تاثیر سالیسیلیک اسید بر برخی خصوصیات فیزیولوژیکی و تولید اسانس به‌لیمو (Lippia citrodora L.) تحت تنش شوری

فیزیولوژی محیطی گیاهی

دوره 17، شماره 65، فروردین 1401، صفحه 54-65   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2021.679535

نویسندگان

محمد مقدم^{* 1}؛ لیلا مهدی زاده²؛ سارا فرسرایی³

¹گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران.

²گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران

³گروه علوم باغبانی و مهندسی فضای سبز، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد ایران

چکیده

هدف از این تحقیق مطالعه اثر کاربرد سالیسیلیک اسید بر برخی از خصوصیات فیزیولوژیکی و تولید اسانس گیاه دارویی به لیمو (Lippia citrodora L.) تحت تنش شوری بود. بدین منظور آزمایشی گلدانی بصورت فاکتوریل بر پایه طرح کاملاً تصادفی با 4 سطح شوری (0، 50، 100 و 150 میلی‏مولار کلرید سدیم) و 4 سطح سالیسیلیک اسید (0، 150، 300 و 450 میلی‏گرم در لیتر) در 3 تکرار انجام شد. صفات اندازه‏گیری شده شامل میزان پروتئین محلول کل، مالون‏دی‏آلدئید، پراکسید هیدروژن، گلایسین بتائین و فعالیت برخی آنزیم‏های آنتی‏اکسیدانی و تولید اسانس بودند. اثر متقابل تیمارهای آزمایش بر تمام صفات مورد مطالعه در این تحقیق در سطح احتمال یک درصد معنی‏دار شد. نتایج این تحقیق نشان داد که بیشترین میزان پروتئین محلول کل، مالون‏دی‏آلدئید، پراکسید هیدروژن، گلایسین بتائین و فعالیت آسکوربات پراکسیداز و سوپراکسید دیسموتاز در سطح 150 میلی‏مولار شوری بود و کاربرد سالسیلیک اسید در سطح 300 میلی‏گرم در لیتر سبب کاهش پروتئین محلول کل و گلایسین بتائین و سطح 150 میلی‏گرم در لیتر آن سبب کاهش مالون‏دی‏آلدئید شد. اما کاربرد این تنظیم‌کننده رشد گیاهی تأثیر معنی‏داری بر پراکسید هیدروژن نداشت. همچنین، نتایج نشان داد که فعالیت آنزیم کاتالاز و تولید اسانس در بالاترین سطح تنش شوری (150 میلی‏مولار) به کمترین میزان خود رسیدند. نتایج این تحقیق در مجموع نشان داد که کاربرد سالیسیلیک اسید به‏ویژه در سطح 300 میلی‏گرم در لیتر سبب بهبود صفات مورد مطالعه در این تحقیق گردید و کاربرد آن در شرایط تنش توصیه می‏شود.

کلیدواژه‌ها

آسکوربات پراکسیداز؛ سوپراکسید دیسموتاز؛ کاتالاز؛ گلایسین بتائین؛ مالون‏دی‏آلدئید

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