Evaluation of the effect of ultrasound-assisted variables on phenolic compounds extraction from almond(Prunus amygdalus) hull

Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

مقاله 4، دوره 10، شماره 2، مهر 2019، صفحه 23-33   

نوع مقاله: Original article

نویسندگان

Victoria Masoudi¹؛ Aazam Aarabi^{* 2}

¹Department of Chemical Engineering, Shahreza Branch, Islamic Azad University, Shahreza, Iran

²Department of Food Science and Technology, Shahreza Branch, Islamic Azad University, Shahreza, Iran

چکیده

Background & aims: Extraction of major phenolic compounds from agricultural crop residues is important for the development of value-added products. Almond (Prunus amygdalus) is one of the most indigenous fruit crops that is grown in large quantities in Iran. Almond hulls are considered as by-product which are used only for animal feed. The aim of this study was to compare the phenolic compounds extraction yield from almond hulls using ultrasound and percolation methods.
Experimental: An optimization procedure using central composite design (CCD) with 4 factors (solvent, frequency, time and temperature) at 5 levels was used in order to investigate the effect of these parameters on the extraction. Finally, the effect of solid to solvent ratio on phenolic compounds extraction was evaluated and compared with soxhlet method.
Results: The results indicated that the optimum extraction conditions is found to be 86% ethanol, 50 °C , 75 min and 163 Hz  for  temperature,  time and frequency, respectively. In addition, the extraction of phenolic compounds was evaluated in different ratios of solid to solvent (10: 1, 20: 1, 30: 1, 40: 1(w / w) at the optimum conditions and antioxidant activity of the extracts was measured by DPPH method. The maximum extraction of phenolic compounds was obtained in the ratio of 40: 1(w / w) with the amount of 1134.19± 0.23 mg/g of dry matter. The maximum free radical scavenging activity of the extract was 87.42% for ultrasound extraction which was significantly (α<0.05) different from percolation method.
Recommended applications/industries: Application of new techniques including ultrasound in the extraction of phenolic compounds can be an effective method not only to increase the extraction yield of phenolic compounds but also due to the shorter extraction time, less damage is done to these compounds.

کلیدواژه‌ها

Almond (Prunus amygdalus)؛ Phenolic compounds؛ Ultrasound؛ Antioxidant capacity

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