Effect of Foliar Application of Proline on Morphological and Physiological Traits of Calendula officinalis L. under Drought Stress

Journal of Ornamental Plants

مقاله 2، دوره 11، شماره 1، خرداد 2021، صفحه 13-30   

نوع مقاله: Research Paper

نویسندگان

Sophia Soroori¹؛ Elham Danaee^{* 2}؛ Khodayar Hemmati³؛ Alireza Ladan Moghadam⁴

¹Ph.D. Student, Medicinal Plant, Department of Horticultural Sciences, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran

²Assistant Professor, Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran

³Associate Professor, Department of Horticulture Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

⁴Associate Professor, Department of Horticultural Sciences, Garmsar Branch, Islamic Azad University, Garmsar, Iran

چکیده

Drought stress is one of the most important abiotic stresses that seriously reduces the production of ornamental plants such as pot marigold. Proline is one of the active amino acids in the osmotic regulation phenomenon and it has an effective role in reducing cell damage and improving drought tolerance. A pot experiment as factorial arrangement in a completely randomized design with three replications was performed in order to study the effect of foliar application of proline (0, 50 and 100 mg L^-1) on some morpho- physiological characteristics of pot marigold under drought stress (normal irrigation, 25, 50 and 75 % field capacity). The results showed that the highest fresh and dry weight of aerial organ, longevity of the flower on the plant, carotenoid and total chlorophyll were observed in normal irrigation+ proline 100 mg L^-1. The highest fresh and dry root weight, root length and total antioxidant activity (DPPH) were for 75 % FC + proline 100 mg L^-1. The highest plant height and number of flowers were in normal irrigation+ proline 50 mg L^-1. The highest proline content was belonged to 25 % FC+ proline 100 mg L^-1. In general, the results of this experiment showed that application of proline can partially compensate for the adverse effects of dehydration and on the other hand, despite the fact that the pot marigold plant is always able to survive in drought stress, it is recommended to irrigate 75% FC in order to increase pot marigold performance.

کلیدواژه‌ها

Antioxidant activity؛ Carotenoid؛ Chlorophyll؛ Field capacity؛ Plant longevity

مراجع

Abdossi, V. and Danaee, E. 2018. Effects of some amino acids and organic acids on enzymatic activity and longevity of Dianthus caryophyllus cv. Tessino at pre-harvest stage. Journal of Ornamental Plants, 9 (2): 93-104. 

Ali, Q., Anwar, F., Ashraf, M., Saari, N. and Perveen, R. 2013. Ameliorating effects of exogenously applied proline on seed composition, seed oil quality and oil antioxidant activity of maize (Zea mays L.) under drought stress. International Journal of Molecular Sciences,14 (1): 818-835.

Ali, Q., Ashraf, M. and Athar, H.R. 2007. Exogenously applied proline at different growth stages enhances growth of two maize cultivars grown under water deficit conditions. Pakistan Journal of Botany, 39 (4): 1133-1144.

Alipour, S., Farahmand, F. and Nasibi, F. 2016. Influence of proline treatment on some physiological morphological characteristics and postharvest life of cut tuberose (Polianthes tuberosa L.). Plant Process and Function, 4 (14): 114-106.

Ashraf, M. and Foolad, M.R. 2007. Roles of glycine betaine and proline in improving plant abiotic stress tolerance. Environmental and Experimental Botany, 59 (2): 206- 216.

Baghalian, K., Abdoshah, S.H., Khalighi-Sigaroodi, F. and Paknejad F. 2011. Physiological and phytochemical response to drought stress of German chamomile (Matricaria recutita L.). Plant Physiology and Biochemistry, 49 (20): 201-207.

Barnes, J.D., Balaguer, L., Manrique, E., Elvira, S. and Davison, A.A. 1992. A reappraisal of the use of DMSO for extraction and determination of chlorophyll a and b in lichens and higher plants. Environmental and Experimental Botany, 32: 85-100.

Bates, L.S., Waldran, R.P. and Teare, I.D. 1973. Rapid determination of free proline for water studies. Plant Soil, 39: 205-207.

Buntariu, M. and Zepa Cradini, C. 2012. Evaluation of biologically active compounds from Calendula officinalis flowers using spectrophotometry. Butnariu and Coracini Chemistry Central Journal, 6 (35): 1-7.

Chen, C. and Dickman, M. B. 2005. Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii. Proceedings of the National Academy of Sciences. United States of America, 102 (9): 3459-3464.

Danaee, E. and Abdossi, V. 2018. Effect of different concentrations and application methods of polyamines (putrescine, spermine, spermidine) on some morphological, physiological, and enzymatic characteristics and vase life of Rosa hybrida cv. ‘Dolce Vita’ cut flower. Journal of Ornamental Plants, 8 (3): 171-182.

Darvizheh, H. and Zavareh. M. 2018. The effect of proline foliar application on reducing the effects of dehydration stress on German chamomile (Matricaria chamomilla L). Ecological Quarterly Journal of Crop Plants, 14 (1): 43-33. (In Persian)

Darvizheh, H., Zavareh, M. and Ghasmanjad, M. 2017. Effect of proline spraying on biochemical properties of German chamomile in water stress conditions (Matricaria chamomilla L). Journal of Applied Research in Plant Ecophysiology, 4 (1): 35-60. (In Persian)

Dawood, M.G., Taie, H.A.A., Nassar, R.M.A., Abdelhamid, M.T. and Schmidhalter, U. 2014. The changes induced in the physiological, biochemical and anatomical characteristics of Vicia faba by the exogenous application of proline under seawater stress. South African Journal of Botany, 93: 54–63.

Deivanai, S., Xavier, R., Vinod, V., Timalata, K. and Lim, O.F. 2011. Role of exogenous proline in ameliorating salt stress at early stage in two rice cultivars. Journal of Stress Physiology and Biochemistry, 7 (4): 157-174.

Dolatkhahi, A., Matloobi, M., Motallebiazar, A. and Vahdati, N. 2013. Shading impact on qualitative characteristics and chlorophyll content of cut rose (Rosa hybrida cv. Avalanche). Journal of Ornamental Plants, 3 (4): 215-220.

Dulai, S., Molnar, I., Pronay, J., Csernak, A., Tarnai, R. and Molnar-Lang, M. 2006. Effects of drought on photosynthetic parameters and heat stability of PSII in wheat and in Aegilops species originating from dry habitats. Acta Biologica Szegediensis, 50 (1-2): 11-17.

Ezhilmathi, K., Singh, V., Arora, P. and Sairam, R.K. 2007. Effect of 5-sulfocalicylic acid on antioxidant in relation to vase life of gladiolus cut flower. Plant Growth Regulation, 51 (2): 99-108.

Farooq, M., Wahid, A., Kobayashi, N., Fujita, D. and Basra, S.M.A. 2009. Plant drought stress effects, mechanisms and management. Agronomy for Sustainable Development, 29 (1): 185-212.

Foyer, C. and Spancer, C. 1986. The relationship between phosphate status and photosynthesis in leaves effects on intracellular orthophosphate distribution photosynthesis and assimilate partitioning. Planta, 167 (3): 369-375.

Ganjeali, A. and Bagheri, A. 2010. Evaluation of morphological characteristics of root chickpea (Cicer arietinum L.) in response to drought stress. Iranian Journal of Pulses Research, 1 (2):101-110.

Gholami Zali, A. and Ehsanzadeh, P. 2018. Exogenous proline improves osmoregulation, physiological functions, essential oil, and seed yield of fennel. Industrial Crops and Products, 111: 133–140.

Habibi, D., Ardakani, M.R., Mahmoudi, A. and Asgharzadeh, A. 2010. Effects of super absorbent polymer and plant growth promoting rhizobacteria (PGPR) on yield and oxidative damage of maize under drought stress. International Conference on Chemistry and Chemical Engineering. Kyoto, Japan. 1-3 August, 253-257.

Hamidipour, P., Amoaghaei, R. and Bahreininejad, B. 2017. Effect of aminolevulinic acid on growth, essential oil content and antioxidant responses in Mentha piperita under drought stress. M.Sc. Thesis. Shahrekord University. (In Persian)

Hasanuzzaman, M., Nahar, K., Gill, S.S. and Fujita, M. 2013. Drought stress responses in plants, oxidative stress, and antioxidant defense. Climate Change and Plant Abiotic Stress Tolerance, First Edition. Wiley-VCH Verlag GmbH and Co. KGaA. Part 9, pp. 209- 249.

Hashem, A., Abd Allah, E.F., Alqarawi, A.A. and Egamberdieva, D. 2015. Bioremediation of adverse impact of cadmium toxicity on Cassia italica Mill. by arbuscular mycorrhizal fungi. Saudi Journal of Biological Sciences, 23 (1): 39-47.

Hashemabadi, D., Asadi Beigzadeh-Mahaleh, Z. and Pourzarnegar, F. 2019. Morphological and physiological traits of Catharanthus roseus L. at different irrigation intervals as affected by salicylic acid application. Journal of Ornamental Plants, 9 (3): 165-178.

Hassanzadeh, K., Hemmati, Kh. and Mehdipour, M. 2018. The effect of different drying methods (natural and aven) on the drying time and some secondary metabolites of the medicinal plant lemongrass (Melissa officinalis L.). Journal of Plant Production Research, 25 (1): 137-143. (In Persian)

Hassanzadeh Fard., Sh. and Arvin, M.J. 2013. The role of glycine betaine and proline in increasing drought resistance with emphasis on its functional aspects. The First National Conference on Agricultural Sciences with an Emphasis on Non-biological Stresses. Payame Noor Naghadeh University. 18-19 September. https://www.civilica.com/Paper-NCASPNU01-NCASPNU01_079.html.

Hatamifar, N. and Babadaei Samani, R. 2017. Effect of paclobutrazol on some morphological and physiological characteristics of petunia under drought stress. Journal of Ornamental Plants, 7 (2): 125-136.

Hossaina, Z., Kalam, A., Mandala, A., Kumar Dattaa, S. and Krishna Biswasb, A. 2005. Decline in ascorbate peroxidase activity –A prerequisite factor for tepal senescence in Gladiolus. Journal of Plant Physiology, 163 (2): 186-194.

Idrees, M., Khan, M. M. A., Aftab, T., Naeem, M. and Hashmi, N. 2010. Salicylic acid induced physiological and biochemical changes in lemongrass varieties under water stress. Journal of Plant Interactions, 5 (4): 293-303.

Islam, M.M., Hoque, M.A., Okuma, E., Banu, V., Shimoishi, Y. and Nakamura, Y., 2009. Exogenous proline and glycinebetaine increase antioxidant enzyme activities and confer tolerance to cadmium stress in cultured tobacco cells. Journal of Plant Physiology, 166 (15):1587-97.

Jalali, F. and Naderi, D. 2019. Study of morphological and biochemical traits of marigold as influenced by phosphorous biofertilizer and zinc. Journal of Ornamental Plants, 9 (4): 275-290.

Karima, M., Gamal, E.D. and Abdel-Wahed, M.S.A. 2005. Effect of some amino acids on growth and essential oil content of chamomile plant. International of Journal of Agriculture and Biology, 7 (3): 376-380.

Kashefi, B. and Bahri, F. 2019. The effect of drought stress and hexaconazole on physio-morphological characteristics of Malva sylvestris L. Environmental Stress in Crop Sciences, 12 (3): 877-887. (In Persian)

Kaya, C., Levent Tuna, A., Ashraf, M. and Altunlu, H. 2007. Improved salt tolerance of melon (Cucumis melo L.) by the addition of proline and potassium nitrate. Environmental and Experimental Botany, 60 (3): 397–403.

Khalil Soha, E. and El-Noemani, A.A. 2012. Effect of irrigation intervals and exogenous proline application in improving tolerance of garden cress plant (Lepidium sativum L.) to water stress. Journal of Applied Sciences Research, 8 (1):157-167.

Kocheva, K. and Georgive, G .2003. Evaluation of the reaction of two contrasting barley (Hordeum vulgare L.) cultivars in response to osmotic stress with PEG6000. Bulgarian Journal of Plant Physiology, 1 (1): 290-294.

Kumar, N., Pal, M., Singh, A., Sairam R.K. and Srivastava, G.C. 2010. Exogenous proline alleviates oxidative stress and increase vase life in rose (Rosa hybrida L. ‘Grand Gala’). Scientia Horticulture, 127 (1): 79–85.

Lama, B., Ghosal, M., Gupta, S. and Mandal, P. 2013. Assessment of different preservative solutions on vase life of cut roses. Journal of Ornamental Plants, 3 (3): 171-181.

Lawlor, D.W. 2002. Limitation to photosynthesis in water stressed leaves: Stomata versus metabolism and the role of ATP. Annals of Botany, 89 (7): 871-85.

Maiato, H., DeLuca, J., Salmon, E. and Earnshaw, W.C. 2004. The dynamic kinetochore-microtubule interface. Journal of Cell Science, 117 (23): 5461-5477.

Mansouri Motehaver, M., Mosavi Fard, S. and Rezaeinejad, A. 2019. The effect of proline application on the morphophysiological characteristics of Catharanthus roseus salinity stress. M.Sc. Thesis, Faculty of Agriculture, Physiology and Flower and Ornamental Plant Breeding, Lorestan University. (In Persian)

Mirzaee Esgandian, N., Jabbarzadeh, Z. and Rasouli Sadaghiani, M.H. 2020. Investigation on some morphological and physiological characteristics of Gerbera jamesonii as affected by humic acid and nano-calcium chelate in hydroponic culture conditions. Journal of Ornamental Plants, 10 (1): 1-13.

Misra, A. and Srivastava, N.K. 2000. Influence of water stress on Japanese mint. Journal of Herbs, Spices and Medicinal Plants, 7 (1): 51-58.

Mohammadi Torkashvand, A., Misaghi, M. and Ramezanzadeh Bisheh Gahi, F. 2015. The effect of growth bed containing organic substrates and irrigation period on the growth of Araucaria excelsa L. Journal of Ornamental Plants, 5 (4): 231-238.

Mostofi, Y. and Najafi, F. 2005. Analytical laboratory methods in horticultural sciences. Translation of Tehran University Press. (In Persian)

Moustakas, M., Sperdouli, I., Kouna, T., Antonopoulou, C. I. and Therios, I. 2011. Exogenous proline induces soluble sugar accumulation and alleviates drought stress effects on photosystem II functioning of Arabidopsis thaliana leaves. Journal of Plant Growth Regulation, 65 (2): 315–325.

Naseri Moghadam, A., Bayat, H., Aminifard, M. and Moradi Nezhad, F. 2019. Effect of drought and salinity stress on growth, flowering and biochemical characteristics of Narsicuss tazetta L. Journal of Horticultural Sciences, 33 (3): 466-451. (In Persian)

Okuma, E., Murakami, Y., Shimoishi, Y., Tada, M. and Murata, Y. 2004. Effects of exogenous application of proline and betaine on the growth of tobacco cultured cells under saline conditions. Soil Science and Plant Nutrition, 50 (8): 1301-1305.

Oraee, T., Shoor, M., Tehranifar, A. and Nemati, H, 2019. Alleviation of drought stress effects by exogenous application of spermidine and salicylic acid on hollyhock (Alcea rosea). Journal of Ornamental Plants, 9 (4): 303-318.

Pal, M., Szalai, G. and Janda, T. 2015. Polyamines are important in abiotic stress signaling. Plant Science, 237: 16-23.

Pei, F., Li, X., Liu, X. and Lao, C. 2013. Assessing the impacts of droughts on net primary productivity in China. Journal of Environmental Management, 114: 362–371.

Ranjan, R., Bohra, S.P. and Jeel, A.M. 2001. Book of plant senescence. Jodhpur, Agrobios New York. pp 18- 42.

Roozbehani, F., Mosavi Fard, S. and Rezaeinejad, A. 2018. The effect of proline on some morphophysiological, physiological and biochemical properties of two varieties of Impatiens walleriana under salinity stress. M.Sc. thesis. University of Lorestan. (In Persian)

Saxena, S., Kaushik, N. and Sharma, R. 2008. Effect of abscisic acid and proline on in vitro flowering in Vigna aconitifolia. Biologia Plantarum, 52 (1): 181-190.

Shakarami, Kh., Zahedi, B., Rezaei Nejad, A. and Mousavi Fard, S. 2019. Evaluation of physiological responses of tuberose (Polianthes tuberosa L.) to water deficit stress. Journal of Ornamental Plants, 9 (4): 259-273.

Sharkey, T.D., Carl, J.B., Graham, D.F. and Singsaas, E.L. 2007. Fitting photosynthetic carbon dioxide response curves for C₃ leaves. Plant Cell Environment, 30 (9): 1035-1040.

Sodaiezadeh, H., Shamsayi, M., Tajamolian, M., Mirmohammadi Meybodi, A. and Hakimzadeh, M. 2016. The effect of drought stress on some morphological and physiological traits of Satureja hortensis. Plant Process and Function, 5 (15): 1-11.

Spinardi, A.M. 2005. Effect of harvest date and storage on antioxidant systems in pears. Acta Horticulture, 682: 655-662.

Szabados, L. and Savoure, A. 2009. Proline: A multifunctional amino acid. Trends in Plant Sciences, 15 (2): 89-97.

Tian, Z., Wang, F., Zhang, W., Liu, CH. and Zhao, X. 2012. Antioxidant mechanism and lipid peroxidation patterns in leaves and petals of marigold in response to drought stress. Horticulture, Environment and Biotechnology, 53:183-193.

Zamani, S., Kazemi, M. and Aran, M. 2011. Postharvest life of cut rose flowers as affected by salicylic acid and glutamine. World Applied Science Journal, 12 (9): 1621- 1624.

Zarinkolah, M., Shamshiri, M., Karimi, H. and Esmailizadeh, M. 2013. Effect of irrigation levels and exogenous proline application in improving tolerance of ironwood seedlings (Parrotia persica) to drought stress. M.Sc. Thesis, Vali-e-Asr University of Rafsanjan. (In Persian)

Zekavati, H., Shoor, M., Rohani, H., Fazeli Kakhki, F. and Ganji Moghadam, A. 2019. Effect of Trichoderma fungus (Separation 65) on morphological and biochemical characteristics of sage under drought stress. Crops Improvement, 21 (1): 62-72. (In Persian)