اخبار و اعلانات

 آمار

تعداد نشریات418
تعداد شماره‌ها9,997
تعداد مقالات83,560
تعداد مشاهده مقاله77,801,170
تعداد دریافت فایل اصل مقاله54,843,829
Kotova, Anastasiya V., Kovtun, Oleg A., Podgornaya, Olga I., Adonin, Leonid S.. (1394). Mesoglein differences between two jellyfish populations of the genus Aurelia. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 7(2), 123-131. doi: 10.1007/s40071-015-0096-8
Anastasiya V. Kotova; Oleg A. Kovtun; Olga I. Podgornaya; Leonid S. Adonin. "Mesoglein differences between two jellyfish populations of the genus Aurelia". نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 7, 2, 1394, 123-131. doi: 10.1007/s40071-015-0096-8
Kotova, Anastasiya V., Kovtun, Oleg A., Podgornaya, Olga I., Adonin, Leonid S.. (1394). 'Mesoglein differences between two jellyfish populations of the genus Aurelia', نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 7(2), pp. 123-131. doi: 10.1007/s40071-015-0096-8
Kotova, Anastasiya V., Kovtun, Oleg A., Podgornaya, Olga I., Adonin, Leonid S.. Mesoglein differences between two jellyfish populations of the genus Aurelia. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 1394; 7(2): 123-131. doi: 10.1007/s40071-015-0096-8

Mesoglein differences between two jellyfish populations of the genus Aurelia

International Aquatic Research
مقاله 4، دوره 7، شماره 2، شهریور 2015، صفحه 123-131    اصل مقاله (1.23 M)
شناسه دیجیتال (DOI): 10.1007/s40071-015-0096-8
نویسندگان
Anastasiya V. Kotova1؛ Oleg A. Kovtun2؛ Olga I. Podgornaya1، 3؛ Leonid S. Adonin* 1، 3
1Institute of Cytology RAS, St. Petersburg, Russia
2Hydrobiological Station of Odessa National I. I. Mechnikov University, Odessa, Ukraine
3Department of Cytology and Histology, Faculty of Biology and Soil Sciences, St-Petersburg State University, St.Petersburg, Russia
چکیده
The medusa, Aurelia aurita (Scyphozoa, Cnidaria), is counted as a cosmopolitan species with a worldwide distribution in most seas, from the poles to the tropics. Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by huge mesoglea in medusa. The main medusas’ morphology is similar in different populations. Previously the new protein “mesoglein” was determined as one of the main components of mesoglea. Deduced amino acid sequence of mesoglein contains Zona Pellucida (ZP) domain. The comparison of mesoglein and its gene from three habitats White Sea (WsA), Black Sea (BsA), Japonic Sea (JsA) has been done in the current work. The set of the mesoglea protein bands after SDS-PAGE is similar in all samples. Nevertheless, JsA mesogleins’ M r is 53\55 kDa, while WsA and BsA mesogleins have M r of 47 kDa. Antibodies raised against WsA mesoglein recognize only mesoglein with M r of 47 kDa, but not 53\55 kDa, both on immunoblot and immunocytochemistry. Mesogleal cells and “elastic” fibrils are stained intensively in the mesoglea both from WsA and BsA but not from JsA. The possibility of gene diversity was checked by PCR with primers specific for WsA mesoglein gene. PCR products of expected length were obtained on cDNA from polyA-RNA template from mesogleal cells of WsA and BsA medusa but not on cDNA of JsA medusa. Our results evidence that there are two different species in genus Aurelia: Aurelia aurita inhabits White and Black Seas while Aurelia sp.1 inhabits Japonic Sea. Such a suggestion is in agreement with the one previously done on the base of comparison by other molecular biology methods.
کلیدواژه‌ها
Aurelia aurita؛ Jellyfish؛ Mesoglein؛ Evolution
آمار
تعداد مشاهده مقاله: 130
تعداد دریافت فایل اصل مقاله: 73


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب