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Bozkurt, Yusuf, Yavas, İlker. (1400). Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 13(2), 147-154. doi: 10.22034/iar.2021.1926852.1151
Yusuf Bozkurt; İlker Yavas. "Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation". نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 13, 2, 1400, 147-154. doi: 10.22034/iar.2021.1926852.1151
Bozkurt, Yusuf, Yavas, İlker. (1400). 'Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation', نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 13(2), pp. 147-154. doi: 10.22034/iar.2021.1926852.1151
Bozkurt, Yusuf, Yavas, İlker. Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation. نشریه علمی پژوهشی دانشگاه آزاد اسلامی, 1400; 13(2): 147-154. doi: 10.22034/iar.2021.1926852.1151

Effect of supplementations of docosahexaenoic acid (DHA) into a Tris-glucose based extender on the post-thaw sperm quality, fertility and hatching rates in brown trout (Salmo trutta macrostigma) following cryopreservation

International Aquatic Research
دوره 13، شماره 2، شهریور 2021، صفحه 147-154    اصل مقاله (537.83 K)
نوع مقاله: Short communication
شناسه دیجیتال (DOI): 10.22034/iar.2021.1926852.1151
نویسندگان
Yusuf Bozkurt* 1؛ İlker Yavas2
1İskenderun Technical University, Faculty of Marine Sciences and Technology, Department of Aquaculture, Turkey
2Hatay Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Turkey
چکیده
The supplementation of extenders with PUFAs has not been investigated in fish by now. Thus, the present study examined whether different levels of docosahexaenoic (DHA), which is the predominat PUFAs in sperm plasma membrane, in Tris-glucose based extender could increase the quality and fertilizing ability of brown trout (Salmo trutta macrostigma) sperm following cryopreservation were evaluated. Sperm samples diluted (1:10) with the base extender were supplemented with 1, 2.5, 5, 7.5 and 10 ng mL-1 concentrations of DHA. In the control group, sperm was diluted in the base extender, without DHA. Following dilution, the sperm was aspirated into 0.25 mL straws, frozen 3 cm above of the liquid nitrogen (LN2) surface, and plunged directly into the LN2. Based on the results, increasing of DHA supplementations in the Tris-glucose based extender improved post-thaw progressive motility, progressive duration of motility, viability, and fertility compared to the control group (p <0.05). Additionally, the results indicated that supplementation of 7.5 ng mL-1 of DHA significantly exhibited the best cryoprotective effect in terms of progressive motility (62.4±1.73%), progressive duration of motility (46.8±1.32s), viability (56.2±1.20%), fertility (52.6±1.48%), and hatching (28.6±1.45%). The results showed that supplementation of DHA to the Tris-glucose based extender can be beneficial for the cryopreservation of brown trout sperm.
کلیدواژه‌ها
Cryopreservation؛ Sperm؛ Extender؛ DHA؛ Brown trout
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