Effect of Auxin derivatives on morphological and isoenzyme pattern of enzymatic Antioxidant peroxidase (POX) of “blinding eye mangrove” Excoecaria agallocha.L stem cuttings.

Iranian Journal of Plant Physiology

دوره 11، شماره 2، فروردین 2021، صفحه 3571-3578   

نوع مقاله: Original Article

شناسه دیجیتال (DOI): 10.30495/ijpp.2021.1878456.1162

نویسندگان

Ramamurthy Somasundaram^{* 1}؛ Roseline Jebapriya²؛ REYAZ AHMAD MIR³

¹Botany, Science, Annamalai University, Chidambaram, TamilNadu India, 608 002

²Department of Botany, Annamalai University, Annamalai Nagar-608 002, Tamil Nadu, India

³Department of Botany Annamalai University TamilNadu-India

چکیده

In our investigation, effect of auxin on rooting and sprouting behavior of stem cutting of Excoecaria agallocha has been studied. Initially stem cuttings was pretreated to remove the phenol content in the cutting and then the stem cuttings that was devoid from phenols cuttings were subjected to hormonal treatment with auxins derivatives as IBA 2000ppm, IPA 2000ppm, NAA 2000ppm and IBA+NAA combination 2000ppm. The root length and their number, rooting and sprouting percentage, number of leaves per cutting, leaf area and photosynthetic pigments were analyzed on 40, 50 and 60 day after planting(DAP) and isoenzyme pattern of peroxidase of root and leaf were also analyzed. Among the auxin treatments IBA 2000ppm vastly enhances rooting and sprouting behavior followed by IPA, IBA+NAA and NAA of Excoecaria agallocha. The isoenzyme analysis for peroxidase were clearly shown that peroxidase (POX) were highly supports both root initiation and elongation processes in Excoecaria agallocha L.

کلیدواژه‌ها

Excoecaria agallocha؛ Mangrove؛ Stem cutting؛ Auxins؛ Peroxidase isoenzymes

مراجع

Arnon, D.I. 1949. ̍Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vularis L.̍ Plant Physiol. 24: 1-15.

Basak, U.C., A.B. Das, and P. Das. 1995. ̍Metabolic changes during rooting in stem cutting of five mangrove species̍. Plant growth regul.17: 141-148.

Blakely L.M., R.M. Blakeley, P.M. Colowit, and D.S. Elliott. 1988. ̍Experimental studies on lateral root formation in radish seedling roots. II. Analysis of dose response to exogenous auxin. ̍ Plant Physiology 87:414-9.

Chen LM, J.T. Cheng, E.L. Chen, T.J. Yiu, and Z.H. Liu. 2002a. ̍Naphthaleneacetic acid suppresses peroxidase activity during the induction of adventitious roots in soybean hypocotyls. ̍ J. Plant Physiol Biochem 40: 33-39.

Chien-Hung chou, Yi-Chi Huang, and Liu. Zin-Huang. 2010. ̍Peroxidase genes differentially respond to Auxin during the formation of adventitious roots in soybean hypocotyls̍. Plant growth regul. 60: 151-161.

 Das, P., U.C. Basak, and A.B. Das. 1997a. ̍Metabolic changes during rooting in pre-girdled stem cuttings   and air layers of Hertiera.̍ Bot. Bull Acad Sin (Taipei) 38: 91-95.

Das, P., U.C. Basak, and A.B. Das. 1997b. ̍Restoration of the mangrove vegetation in the Mahanadi delta, Orissia, India. ̍ Mangroves and salt marshes 1: 155-161.

Davies, P.J. 1996. ̍ Plant hormones and their role in plant growth and development. ̍ Dordrecht, Netherlands: Kluwer Academic Publishers.

Davis, T.D., N. Sankhla, and A. Upadhyaya. 1986. ̍Paclobutrazol: A promising plant growth regulator. In: Purohit, S.S., (ed.). Hormonal regulation of plant growth and development. ̍ Agro Botanical publishers, Bikaner, India. Vol-III: pp. 311-331.

De Klerk, G.J., W. Van der Krieken, and J.C. De Jong. 1999. ̍The formation of adventitious roots: new concepts, new possibilities. ̍ In Vitro cell. Dev. Biol. Plant 35, 189-199.

Faisal. M., and M. Anis. 2003. ̍Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. ̍ Plant cell, Tissue organ cult. 75: 125-129.

FAISAL, M. and M. ANIS. 2006. ̍Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia. ̍ BIOLOGIA PLANTARUM 50 (3): 437-440.

Ghani, A. 2003. ̍Medicinal plants of Bangladesh. ̍The Asiatic Society of Bangladesh. 228-229.

Ghosh, S.K. and R. N. Basu. 1974. ̍Metabolic changes during the regeneration of roots on cuttings. ̍ Indian J. Exp. Biol. 12: 166-168.

Gunes, T. 2000. ̍Peroxidase and IAA oxidase activity during rooting of poplar species. ̍ Turkish Journal of Botany 24: 97-101.

Hogarth, and J. Peter.1999. The biology of mangroves New York: Oxford University Press.

Kathiresan, K., and B.L. Bingham. 2001.̍ Biology of mangrove and mangrove ecosystems. ̍ Adv mar Bio 2001; 40: 81-251.

Kaur, S., S.S. Cheema, B.R. Chhabra, and K.K. Talwar. 2002. ̍Chemical induction of physiological changes during adventitious root formation and bud break in grapevine cuttings. ̍Plant Growth Regulation 37: 63-68.

Kaya, C., A. Levent Tuna, A. Alfredo, and C. Alves. 2006. ̍Gibberellic acid improves water deficit tolerance in maize plants. ̍ Acta Physiol. Plant. 28: 331-337.

Laemmli, U.K. 1970. ̍Cleavage of structural proteins during the assembly of the head of bacteriophage T4. ̍ Nature,227:680-5.

Nag, S., K. Saha, and M. A. Choudhuri. 2001. ̍Role of Auxin and polyamines in adventitious root formation in relation to changes in compounds involved in rooting. ̍ J. Plant growth Regul. 20, 182-194.

Neogy, M., J.K. Datta, S. Mukherji, and A.K. Roy. 2001. ̍Effect of aluminum on pigment content, hill activity and seed yield in mung-bean. ̍ Indian J. Plant Physiol. 6 381–385.

Nordstrom, A.C., F.A. Jacobs, and L. Eliasson. 1991. ̍Effect of exogenous indole-3-acetic acid and indole-3-buturic acid on interval levels of the respective auxins and their conjugation with aspartic acid during adventitious root formation in pea cuttings. ̍ Plant Physiol. 96, 856-861.

Normanly, J. 2010. ̍Approaching cellular and molecular resolution of auxin biosynthesis and metabolism. ̍ Cold Spring Harb. Perspect. Biol. 2, a001594.

Palanisamy, K., S. A. Ansari, Pramod kumar, and B. N. Gupta. 1998. ̍Adventitious rooting in shoot cuttings of Azadirachta indica and Pongamia pinnata. ̍New Forests. 16: 81-88.

Peter K.L.N., and N.A. Sivasothi. 1999. ̍Guide to the Mangroves of Singapore I: The ecosystem and plant Diversity. ̍ Singapore Science centre. 111-112.

Reddy K.K, K. Venkaiah., and B. Bramaramba. 1994. ̍Rooting of stem cuttings of Avicennia officinalis Linn. and Avicennia alba Bl.: A tool for afforestation of blanks in mangrove forests.̍ India Forester,156-161.

Saenger, P. 2002. ̍Mangrove ecology, silvic culture, and conservation. ̍ Dordrecht: Kluwer Academic Publishers.

Sandilyan, S., and K., Kathiresan, 2012. ̍Mangrove conservation: a global perspective. ̍ Biodivers. Conserv. 21 (14), 3523e3542. http://dx.doi.org/10.1007/s10531-012-0388.

Selvam, V., Eganathan, V..M, Karunagaran, T. Ravishankar, and R. Ramasubramanian. 2004. Mangrove plants of Tamil Nadu. M.S. Swaminathan research foundation.

Sunita Kochhar, S.P. Singh, and V.K. Kochhar. 2008. ̍Effect of auxins and associated biochemical changes during clonal propagation of the biofuel plant-Jatropha curcas.̍ Biomass and Bioenergy, 32: 1136-1143.

Tomlinson, P.B. 1986. ̍The Botany of Mangroves. ̍ Cambridge University 419.

Trettin, C.C., C.E. Stringer, and S.J. Zarnoch. 2016. ̍Composition, biomass and structure of mangroves within the Zambezi River Delta. ̍Wetlands Ecology and Management24:173e186.

Woodward, A.W., and B. Bartel. 2005. Auxin: regulation and interaction. ̍ Annals of Botany 95:707-35.

Zhang, Y., W. Wang, Q. Wu, B. Fang, and P. Lin. 2006. ̍The growth of Kandelia candel seedlings in mangrove habitats of the Zhang jiang estuary in Fujian, China. ̍Acta Ecologica Sinica,26:1648e1656.