بررسی تاثیر محلول پاشی اسید آسکوربیک بر فعالیت آنزیم های آنتی اکسیدان ارقام ماش (Vigna radiate L.) در شرایط تنش کم آبی

فیزیولوژی محیطی گیاهی

دوره 17، شماره 66، تیر 1401، صفحه 126-140   

نوع مقاله: پژوهشی

شناسه دیجیتال (DOI): 10.30495/iper.2021.679565

نویسندگان

محمد جهانبخشی؛ مهدی صادقی^* ؛ محمود توحیدی؛ فربد فتوحی؛ سیدعلی فاضل زاده

گروه زراعت، واحد دزفول، دانشگاه آزاد اسلامی، دزفول، ایران.

چکیده

به‌منظور بررسی اثر محلول‌پاشی آسکوربیک اسید بر فعالیت آنزیم‌های آنتی‌اکسیدان ژنوتیپ های ماش (Vigna Radiate L.) در شرایط تنش کم‌آبی، آزمایشی مزرعه‌ای به‌صورت اسپلیت فاکتوریل در قالب طرح بلوک­های کامل تصادفی در سه تکرار طی دو سال زراعی 1396 و 1397 در مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی صفی‌آباد دزفول به اجرا در آمد. تیمارهای تنش کم‌آبی در کرت‌های اصلی شامل چهار سطح (25، 50، 75 و 100 درصد تأمین نیاز آبی گیاه) و تلفیق تیمار محلول­پاشی آسکوربیک اسید شامل 3 سطح (شاهد، 10 و 20 میلی مولار) و ژنوتیپ‌های امیدبخش CV 6173 و پرتو در کرت‌های فرعی بود. نتایج مقایسه میانگین نشان داد که بیشترین میزان فعالیت آنزیم سوپراکسیددیسموتاز و آسکوربات پراکسیداز در تنش کم آبی 25 درصد نیاز آبی و عدم محلول‌پاشی بود. بیشترین فعالیت آنزیم کاتالاز تحت تنش کم آبی 50 درصد نیاز آبی و تیمار عدم محلول‌پاشی آسکوربیک اسید مشاهده شد. همچنین بیشترین فعالیت آنزیم گایاکول پراکسیداز در رقم امید بخش VC6173 تحت تنش کم آبی 25 درصد نیاز آبی و عدم محلول‌پاشی آسکوربیک اسید بدست آمد بیشترین میزان فعالیت آنزیم گلوتاتیون پراکسیداز نیز به رقم امیدبخش VC6173 تحت تنش کم آبی 75 درصد نیاز آبی و محلول‌پاشی 10 میلی مولار آسکوربیک تعلق داشت.به طور کلی نتایج این تحقیق نشان داد که تنش کم آبی منجر به افزایش معنی دار فعالیت آنزیم‌های آنتی اکسیدان شد و محلول پاشی آسکوربیک‌اسید به دلیل خاصیت آنتی‌اکسیدانی خود منجر به کاهش اثرات تنش و در نتیجه کاهش فعالیت آنزیم های آنتی اکسیدان گشت.

کلیدواژه‌ها

آسکوربیک اسید؛ آسکوربات پراکسیداز؛ تنش کم آبی؛ سیستم آنتی اکسیدان؛ کاتالاز؛ گلوتاتیون پراکسیداز؛ محلول پاشی

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