اثر هشت هفته تمرین استقامتی همراه با مصرف عصاره پسته وحشی (بنه) بر بیان ژن گرلین بافت روده موش های صحرایی

تحقیقات در علوم ورزشی و گیاهان دارویی

دوره 1، شماره 2، اسفند 1399، صفحه 26-36   

نوع مقاله: مقاله پژوهشی

شناسه دیجیتال (DOI): 10.30495/varzesh.2021.1919423.1005

نویسنده

صالح رحمتی احمدآباد^*

گروه تربیت‌بدنی، واحد پردیس، دانشگاه آزاد اسلامی، پردیس، ایران

چکیده

مقدمه و هدف: بر هم خوردن تعادل انرژی موجب بروز بیماری­های مختلفی مانند چاقی، دیابت و غیره می­شود. پپتیدهای زیادی در تنظیم تعادل انرژی در بدن نقش دارند که گرلین یکی از مهم­ترین آن­ها است. این مطالعه اثر یک دوره تمرین استقامتی و عصاره پسته وحشی (بنه) (به‌عنوان گیاهی غنی از اسیدهای چرب)، بر بیان ژن گرلین بافت روده موش‌های صحرایی ماده را بررسی می­نماید.
مواد و روش ها: در این مطالعه تجربی 20 سر موش صحرایی ماده بالغ نژاد ویستار انتخاب و به‌طور تصادفی به چهار گروه 1) کنترل سالین، 2) تمرین سالین، 3) کنترل بنه و 4) تمرین + بنه تقسیم شدند. گروه‌های 2 و 4 به مدت هشت هفته (پنج روز در هفته، هر جلسه به مدت 60 دقیقه و سرعت 25 متر بر دقیقه با شیب صفر) روی نوارگردان مخصوص جوندگان به تمرین پرداختند در حالی که گروه­های 1 و 3 تمرین نداشتند. گروه‌های 3 و 4 عصاره پسته وحشی و گروه‌های 1 و 3 محلول سالین را قبل از تمرین به صورت گاواژ دریافت می‌کردند (یک میلی­گرم به ازای هر گرم وزن بدن). 72 ساعت پس از آخرین جلسه­ی تمرینی در وضعیت ناشتایی، موش­های صحرایی بیهوش شده و بافت‌برداری انجام شد. داده‌ها با استفاده از آزمون آنالیز واریانس دو‌طرفه آنالیز شدند و 05/0> p معنی‌دار در نظر گرفته شد.
یافته ­ها: هشت هفته تمرین هوازی منجر به کاهش بیان ژن گرلین در بافت روده موش­های صحرایی شد (001/0> p ) اما بیان آن در پاسخ به استفاده از پسته وحشی افزایش یافت (001/0> p ).
بحث و نتیجه گیری: استفاده از رژیم غذایی پرچرب (عصاره بنه) باعث افزایش بیان ژن گرلین بافت روده شد. انجام هشت هفته تمرین هوازی این افزایش را تعدیل نمود.

کلیدواژه‌ها

گرلین؛ پسته وحشی (بنه)؛ تمرین هوازی؛ روده

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