Intra- and inter-specific variability of IGF-I expression in farmed cold and warm-water species in Iran

International Aquatic Research

دوره 13، شماره 2، شهریور 2021، صفحه 155-162   

نوع مقاله: Short communication

شناسه دیجیتال (DOI): 10.22034/iar.2021.682480

نویسندگان

Maryam Haghi¹؛ Mahmoud Mohseni^{* 2}؛ Masoud Ghane³

¹Department of Fisheries, Tonekabon branch, Islamic Azad University, Tonekabon, Iran

²International Sturgeon Institute, Rasht, Iran

³Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran

چکیده

Changes in the expression of liver insulin-like growth factor IGF-I mRNA in two growing cold-water fish species Oncorhynchusmykiss(one from locally produced and one from imported broodstock) and in two warm-water species, Hypophthalmichthysnobilis and Hypophthalmichthysmolitrix were analyzed. Gene expression analysis in liver tissues by using real-time PCR revealed a significant difference in expression level of IGF-I between native and non-native rainbow trout (P < 0.05).  IGF-I levels were found to have the following order: non-native rainbow trout> bighead carp> silver carp> native rainbow trout. Local strains of rainbow trout, bighead carp and silver carp exhibited stable IGF-I mRNA levels, at different stages of growth. The imported broodstock of rainbow trout exhibited increased IGF-I levels during the entire period studied, with a peak in the IGF-I mRNA levels of fingerlings. The results indicate intra- and inter-specific variability of IGF-I expression in farmed fish.

کلیدواژه‌ها

Fish growth؛ Oncorhynchus mykiss؛ Hypophthalmichthys nobilis؛ Hypophthalmichthys molitrix؛ IGF-I mRNA gene expression