei-und Gewurzpflanzen, 22:84-90.

Dashti, M., Kafi, M., Tavakoli, H. and Mirza, M. (2015)(b). Effect of water deficit on water relation, photosynthesis and osmolytes accumulation of salvia leriifolia Benth. Iranian Journal of Field Crops Research, 12(4):813-821.

Doganlar, Z.B., Demir, K., Basak, H. and Gul, I. (2010). Effects of salt stress on pigment and total soluble protein contents of the three different Tomato cultivars. African Agricultural Journal. 5 (15): 2056-2065.

Dubois, M. and Gilles, K.A. (1956). Colorimetric method for determination of sugar and related substances. Analytical Chemistry, 25(3): 350-354.

Ehdaie, B., Alloush, G.A. Madore, M.A. and Waines, J.G. (2006). Genotypic variation for stem reserves and mobilization in wheat: I. postanthesis changes in internode dry matter. Crop Science, 46: 735- 746.

Ershad Langroudi, M. and Sedaghathoor, S. (2012). Effect of different media and salinity levels on growth traits of Rosemary (Rosmarinus officmali: L.). American-Eurasian Journal of Agriculture and Environment Science, 12 (9): 1134-1142.

Fathi, A., Zahedi, M., Torabian, Sh. and Khoshgoftar, A. (2017). Response of Wheat genotypes to foliar spray of ZnO and Fe nanoparticles under salt stress. Journal of Plant Nutrition, 40(10):1376–1385.

Fazeli Kakhki, S. and Goldani, M. (2018). Effects of Zinc oxide nanoparticles (ZnO) on improving morpho-physiological, yield and its components of Soybean (Glycine max L.) var. Williams under salinity stress. Journal of Crop Ecophysiology (Agriculture Science), 12(46(2)):253-268.

Ghorbanli, M. and Niakan, M. (2005). The effect of drought stress on soluble sugar, total protein, proline, phenolic compound, chlorophyll content and nitrate reductase activity in Soybean (Glycine max L. CV. Gorgan3). Journal of Science (Kharazmi University). 5(1-2): 537-550.

Hendawy, S.F.K. and Khalid, A. (2005). Response of sage Salvia officinalis L. plants to zinc application under different salinity levels. Journal of Applied Sciences Rrsearch. 1: 147-155.

Hosseinzadeh, H., Sadeghnia, H.R., Imenshahidi, M. and Fazly Bazzaz, B.S. (2009). Review of the pharmacological and toxicological effects of Salvia leriifolia. Iranian Journal of Basic Medical Sciences, 12(1):1-8.

Hussein, M.M. and Abou-Baker, N.H. (2018). The contribution of nano-zinc to alleviate salinity stress on cotton plants. Royal Society Open Science, 5: 171-809.

Ayala-Astorga, G. and Alcaraz-Meléndez, L. (2010). Salinity effects on protein content, lipid peroxidation, pigments, and proline in Paulownia imperialis (Siebold & Zuccarini) and Paulownia fortunei (Seemann & Hemsley) grown in vitro. Electronic Journal of Biotechnology, 13(5):1-13.

Jalili, A. and Jamzad, Z. (1999). Red Data Book of Iran .Research Institute of Forest and Rangeland. No. 215.

Jampeetonga, A. and Brix, H. (2009). Effects of NaCl Salinity on Growth, Morphology, Photosynthesis and Proline Accumulation of Salvinia natans Aquatic Botany, (3):181-186.

Kheirizadeh, Y., Seyed Sharifi, R. and Khalilzadeh, R. (2018). Study of biofertilizers and nano zinc oxide application on remobilization and leaf area index of triticale (TriticosecaleWitt.) under soil salinity. Environmental Stresses in Crop Sciences, 11(4): 993-1004.

Kumar, V., Shiram, V., Jawali, N. and Shitole, M.G. (2007). Differential response of indica rice genotypes to NaCl stress in relation to physiological and biochemical parameters. Archives of Agronomy and Soil Science, 53(2):581-592.

Lamas, A., Ullrich, C.I. and Sanz, A. (2002). Cadmium effects on transmembrance electrical potential difference, respiration and membrane permeability of rice (Oryza sativa) roots. Plant and Soil, 219:21-28.

Laware S.L. and Shilpa Raskar. (2014). Influence of Zinc oxide nanoparticles on growth, flowering and seed productivity in Onion. International Journal of Current Microbiology and Applied Science, 3(7):874-881.

Lichtenthaler, H.K. and Wellburn, A.R.  (1983). Determinations of total carotenoids and chlorophylls a and b in leaf extracts in different solvents. Biochemical Society Transactions, 11: 591-592.

Madhan, M.M. (2000). Chlorophyll stability index (CSI): Its impact on salt tolerance in rice. International Rice Research Notes. 25:38-39.

Movahhedi Dehnavi, M., Modarres Sanavi, A.M., Soroush-Zade, A. and Jalali, M. (2004). Changes of proline, total soluble sugars, chlorophyll (SPAD) content and chlorophyll fluorescence in safflower varieties under drought stress and foliar application of zinc and maganese. Biaban, 9 (1): 93-110

Munns, R. (2005). Genes and salt tolerance: bringing them together. New Phytologist, 167: 645 663.

NasirKhan, M. KhorshidAbbas, K.A., AlMutair, H. and Siddiqu, Z. (2016). Role of nanomaterials in plants under challenging environments. Plant Physiology and Biochemistry, 110:194.209.

Niakan, M., Rezapour Mahjoob, S. and Ghorbanli, M. (2015). Effect of exogenous putrescine on growth, photosynthesis and alkaloid compounds of Datura (Datura stramonium L.) in response to salinity stress under hydroponic conditions. Journal of Science and Technology of Greenhouse Culture Soilless Culture Research Center, 6 (1): 111-123.

Ozturk, L., Yazici, M. A., Yucel, C., Torun, A., Cekic, C., Bagci, A., Ozkan, H., Braun, H.J., Sayers, Z. and Cakmak, I. (2006). Concentration and localization of zinc during seed development and germination in wheat. Physiologia Plantarum, 128(1): 144-152.

Pandey, A., Sanjay, S. S. and Yadav, R.S. (2010). Application of ZnO nanoparticlesin influencing the growth rate of Cicer arietinum. Journal of Experiencemental Nanoscience, 5(6): 488-497.

Pardo, J.M. (2010). Biotechnology of water and salinity stress tolerance. Current Opinion in Biotechnology, 21: 185-196.

Parihar, P., Singh, S. and Singh, R. (2015). Effect of salinity stress on plants and its tolerance strategies: a review. Environmental Science and Pollution Research, 22: 40-56.

Rossi, L., Zhang, W., Lombardini, L. and Ma, X. (2016). The impact of cerium oxide nanoparticles on the salt stress responses of (Brassica napus L.). Environmental Pollution, 219:28-36.

Rostami, G., Moghaddam, M., Ghasemi Pirbalouti, A. and Tehranifar, A. (2018). The effects of iron and zinc spraying in sulfate and nano forms on morphological and biochemical properties of peppermint (Mentha piperita L.) under salinity stress. Environmental Stresses in Crop Sciences, 11(3):707-720.

Sajedi, N., Ardakani, M.R. (2006). Effect of different levels of nitrogen, iron and zinc on physiological indices and forage yield of maize (Zea mays L.) in Markazi province. Iranian Journal of Field Crops Research, 6(1): 99-110.

Saxena, R., Tomar, R.S. and Kumar, M. (2016). Exploring Nano biotechnology to mitigate abiotic tress in crop plants. Journal of Pharmaceutical Sciences and Research, 8(9):974-980.

Seyed Sharifi, R., Kamari, H. and Nagafi, Gh. (2014). Effects of salinity stress and foliar application of nano-zinc oxide on yield per plant and some morphophysiological traits of Barley (Hordeum vulgare L.). Iranian Journal of Field Crops Research, 13(2):399-410.

Sim, C.C., Zaharah, A.R., Tan, M.S. and GohRapid, K.J. (2015). Determination of leaf chlorophyll concentration, photosynthetic activity and NK concentration of elaies guineensis via correlated SPAD - 502 chlorophyll index. Asian Journal of Agricultural Research, 9(3): 132-138.

Singh, J., Padmalochan, H. and Jolly, B. (2014). Potential of Vigna unguiculata as a phytoremediation plant in the remediation of Zn from contaminated soil. American Journal of Plant Sciences, 5: 1156-1162.

Singh, M., Kumar, J., Singh, S., Singh, V.P. and Prasad, S.M. (2015). Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review. Reviews in Environmental Science andBio/Technology, 14(3):407-426.

Soleymani, F. and Pirzad, A. (2015). The effect of mycorrhizal fungi on malondialdehyde concentration and some metabolic processes in hyssop (Hyssopus officinalis) under water deficit stress. Iranian Journal of Plant Biology, 7(24):15-26.

Soliman, A. Sh, El-feky, S.A. and Darwish, E. (2015). Alleviation of salt stress on Moringa peregrina using foliar application of nanofertilizers. J. Horticulture and Foresty, 7:36-47.

Talaat, N.B. and Shawky, B.T. (2012). 24-Epibrassinolide ameliorates the saline stress and improves the productivity of wheat (Triticum aestivum L.). Journal Environmental and Experimental Botany, 82:80–88.

Torabian, S., Zahedi, M. and Khoshgoftarmanesh, A. (2016). Effect of foliar spray of zinc oxide on some antioxidant enzymes activity of sunflower under salt stress. Journal of Agricultural Science and Technology, 18 (4):1013-1025.

Torres Netto, A., Campostrini, E., DeOliviera, J.G., and Bressan-Smith, R.E. (2005). Photosynthetic pigments, nitrogen, chlorophyll a fluorescence and SPAD-502 readings in coffee leaves. Scientia Horticulture, 104: 199-209.

Xue, G., Lynne McIntyre, C., Glassop, D. and Shorter, R. (2008). Use of expression analysis to dissect alterations in carbohydrate metabolism in wheat leaves during drought stress. Plant Molecular Biology, 67: 197–214.

Yadarlou, L. and Majidi Heravan, E. (2008). Evaluation of salinity stress on morphophysiological traits of four salinity tolarant wheat cultivars. Iranian Journal of Field Crops Research, 1:205-215.

Yadghari, R., Nyakan, M. and Mosavat, A. (2014). The effect of nano and non-nano forms chelate zinc on growth, chlorophyll content and soluble sugar pea plants (Cicer arietinum L.) in different levels of salinity. Iranian Journal of Plant Ecophysiology Research. 9:137-150.