In-vitro propagation of Pluchea lanceolata (DC) C.B.Clarke a potent antiarthritic medicinal herb through axillary bud

Trends in Phytochemical Research

دوره 5، شماره 1، خرداد 2021، صفحه 24-30   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/tpr.2021.680483

نویسندگان

Satendra Singh^{* 1، 2}؛ Ritu Mishra^{1، 3}؛ Rajneesh K. Agnihotri²

¹Division of Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow -226015 (U.P.), India

²Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002(U.P.), India

³Academy of Scientific and Innovative Research (AcSIR), CSIR-Human Resource Development Centre Campus, Sector-19, Kamla Neh Nagar, Ghaziabad-201002 (U.P.), India

چکیده

Pluchea lanceolata, commonly known as Rasana, is highly valued for its anti-inflammatory, antiarthritic, and analgesic properties and is extensively employed in treating rheumatoid arthritis, cough, neurological diseases, edema, sciatica, psoriasis, bronchitis, dyspepsia, and piles. Shrinking populations and insufficient variation due to rootstock propagation methods warrant efficient mass multiplication and conservation protocols. In the present study, explant (axillaries bud was cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxin (PGRs) individually, as well as in combination. The highest mean shoot number (2.06 ± 1.43) and second highest mean shoot length (2.62 ± 1.49 cm) were obtained in MS media enriched with 4 mg/L Benzyl amino purine (BAP) + 1mg/L Naphthalene acetic acid (NAA). Whereas, the best shoots multiplication (2.62 ± 1.41) and shoots elongation (5.88 ± 2.44) responses were displayed when MS medium supplemented with 0.25 mg/L (BAP) +0.50 mg/L Kinetin (KIN) were used.
 

کلیدواژه‌ها

Axillaries bud explant؛ In-vitro propagation؛ MS medium؛ PGRs؛ Pluchea lanceolata

مراجع

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