مطالعه خصوصیات بیولوژیکی، ترکیبی و توپوگرافی سطحی داربست گرانوله هیدروکسی آپاتیت/ پوسته تخم شتر مرغ برای مهندسی بافت استخوان

فصلنامه زیست شناسی جانوری

دوره 13، شماره 3، خرداد 1400، صفحه 27-40   

نوع مقاله: مقالات پژوهشی

نویسندگان

فریده اله گاهی¹؛ اسماعیل فتاحی^{* 1}؛ محسن سعیدی²؛ میر محمود مرتضوی رودمیانه³

¹گروه زیست شناسی، واحد آیت ا... آملی، دانشگاه آزاد اسلامی، آمل، ایران

²مرکز تحقیقات سلول بنیادی ، دانشگاه علوم پزشکی گلستان، گرگان، ایران

³گروه تحقیقاتی بیومدیکال، انیستیتوی پژوهشی چیترا، دانشگاه پزشکی کرالاSCTIMST ، تیرو وانانتاپورام، هندوستان

چکیده

هدف از این مطالعه بررسی شکل هندسی و توانایی این داربست در ترکیب با سلول مزانشیمی و شبه فیبرو بلاستی و سنجش واکنش سمیت آن نسبت به سلول می­باشد. بدین لحاظ چند نسبت متفاوت از داربست هیدروکسی آپاتیت/ پوسته تخم شتر مرغ ایجاد گردید تا بتوان خصوصیات سطحی و پاسخگویی سلول را نسبت به انواع مختلف ترکیبی هیدروکسی آپاتیت/پوسته تخم شتر مرغ (OsE/HA) (نسبت­های 0-1، 1-2، 1-1، 2-، 1-0)  سنجید.  بیومتریال­های طبیعی منجمله پوسته تخم شترمرغ  که دارای مواد معدنی مثل کربنات کلسیم نقش عمده­ای در ساخت داربست مربوط به بافت­های سخت و تحقیقات امروزه را ایفا می­کنند. مشخصه یابی سطحی با استفاده از SEM و خصوصیات ترکیبی داربست بوسیله XRD  و FTIR مورد بررسی قرار گرفت و در نهایت برای کاربرد این ترکیب در بافت­های بیولوژیکی تست تماس مستقیم (سمیت) به­همراه سلول­های شبه فیبروبلاستی (L929) انجام گرفت. بعد از تست سمیت داربست آماده تست­های درون آزمایشگاهی (اینویترو) پذیرش سلول­های بنیادی مزانشیمی جدا شده از بافت چربی خرگوش سفید نیوزیلندی (RADMSCs) می­­گردد. نتایج این پژوهش نشان داد که این داربست دارای بهترین زیست­سازگاری و بیشترین لاکوناهای سطحی برای جذب سلول به خود در گروه هیدروکسی­آپاتیت/ پوسته تخم شتر مرغ با نسبت 2-1 می­باشد و این ترکیب بیومتریال طبیعی با مواد سنتتیک می­تواند در پیشبرد مهندسی  بافت استخوان کاربرد فراوان داشته باشد.

کلیدواژه‌ها

مهندسی بافت؛ داربست هیدروکسی آپاتیت؛ پوسته تخم؛ شترمرغ؛ سلول شبه فیبرو بلاستی

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